Transcriptome-wide changes associated with the reproductive behaviour of male guppies exposed to 17α-ethinyl estradiol

Saaristo, Minna; Craft, John A.; Tyagi, Sonika; Johnstone, Christopher P.; Allinson, Mayumi; Ibrahim, Khalid S.; Wong, Bob B. M.

doi:10.1016/j.envpol.2020.116286

Cited by 6 publications

(1 citation statement)

References 102 publications

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“…In males, the E 2 level was significantly increased. On the one hand, E 2 may act on the brain and affect mating behavior, which is consistent with the results from brain transcriptomic data after EE2 exposure in guppies (Saaristo et al 2021 ). On the other hand, E 2 can regulate ptger expression levels (Blesson et al 2012 ), and PGE 2 may have an influence on sperm mobility (Carlson et al 2022 ; Kennedy et al 2003 ).…”

Section: Discussionsupporting

confidence: 90%

Mating behaviors in ovoviviparous black rockfish (Sebastes schlegelii): molecular function of prostaglandin E2 as both a hormone and pheromone

Lyu,

Yao,

Xie

et al. 2024

Mar Life Sci Technol

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Prostaglandins (PGs) are profound hormones in teleost sexual behavior, especially in mating. PGs act as pheromones that affect the olfactory sensory neurons of males, inducing the initiation of a series of mating behaviors. However, the molecular mechanism by which PGs trigger mating behavior in ovoviviparous teleosts is still unclear. In the present study, we employed the ovoviviparous black rockfish (Sebastes schlegelii), an economically important marine species whose reproductive production is limited by incomplete fertilization, as a model species. The results showed that when the dose of PGE2 was higher than 10 nmol/L, a significant (P < 0.05) increase in mating behaviors was observed. Dual-fluorescence in situ hybridization indicated that PGE2 could fire specific neurons in different brain regions and receptor cells in the olfactory sac. After combining with specific neurons in the central nervous system (CNS), a series of genes related to reproduction are activated. The intracerebroventricular administration of PGE2 significantly increased lhb levels (P < 0.05) in both sexes. Moreover, steroidogenesis in gonads was also affected, inducing an increase (P < 0.05) in E2 levels in males and T levels in females. PGE2 levels were also increased significantly (P < 0.05) in both sexes. The present study revealed that PGE2 can activate mating behavior in black rockfish in both hormone and pheromone pathways, leading to variations in sex steroid levels and activation of reproductive behaviors. Our results provide not only novel insight into the onset of mating behaviors in ovoviviparous teleosts but also solutions for the incomplete fertilization caused by natural mating in cage aquaculture.

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