Porphyra yezoensis polysaccharide and potassium citrate synergistically inhibit calcium oxalate crystallization induced by renal epithelial cells and cytotoxicity of the formed crystals

Sun, Xin-Yuan; Zhang, Hui; Chen, Jia-Yun; Zeng, Guohua; Ouyang, Jian‐Ming

doi:10.1016/j.msec.2020.111448

Cited by 16 publications

(13 citation statements)

References 54 publications

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“…Exposure of renal tubular epithelial cells to COM crystals triggers cell damage, encompassing membrane breakage, cytoskeleton impairment, oxidative stress, and cell apoptosis [13,50,51]. Oxidative stress assumes a pivotal role in kidney disease pathology, with numerous complications attributed to this phenomenon [52]. Enhanced oxidative stress stems from elevated oxidants and decreased antioxidants, contributing to the exacerbation of this imbalance [40,52].…”

Section: Raps Reduced the Cytotoxicity Of Com Crystals And Alleviated...mentioning

confidence: 99%

“…Oxidative stress assumes a pivotal role in kidney disease pathology, with numerous complications attributed to this phenomenon [52]. Enhanced oxidative stress stems from elevated oxidants and decreased antioxidants, contributing to the exacerbation of this imbalance [40,52]. SOD and catalase (CAT), as endogenous antioxidant enzymes, pro ciently scavenge free radicals, upholding cellular oxidative stress equilibrium.…”

Section: Raps Reduced the Cytotoxicity Of Com Crystals And Alleviated...mentioning

confidence: 99%

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Carboxymethylated Rhizoma alismatis polysaccharides reduces the risk of calcium oxalate stone formation by reducing cellular inflammation and oxidative stress

Wang,

Liu,

et al. 2024

Urolithiasis

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This study aims to elucidate the mechanism and potential of Rhizoma alismatis polysaccharides (RAPs) in preventing oxidative damage to human renal proximal tubule epithelial cells. MethodsThe experimental approach involved incubating HK-2 cells with 100 nm calcium oxalate monohydrate for 24 h to establish a cellular injury model. Protection was provided by RAPs with varying carboxyl group contents: 3.57%, 7.79%, 10.84%, and 15.33%. The safeguarding effect of RAPs was evaluated by analyzing relevant cellular biochemical indicators. ResultsFindings demonstrate that RAPs exhibit notable antioxidative properties. They effectively diminish the release of reactive oxygen species, lactate dehydrogenase, and malondialdehyde, a lipid oxidation byproduct. Moreover, RAPs enhance superoxide dismutase activity and mitochondrial membrane potential while attenuating the permeability of the mitochondrial permeability transition pore. Additionally, RAPs signi cantly reduce levels of in ammatory factors, including NLRP3, TNF-α, IL-6, and NO. This reduction corresponds to the inhibition of overproduced pro-in ammatory mediator nitric oxide and the caspase 3 enzyme, leading to a reduction in cellular apoptosis. RAPs also display the ability to suppress the expression of the HK-2 cell surface adhesion molecule CD44. ConclusionsThe observed results collectively underscore the substantial anti-in ammatory and anti-apoptotic potential of all four RAPs. Moreover, their capacity to modulate the expression of cell surface adhesion molecules highlights their potential in inhibiting the formation of kidney stones. Notably, RAP3, boasting the highest carboxyl group content, emerges as the most potent agent in this regard.

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Section: Raps Reduced the Cytotoxicity Of Com Crystals And Alleviated...mentioning

confidence: 99%

Section: Raps Reduced the Cytotoxicity Of Com Crystals And Alleviated...mentioning

confidence: 99%

Carboxymethylated Rhizoma alismatis polysaccharides reduces the risk of calcium oxalate stone formation by reducing cellular inflammation and oxidative stress

Wang,

Liu,

et al. 2024

Urolithiasis

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“…However, many negatively charged molecules, such as hyaluronic acid, osteopontin, CD44, etc. 86,87 are expressed on the surface of the damaged renal epithelial cell membrane. Therefore, the repulsion between the negatively charged crystals and the negatively charged cells increased, 88 which not only inhibited the adhesion of COM crystals on the cell surface but also reduced the damage of the crystals to the cells.…”

Section: Dlp-k 3 Cit or Sdlp-k 3 Cit Synergistically Protects Hk-2 Ce...mentioning

confidence: 99%

Synergistic inhibition of calcium oxalate crystal formation and synergistic protection of HK-2 cells from crystal damage by sulfatedLaminarinpolysaccharide and potassium citrate

Liu

Ouyang

2023

Biomater. Sci.

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“…Our previous studies have indicated that PYP can reduce cellular damage from oxalate and nano-CaOx monohydrate crystals. − However, further research is required to determine the detailed molecular mechanism by which PYP inhibits the formation of CaOx kidney stones and its potential role in preventing and treating stone formation in animals. In this study, we will construct a cellular injury and rat crystallization model by high oxalate, and investigate the specific molecular mechanisms by which PYP inhibits CaOx stones in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of Porphyra Yezoensis Polysaccharides in Inhibiting Hyperoxalate-Induced Renal Injury and Crystal Deposition

Deng,

Li,

Huang

et al. 2024

J. Agric. Food Chem.

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Oxidative damage to the kidneys is a primary factor in the occurrence of kidney stones. This study explores the inhibitory effect of Porphyra yezoensis polysaccharides (PYP) on oxalate-induced renal injury by detecting levels of oxidative damage, expression of adhesion molecules, and damage to intracellular organelles and revealed the molecular mechanism by molecular biology methods. Additionally, we validated the role of PYP in vivo using a crystallization model of hyperoxalate-induced rats. PYP effectively scavenged the overproduction of reactive oxygen species (ROS) in HK-2 cells, inhibited the adhesion of calcium oxalate (CaOx) crystals on the cell surface, unblocked the cell cycle, restored the depolarization of the mitochondrial membrane potential, and inhibited cell death. PYP upregulated the expression of antioxidant proteins, including Nrf2, HO-1, SOD, and CAT, while decreasing the expression of Keap-1, thereby activating the Keap1/Nrf2 signaling pathway. PYP inhibited CaOx deposition in renal tubules in the rat crystallization model, significantly reduced high oxalate-induced renal injury, decreased the levels of the cell surface adhesion proteins, improved renal function in rats, and ultimately inhibited the formation of kidney stones. Therefore, PYP, which has crystallization inhibition and antioxidant properties, may be a therapeutic option for the treatment of kidney stones.

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Porphyra yezoensis polysaccharide and potassium citrate synergistically inhibit calcium oxalate crystallization induced by renal epithelial cells and cytotoxicity of the formed crystals

Cited by 16 publications

References 54 publications

Carboxymethylated Rhizoma alismatis polysaccharides reduces the risk of calcium oxalate stone formation by reducing cellular inflammation and oxidative stress

Carboxymethylated Rhizoma alismatis polysaccharides reduces the risk of calcium oxalate stone formation by reducing cellular inflammation and oxidative stress

Synergistic inhibition of calcium oxalate crystal formation and synergistic protection of HK-2 cells from crystal damage by sulfatedLaminarinpolysaccharide and potassium citrate

Mechanism of Porphyra Yezoensis Polysaccharides in Inhibiting Hyperoxalate-Induced Renal Injury and Crystal Deposition

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