Assessing the cellular toxicity of peptide inhibitors of intracellular protein-protein interactions by microinjection

Spectroscopic features of a perylenediimide probe for sensing amyloid fibrils: in vivo imaging of Aβ-aggregates in a Drosophila model organism

“…In this regard, to assess the diagnostic feature of the L-Phe-PDI dye towards the amyloid fibrils or plaques present in the brain/CNS region and retinal region, we have examined the blood-brainbarrier and the blood-retina-barrier permeability of the L-Phe-PDI in Ab-mutant Drosophila flies after suitably treating them with different doses of L-Phe-PDI. 37,38 For mapping the brain section, intact fly heads of both treated and control flies were dissected out and directly observed under a fluorescence microscope. Fig.…”