Comparing the osteogenic potential of schneiderian membrane and dental pulp mesenchymal stem cells: an in vitro study

Berbéri, Antoine; Sabbagh, Joseph; Assaf, Rita Bou; Ghassibe-Sabbagh, Michella; Al-Nemer, Fatima; Majzoub, Rania El; Fayyad‐Kazan, Mohammad; Badran, Bassam

doi:10.1007/s10561-020-09887-4

Cited by 5 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Anatomically, the maxillary sinus floor area is a complex structure, and the formation of new bone can be induced by mesenchymal stem cells derived from the maxillary sinus mucosa and osteogenic differentiation with mineralization [ 14 ]. Emerging evidence indicates that human maxillary sinus stem cells may have multipotency differentiation features, which may contribute to osteogenic differentiation [ 7 , 15 ]. Based on cell culture and differentiation model in vitro , we found that human maxillary sinus mucosal-derived cells appeared fibroblastic, and the cells showed an osteoblastic or osteoprogenitor appearance after osteogenic induction.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of osteogenic differentiation of human maxillary sinus mesenchymal stem cells using RNA-Seq

Zhou,

Jiang,

Zeng

et al. 2023

Heliyon

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of osteogenic differentiation of human maxillary sinus mesenchymal stem cells using RNA-Seq

Zhou,

Jiang,

Zeng

et al. 2023

Heliyon

View full text Add to dashboard Cite

“…Subsequently, MSCs located in the human maxillary sinus membrane (hMSM) were discovered (Srouji et al, 2009). Further studies confirmed that MSCs derived from hMSM could be cultured in vitro and induced to differentiate towards osteogenesis (Berbéri et al, 2017(Berbéri et al, , 2021Chen et al, 2022;Cho et al, 2014;Graziano et al, 2012;Guo et al, 2015;Kim et al, 2009;Ren et al, 2022;Srouji et al, 2009Srouji et al, , 2010. The isolation and analyses of MSCs from hMSM could be helpful in clarifying the osteogenic mechanism of hMSM and designing targeted therapeutic strategies.…”

Section: Introductionmentioning

confidence: 99%

Isolation, identification and osteogenic capability analysis of mesenchymal stem cells derived from different layers of human maxillary sinus membrane

Lv,

Xu,

Wang

et al. 2024

J Clinic Periodontology

View full text Add to dashboard Cite

AimTo discover the populations of mesenchymal stem cells (MSCs) derived from different layers of human maxillary sinus membrane (hMSM) and evaluate their osteogenic capability.Materials and MethodshMSM was isolated into a monolayer using the combined method of physical separation and enzymatic digestion. The localization of MSCs in hMSM was performed by immunohistological staining and other techniques. Lamina propria layer‐derived MSCs (LMSCs) and periosteum layer‐derived MSCs (PMSCs) from hMSM were expanded using the explant cell culture method and identified by multilineage differentiation assays, colony formation assay, flow cytometry and so on. The biological characteristics of LMSCs and PMSCs were compared using RNA sequencing, reverse transcription and quantitative polymerase chain reaction, immunofluorescence staining, transwell assay, western blotting and so forth.ResultsLMSCs and PMSCs from hMSMs were both CD73‐, CD90‐ and CD105‐positive, and CD34‐, CD45‐ and HLA‐DR‐negative. LMSCs and PMSCs were identified as CD171+/CD90+ and CD171−/CD90+, respectively. LMSCs displayed stronger proliferation capability than PMSCs, and PMSCs presented stronger osteogenic differentiation capability than LMSCs. Moreover, PMSCs could recruit and promote osteogenic differentiation of LMSCs.ConclusionsThis study identified and isolated two different types of MSCs from hMSMs. Both MSCs served as good potential candidates for bone regeneration.

show abstract

“…However, the maxillary sinus floor mucosa contains mesenchymal stem cells and has osteogenic differentiation potential. In this regard, the destruction of its integrity will affect osteogenic effects around the implant to a certain degree 20–23 . Accordingly, it is necessary to maintain the integrity of the maxillary sinus membrane and prevent perforation of the maxillary sinus mucoperiosteum during surgery.…”

Section: Introductionmentioning

confidence: 99%

Modified internal sinus elevation for patients with low residual bone height: A retrospective clinical study

Deng

Tong

Gao

et al. 2023

Clin Implant Dent Rel Res

View full text Add to dashboard Cite

Background: We have modified the internal sinus elevation by combining it with the sinus mucoperiosteum stripping procedure, which further increases the indications for the internal lift. Similar long-term clinical follow-up studies and three-dimensional finite element analyses are rare.Objective: This study aimed to investigate the feasibility of the modified internal sinus floor elevation method in patients with low residual bone height using a three-dimensional (3D) finite element model and report on the long-term outcomes.Materials and Methods: Overall, 99 implants were placed in 86 patients. All patients were followed-up for 3-24 months. The modified internal sinus floor elevation was dynamically simulated using a 3D finite element model, and the stress of the sinus membrane was measured.Results: In trial group A (modified internal sinus floor elevation group), 57 implants were placed in 52 patients. The sinus floor height was lifted by 6.5 mm (95%confidence interval (CI): 6.2-6.8). The perforation rate was 8.8%, and the implant survival rate was 96.5%. In control group B (external sinus floor elevation group), 42 implants were placed in 34 patients. The sinus floor height was lifted by 8.8 mm (95%CI: 8.4-9.3). The perforation rate was 14.3%, and the implant survival rate was 100%. In trial group A, compared with the control group B, perforation decreased by 5.5% (odds ratio = 0.50 and 95%CI: 0.14-1.78; p = 0.282), and the sinus floor lift height was 2.3 mm lower (95%CI, 1.8-2.9; p < 0.001). The finite element analysis showed that the peak stress of the sinus membrane increased with an increase in height elevation and degree of membrane separation. Conclusion:Our findings indicate the positive clinical outcomes in patients with low RBH associated with the modified internal sinus elevation procedure.

show abstract

Comparing the osteogenic potential of schneiderian membrane and dental pulp mesenchymal stem cells: an in vitro study

Cited by 5 publications

References 31 publications

Transcriptome analysis of osteogenic differentiation of human maxillary sinus mesenchymal stem cells using RNA-Seq

Transcriptome analysis of osteogenic differentiation of human maxillary sinus mesenchymal stem cells using RNA-Seq

Isolation, identification and osteogenic capability analysis of mesenchymal stem cells derived from different layers of human maxillary sinus membrane

Modified internal sinus elevation for patients with low residual bone height: A retrospective clinical study

Contact Info

Product

Resources

About