Validation of an
            <i>in vitro</i>
            system to trigger changes in the gene expression of effectors of
            <i>Sclerotinia sclerotiorum</i>

Maximiano, Mariana Rocha; Miranda, Vívian de Jesus; Barros, Everaldo Gonçalves de; Dias, Simoni Campos

doi:10.1111/jam.14973

Cited by 5 publications

(1 citation statement)

References 70 publications

(142 reference statements)

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“…qRT-PCR was performed according to the manufacturer's protocol in a 7300 Real-Time PCR System (Applied Biosystems). Reactions were made in 12 μL containing 5 μL of Fast SYBR® Green Master Mix (Applied Biosystems), 2 μL cDNA (diluted 20 ×) probably corresponding to approximately 10 ng, and 0.2 μM of each primer (forward and reverse) [15][16][17][18]. Reactions were performed with three biological replicates and run in experimental triplicates using the manufacturer's recommended cycling parameters (holding stage: 95 °C for 10 min, cycling stage: 40 cycles of 95 °C for 15 s (denaturation), and 60 °C for 1 min (annealing and extension)).…”

Section: Qrt-pcr Experiments and Data Analysismentioning

confidence: 99%

Priming of defense-related genes in Brassica oleracea var. capitata using concentrated metabolites produced by Rhizobium tropici CIAT 899

et al. 2022

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To verify the potential of metabolites extracted from Rhizobium tropici to trigger the priming of defense responses in cruciferous plants, we analyzed the expression of defense-related genes by qRT-PCR. Brassica oleracea var. capitata, susceptible to Xanthomonas campestris pv. campestris, were grown in greenhouse conditions. At 18 days after sowing, plants were inoculated with 1 mL of 1% concentrated metabolites produced by R. tropici (CM-RT) in the root. In a second experiment, leaves were sprayed with 1 mL of a solution containing 1% CM-RT. Aerial and root tissue were collected separately at 0 (non-treated control condition), 24, and 48 h after application, submitted to RNA extraction and gene expression analysis by qRT-PCR. The results showed that, after root treatment with CM-RT, most evaluated genes were upregulated at 24 h after application and downregulated at 48 h after application in roots, while in leaves, genes were downregulated both at 24 and 48 h after application. On the other hand, leaf treatment with CM-RT showed that most evaluated genes in leaves and roots were upregulated at 24 and 48 h after application. These results indicate that the effect of CM-RT applied in roots seems restricted to the applied region and is not sustained, while the application in leaves results in a more systemic response and maintenance of the effect of CM-RT for a longer period. The results obtained in this study emphasize the biotechnological potential of using metabolites of R. tropici as an elicitor of active defense responses in plants.

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Section: Qrt-pcr Experiments and Data Analysismentioning

confidence: 99%

Priming of defense-related genes in Brassica oleracea var. capitata using concentrated metabolites produced by Rhizobium tropici CIAT 899

et al. 2022

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MicroRNA cross-talk between Monilinia fungal pathogens and peach host

Arslan,

Ozkilinc

2024

Phytoparasitica

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The reciprocal targeting of microRNAs (miRNA) and micro-like-RNAs (milRNA) between hosts and pathogens is critical for understanding their interactions. In this study, reciprocal miRNA targets were explored in two Monilinia fungal pathogens, M. fructicola & M. laxa, and their peach host (Prunus presica). Using in silico analysis, 355 and 266 putative miRNAs were predicted for M. fructicola and M. laxa, respectively. Number of miRNAs and their targets differed based on host and pathogen species as 209 M. fructicola miRNAs target 98 peach genes and 128 M. laxa miRNAs target 338 peach genes. On the other hand, peach miRNAs showed the species-specific responses targeting fungal pathways to struggle with its pathogens. These findings indicate distinct strategies and species-specific interactions in this pathosystem. Besides, through the in vitro experimental designs, 166 and 124 expressed miRNAs by M. fructicola were detected in the host-mimicked and control environments, respectively. Additionally, novel miRNAs were discovered, six of which were in the mimicked environment and the seven in the controlled environment as highlighting dynamic and specialized miRNA expression in M. fructicola depending on the environmental conditions. In conclusion, this study provides the first insights into miRNA-mediated interactions between M. fructicola, M. laxa, and peach hosts. Unrevealing the cross talk through the miRNAs in host–pathogen interactions enhances the understanding of pathogenesis and host defense mechanisms. These findings have implications for disease management strategies and contribute to the fields of basic science and evolutionary biology.

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Host induced gene silencing of Sclerotinia sclerotiorum effector genes for the control of white mold

Maximiano

Souza

Santos

et al. 2022

Biocatalysis and Agricultural Biotechnology

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Validation of an in vitro system to trigger changes in the gene expression of effectors of Sclerotinia sclerotiorum

Cited by 5 publications

References 70 publications

Priming of defense-related genes in Brassica oleracea var. capitata using concentrated metabolites produced by Rhizobium tropici CIAT 899

Priming of defense-related genes in Brassica oleracea var. capitata using concentrated metabolites produced by Rhizobium tropici CIAT 899

MicroRNA cross-talk between Monilinia fungal pathogens and peach host

Host induced gene silencing of Sclerotinia sclerotiorum effector genes for the control of white mold

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