Prevalence and antimicrobial susceptibility of Campylobacter isolated from retail chickens in Thailand

Wangroongsarb, Piyada; Cheunban, Nattapong; Jittaprasatsin, Chutima; Kamthalang, Thanitchai; Saipradit, Nonglak; Chaichana, Pattharaporn; Pulsrikarn, Chaiwat; Parnmen, Sittiporn; Sripichai, Orapan

doi:10.1016/j.ijfoodmicro.2020.109017

Cited by 18 publications

(11 citation statements)

References 48 publications

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“…In the present study, the prevalence of SUL- and TMP-resistance was low in E. coli isolates from farm B, although qnrS -positive isolates were obtained from this farm at a rate comparable to that in farm A. Thus, the prevalence of E. coli with PMQR may be caused by the use of quinolones in poultry in Thailand ( Nhung et al, 2016 ; Wangroongsarb et al, 2021 ) and the association of the combined use of sulfadiazine and TMP at farm A with the selection of E. coli with TMP resistance and PMQR is likely to be limited. Because only 2 farms participated in the present study, it is important to conduct large-scale studies to evaluate the prevalence of PMQR in E. coli originating from chickens in Thailand.…”

Section: Discussionmentioning

confidence: 57%

“…The inappropriate use of antimicrobial medications important to humans in animals and the in-feed use of these drugs are major concerns. Quinolones, including fluoroquinolones are categorized as “highest priority critically important” antimicrobials for human medicine ( WHO, 2019 ) and have been used in food animals in many countries ( Collignon et al, 2016 ; Roth et al, 2019 ), including poultry in Thailand ( Nhung et al, 2016 ; Wangroongsarb et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim

2022

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One hundred and twenty chicken samples from feces (n = 80), the carcass surface at slaughter at 2 meat chicken farms (n = 20), and retail chicken meat from 5 markets (n = 20) collected during 2018 and 2019 were examined for the prevalence of plasmid-mediated quinolone resistance ( PMQR ) in Escherichia coli . We detected qnrS -positive E. coli in a total of 74 samples from feces (n = 59), the carcass surface (n = 7), and retail meat (n = 8). These 74 qnrS -positive isolates were tested for antimicrobial susceptibility to determine the minimum inhibitory concentrations ( MICs ) of certain antimicrobials and genetically characterized. Ampicillin-resistance accounted for 71 of the 74 isolates (96%), followed by resistance to oxytetracycline (57/74; 77%), enrofloxacin ( ERFX ) (56/74; 76%), sulfisoxazole ( SUL ) (56/74; 76%), trimethoprim ( TMP ) (49/74; 66%), and dihydrostreptomycin (48/74; 65%). All farm-borne SUL- and TMP-resistant isolates except one were obtained from samples from farm A where a combination of sulfadiazine and TMP was administered to the chickens. Concentrations of ERFX at which 50 and 90% of isolates were inhibited were 2 μg/mL and 32 μg/mL, respectively. Diverse pulsed-field gel electrophoresis ( PFGE ) patterns of Xba I-digested genomic DNA were observed in the qnrS -positive isolates from fecal samples. Several isolates from feces and the carcass surface had identical Xba I-digested PFGE patterns. S1-nuclease PFGE and Southern blot analysis demonstrated that 7 of 11 dfrA13 -positive fecal isolates carried both the qnrS and dfrA13 genes on the same plasmid, and 2 of 3 dfrA1 -positive isolates similarly carried both qnrS and dfrA1 on the same plasmid, although the PFGE patterns of Xba I-digested genomic DNA of the isolates were different. These results suggest that the qnrS gene is prevalent in chicken farms via horizontal transfer of plasmids and may partly be co-selected under the use of TMP.

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Section: Discussionmentioning

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim

2022

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“…Campylobacter contamination in the chicken production chain and retail meat varies greatly between nations, necessitating the development of country-specific intervention strategies ( Wangroongsarb et al, 2021 ). According to a collection of prevalence statistics from several Asian research, over 60% of Asian countries ( Suzuki and Yamamoto, 2009 ) are affected by Campylobacter contamination.…”

Section: Discussionmentioning

confidence: 99%

“…C. jejuni is becoming increasingly resistant to clinically important antibiotics, and drug-resistant strains can be transmitted to humans through the food chain; this situation constitutes a major public health problem ( Wangroongsarb et al, 2021 ). In this study, chicken C. jejuni isolates had high resistance to quinolones and β-lactams with rates similar to those reported in previous studies (approximately 80%) ( Luangtongkum et al, 2009 ).…”

Section: Discussionmentioning

confidence: 99%

Prevalence, drug resistance spectrum and virulence gene analysis of Campylobacter jejuni in broiler farms in central Shanxi, China

Yang¹,

Li²,

Dong³

et al. 2023

Poultry Science

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“…Campylobacters are the most frequently isolated enteric bacterial pathogens in both developed countries and low- and middle-income countries (LMICs) [ 9 ]. The genus of Campylobacter includes 17 species and 6 subspecies; the thermotolerant species C. jejuni and C. coli are the best known human pathogens causing human gastroenteritis [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Campylobacter spp. in Eggs and Laying Hens in the North-East of Tunisia: High Prevalence and Multidrug-Resistance Phenotypes

Gharbi

Béjaoui

Hamda

et al. 2022

Veterinary Sciences

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Despite the importance of eggs in the human diet, and unlike other products, for which food safety risks are widely investigated, information on the occurrence of Campylobacter and antimicrobial resistance in eggs and layer hen flocks is lacking in Tunisia. This study was conducted to determine the occurrence of Campylobacter and the antimicrobial resistance in layer hens and on eggshells. Thus, 366 cloacal swabs and 86 eggshell smear samples were collected from five layer hen farms in the North-East of Tunisia. The occurrence of Campylobacter infection, and the antimicrobial resistance rates and patterns, were analyzed. The occurrence rates of Campylobacter infection in laying hens and eggshells were 42.3% and 25.6%, respectively, with a predominance of C. jejuni (68.4%, 81.9%), followed by C. coli (31.6%, 18.2%). The antimicrobial susceptibility testing revealed high resistance rates against macrolides, tetracycline, quinolones, β-lactams, and chloramphenicol, with percentages ranging from 35.5% to 100%. All isolates were multidrug resistant (MDR) and five resistance patterns were observed. These results emphasized the risk to consumer health and the need to establish a surveillance strategy to control and prevent the emergence and the spread of resistant strains of Campylobacter in poultry and humans.

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Prevalence and antimicrobial susceptibility of Campylobacter isolated from retail chickens in Thailand

Cited by 18 publications

References 48 publications

Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim

Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim

Prevalence, drug resistance spectrum and virulence gene analysis of Campylobacter jejuni in broiler farms in central Shanxi, China

Campylobacter spp. in Eggs and Laying Hens in the North-East of Tunisia: High Prevalence and Multidrug-Resistance Phenotypes

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