Impact of combined long-term fructose and prednisolone intake on glucose and lipid homeostasis in rats: benefits of intake interruption or fish oil administration

Santos, Cristiane dos; Silva, Julia Spanhol da; Brunetta, Henver Simionato; Chagas, Thays Rodrigues; Zoccal, Daniel B.; Nunes, Everson Araújo; Rafacho, Alex

doi:10.1016/j.jnutbio.2020.108572

Cited by 4 publications

(4 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Liver protein extracts were prepared using RIPA lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, 1% NP‐40) containing a cocktail of protease inhibitors (Sigma Aldrich, cat. code #P8340), adapted from 29 . After homogenization and centrifugation at 14 000× for 30 minutes at 4℃, the supernatant was used for the determination of protein concentration with Bradford reagent (Bio‐Rad, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…All pancreas tissue from two interspaced sections, as described before, were selected for endocrine pancreas morphology analysis. The entire sections were scanned using the Axio Scan slide scanner (Zeiss, Germany), and ZenLite software‐Blue edition (Zeiss) was used for image analysis as described before 29 . The relative mass of islets (%), β‐cells (insulin immunostained), α‐cells (glucagon immunostained), and δ‐cells (somatostatin immunostained) was calculated by dividing their respective total immunostained areas (μm 2 ) by the total pancreatic section area (μm 2 ) and then multiplying by the total pancreas mass (mg).…”

Section: Methodsmentioning

confidence: 99%

“…The entire sections were scanned using the Axio Scan slide scanner (Zeiss, Germany), and ZenLite software-Blue edition (Zeiss) was used for image analysis as described before. 29 The relative mass of islets (%), β-cells (insulin immunostained), αcells (glucagon immunostained), and δ-cells (somatostatin immunostained) was calculated by dividing their respective total immunostained areas (μm 2 ) by the total pancreatic section area (μm 2 ) and then multiplying by the total pancreas mass (mg). The proportion of β-cells, α-cells, and δ-cells per islets was calculated by dividing the total area of the cells immunostained for insulin, glucagon or somatostatin, respectively, by the total area of the islet, and expressed as %.…”

Section: Pancreas Morphologymentioning

confidence: 99%

“…code #P8340), adapted from. 29 After homogenization and centrifugation at 14 000× for 30 minutes at 4℃, the supernatant was used for the determination of protein concentration with Bradford reagent (Bio-Rad, CA, USA). About 30 μg of protein extracts were separated on 10% polyacrylamide gels and transferred to nitrocellulose membranes.…”

Section: Protein Quantification and Immunoblotmentioning

confidence: 99%

See 3 more Smart Citations

Excess of glucocorticoids during late gestation impairs the recovery of offspring’s β‐cell function after a postnatal injury

et al. 2021

Self Cite

View full text Add to dashboard Cite

Since prenatal glucocorticoids (GC) excess increases the risk of metabolic dysfunctions in the offspring and its effect on β-cell recovery capacity remains unknown we investigated these aspects in offspring from mice treated with dexamethasone (DEX) in the late pregnancy. Half of the pups were treated with streptozotocin (STZ) on the sixth postnatal day (PN). Functional and molecular analyses were performed in male offspring on PN25 and PN225. Prenatal DEX treatment resulted in low birth weight. At PN25, both the STZ-treated offspring developed hyperglycemia and had lower β-cell mass, in parallel with higher α-cell mass and glucose intolerance, with no impact of prenatal DEX on such parameters. At PN225, the β-cell mass was partially recovered in the STZ-treated mice, but they remained glucose-intolerant, irrespective of being insulin sensitive. Prenatal exposition to DEX predisposed adult offspring to sustained hyperglycemia and perturbed islet function (lower insulin and higher glucagon response to glucose) in parallel with exacerbated glucose intolerance. β-cell-specific knockdown of the Hnf4α in mice from the DS group resulted in exacerbated glucose intolerance. We conclude that high GC exposure during the prenatal period exacerbates the metabolic dysfunctions in adult life of mice exposed to STZ early in life, resulting in a lesser ability to recover the islets' function over time.This study alerts to the importance of proper management of exogenous GCs during 2 of 17 | DOS SANTOS eT Al.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Pancreas Morphologymentioning

confidence: 99%

Section: Protein Quantification and Immunoblotmentioning

confidence: 99%

See 2 more Smart Citations