Cell-based 3D bionic screening by mimicking the drug–receptor interaction environment in vivo

Abstract

A cell-based screening method for drug lead target capture using a microcarrier covered with highly expressed receptor cells as a 3D biomimetic framework.

Unraveling the mystery of efficacy in Chinese medicine formula: New approaches and technologies for research on pharmacodynamic substances

“…Compared to traditional 2D cells, 3D cells have a higher degree of cell-to-cell interaction and maintains a more ordered tissue processing of the tiny structures of organs in vivo ( Barbosa et al, 2021 ). 3D cell culture has made many promising achievements since its introduction ( Booij et al, 2019 , Froehlich et al, 2016 , Wei et al, 2021a ). Current research achieved an effective anti-asthma herbal drug evaluation depending on 3D airway smooth muscle cell arrays and gel imaging system.…”

A review for cell-based screening methods in drug discovery

“…Due to the use of microcarriers for 3D cell culture, the stationary phase loaded into the column also presents 3D characteristics. Combing with HPLC/MS, active compounds can be bionically screened and identified successfully (Wei et al 2021 ). Although cell-based chromatography can more likely screen active lead drugs, it lacks the function of predicting cellular effects after screening and identification, and needs to combine with the cell-based assay for further activity verification.…”

Supramolecular Responsive Chitosan Microcarriers for Cell Detachment Triggered by Adamantane

“…Microcarriers are 100 to 300 micron supporting matrices that permit the growth of adherent cells in bioreactor systems. They have a larger surface area to volume ratio in comparison to single cell monolayers, enabling cost-effective cell production and expansion [1][2][3][4]. Microcarriers are composed of a solid matrix that must be separated from expanded cells during downstream processing stages.…”