2021
DOI: 10.1111/evj.13400
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Successful vitrification of manually punctured equine embryos

Abstract: Background: Successful vitrification of equine expanded blastocysts requires collapse of the blastocoele cavity using a micromanipulator-mounted biopsy pipette on an inverted microscope. Such equipment is expensive and requires user skill.Objectives: To develop a manual method of blastocoele collapse prior to vitrification using commercial products. Study design: In vivo experiment.Methods: Seventy-nine Day 7 or 8 embryos were measured and graded. Twenty were vitrified following micromanipulator-assisted punct… Show more

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Cited by 11 publications
(26 citation statements)
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“…However, this method requires a micromanipulator, which is too expensive and too complicated to become widespread in the equine embryo transfer industry. Very recently good pregnancy rates were obtained with >300 µm embryos that were vitrified after manual blastocoel puncture [ 187 ]. If this is confirmed, this technique can be used on a larger scale and, unlike humans and other ruminants, the freezing of equine embryos may develop rapidly.…”
Section: Comparison Of Assisted Reproduction Techniques In Horses and Humansmentioning
confidence: 99%
“…However, this method requires a micromanipulator, which is too expensive and too complicated to become widespread in the equine embryo transfer industry. Very recently good pregnancy rates were obtained with >300 µm embryos that were vitrified after manual blastocoel puncture [ 187 ]. If this is confirmed, this technique can be used on a larger scale and, unlike humans and other ruminants, the freezing of equine embryos may develop rapidly.…”
Section: Comparison Of Assisted Reproduction Techniques In Horses and Humansmentioning
confidence: 99%
“…14 Subsequently, the conundrum of how to reliably cryopreserve the larger horse blastocysts recovered by flushing on day 7 or 8 was solved by the discovery that the key was reducing the blastocoele fluid volume by puncture and aspiration; indeed, recent studies by Twink and Sandra Wilsher have helped better define the relationship between the diameter of expanded horse blastocysts and the manipulations required to enable successful vitrification. 32,33 Cryopreservation was not the only reason Twink and colleagues wanted to work with very early embryos. Obtaining early embryos, when all or most of the cells were still pluripotent, was essential for the production of genetically identical monozygotic twins.…”
Section: A Ss Is Ted Reproduc Tive Technolog Ie Smentioning
confidence: 99%
“…If small, they do not require capsule perforation (Panzani et al, 2012a;Fanelli et al, 2020). However, if large they might preferentially be punctured before the vitrification step (Hochi et al, 1995;Eldridge-Panuska et al, 2005;Scherzer et al, 2011;Wilsher et al, 2019;Fanelli et al, 2020;Wilsher et al, 2021). In general, the post-ovulation day selected to attempt an embryo recovery influences the percent of embryos collected.…”
Section: Introductionmentioning
confidence: 99%
“…30 uL (Eldridge-Panuska et al, 2005;Fanelli et al, 2020). On the other hand, if the equine embryos are large, besides the capsule perforation (Wilsher et al, 2021) and blastocoelic fluid extraction (Sanchez et al, 2017;Herrera, 2021) they will preferentially require direct contact with liquid nitrogen (Díaz et al, 2016;Sanchez et al, 2017;Herrera, 2021). It seems that with large equine embryos (besides capsule perforation and blastocoelic fluid extraction), when comparing between open and closed vitrification embryo carriers which allow direct and indirect contact with liquid nitrogen during the vitrification step; that the open systems promotes a higher TTI and hence augments the PVSPR (Sanchez et al, 2017).…”
Section: Introductionmentioning
confidence: 99%
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