Low concentrations of oxazepam induce feeding and molecular changes in Radix balthica juveniles

Lebreton, Morgane; Sire, Sacha; Carayon, Jean-Luc; Malgouyres, Jean-Michel; Vignet, Caroline; Géret, Florence; Bonnafé, Elsa

doi:10.1016/j.aquatox.2020.105694

Cited by 14 publications

(4 citation statements)

References 71 publications

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“…Microtubules are improbable candidates, since after meiosis, their number and length gradually diminish [47]. Whatever the target site is, benzodiazepines alter folliculogenesis and affect meiosis progression, eventually reducing egg quality in mammals [48] or egg number, as reported in the gastropod Radix balthica [20]. In the sea urchin, at fertilization, DLZ can directly affect microtubule formation, causing atypical asters [40] or blocking the calcium-dependent exocytosis of cortical granules [49].…”

Section: Discussionmentioning

confidence: 99%

“…Benzodiazepines also interfere with reproduction, affecting not only reproductive behavior, but also male and female gametes [15,16]. These effects have been described, for example, in bony fish [17], crustaceans (Daphnia [18]; Mysidopsis [19]), and gastropods (Radix balthica [20]). In the embryonic stages, DLZ is embryotoxic, teratogenic, and epigenotoxic, and dysmorphogenesis is accompanied by relevant changes in glycoconjugate distribution [6,14].…”

Section: Introductionmentioning

confidence: 99%

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Benzodiazepine Interference with Fertility and Embryo Development: A Preliminary Survey in the Sea Urchin Paracentrotus lividus

Fogliano,

Carotenuto,

Cirino

et al. 2024

IJMS

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Psychotropic drugs and benzodiazepines are nowadays among the primary substances of abuse. This results in a large and constant release into aquatic environments where they have potentially harmful effects on non-target organisms and, eventually, human health. In the last decades, evidence has been collected on the possible interference of benzodiazepines with reproductive processes, but data are few and incomplete. In this study, the possible negative influence of delorazepam on fertilization and embryo development has been tested in Paracentrotus lividus, a key model organism in studies of reproduction and embryonic development. Sperm, eggs, or fertilized eggs have been exposed to delorazepam at three concentrations: 1 μg/L (environmentally realistic), 5 μg/L, and 10 μg/L. Results indicate that delorazepam reduces the fertilizing capacity of male and female gametes and interferes with fertilization and embryo development. Exposure causes anatomical anomalies in plutei, accelerates/delays development, and alters the presence and distribution of glycoconjugates such as N-Acetyl-glucosamine, α-linked fucose, and α-linked mannose in both morulae and plutei. These results should attract attention to the reproductive fitness of aquatic species exposed to benzodiazepines and pave the way for further investigation of the effects they may exert on human fertility. The presence of benzodiazepines in the aquatic environment raises concerns about the reproductive well-being of aquatic species. Additionally, it prompts worries regarding potential impacts on human fertility due to the excessive use of anxiolytics.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Benzodiazepine Interference with Fertility and Embryo Development: A Preliminary Survey in the Sea Urchin Paracentrotus lividus

Fogliano,

Carotenuto,

Cirino

et al. 2024

IJMS

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“…Further investigations are required to fully understand this response, which, in the wild, will severely impair snail survival. In particular, it will be interesting to explain why in this species, as in Radix balthica [18], the benzodiazepine reduces feeding, while in Lymnaea stagnalis [61,62], the effect is exactly the opposite. The neurotransmitter GABA is certainly implicated [63,64], but the way of action remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Concentrations from nanograms to several micrograms have been reported in surface waters [10,11], a range in which measurable effects on target organisms can actually be exerted: GABA-A receptors are evolutionarily conserved across the animal kingdom [16], and their improper activation induces relevant behavioral alterations. Marked depression of motor activity is registered, for example, in Hydra, Daphnia, and Dugesia [17] or Radix balthica [18]. However, exposure is also reported to stimulate locomotor performance, a paradoxical effect reported, for example, in Danio rerio [19].…”

Section: Introductionmentioning

confidence: 99%

Benzodiazepine Delorazepam Induces Locomotory Hyperactivity and Alterations in Pedal Mucus Texture in the Freshwater Gastropod Planorbarius corneus

Fogliano,

Carotenuto,

Agnisola

et al. 2023

IJMS

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Benzodiazepines, psychotropic drugs, are ubiquitous in the aquatic environment due to over-consumption and inefficient removal by sewage treatment plants. Bioaccumulation with consequent behavioral and physiological effects has been reported in many aquatic species. However, the responses are species-specific and still poorly understood. To improve the knowledge, we exposed the freshwater snail Planorbarius corneus to 1, 5, or 10 µg/L of delorazepam, the most widely consumed benzodiazepine in Italy. Conventional behavioral tests were used to assess the effects on locomotor and feeding behavior. Histological and biochemical analyses were also performed to detect possible changes in the structure and composition of the foot mucus and glands. The results show a paradoxical response with reduced feeding activity and locomotor hyperactivity. Pedal mucus was altered in texture but not in composition, becoming particularly rich in fibrous collagen-like material, and a significant change in the protein composition was highlighted in the foot. In conclusion, exposure to delorazepam induces disinhibited behavior in Planorbarius corneus, potentially increasing the risk of predation, and an increase in mucus protein production, which, together with reduced feeding activity, would severely compromise energy resources.

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Tracking drugged waters from various sources to drinking water—its persistence, environmental risk assessment, and removal techniques

Phukan

Kumar

2023

Environ Sci Pollut Res

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Low concentrations of oxazepam induce feeding and molecular changes in Radix balthica juveniles

Cited by 14 publications

References 71 publications

Benzodiazepine Interference with Fertility and Embryo Development: A Preliminary Survey in the Sea Urchin Paracentrotus lividus

Benzodiazepine Interference with Fertility and Embryo Development: A Preliminary Survey in the Sea Urchin Paracentrotus lividus

Benzodiazepine Delorazepam Induces Locomotory Hyperactivity and Alterations in Pedal Mucus Texture in the Freshwater Gastropod Planorbarius corneus

Tracking drugged waters from various sources to drinking water—its persistence, environmental risk assessment, and removal techniques

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