The NLRP3 inflammasome drives inflammation in ischemia/reperfusion injury after transient middle cerebral artery occlusion in mice

Franke, Maximilian; Bieber, Michael; Kraft, Peter; Weber, Alexander N.R.; Stoll, Guido; Schuhmann, Michael K.

doi:10.1016/j.bbi.2020.12.009

Cited by 208 publications

(160 citation statements)

References 37 publications

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“…A significant reduction of the BBB disruption was detected with all the aforementioned techniques. The comprehensive details can be found in the original publication [5].…”

Section: Methodsmentioning

confidence: 99%

“…This method was used in the original publication out of which the results were taken for correlation [5]. Dissected cortices and basal ganglia from mouse brains were homogenized with a RIPA buffer (25 mM Tris pH 7.4, 150 mM NaCl, 1% NP-40, 0.1% SDS) containing 4% proteinase inhibitor (cOmplete protease inhibitor cocktail, Sigma Aldrich, Germany) and sonified for 10 s. After centrifugation at 15,000× g for 30 min at 4 • C, supernatants were used for a bicinchoninic acid (BCA) protein assay and subsequent WB analysis.…”

Section: Albumin Western Blotmentioning

confidence: 99%

“…This method was used in the original publication out of which the results were taken for correlation [5]. The first steps until the administration of the blocking buffer were analogous to those of method 3.1.…”

Section: Igg Western Blotmentioning

confidence: 99%

“…This method was used in the original publication out of which the results were taken for correlation [5]. For immunofluorescence staining against albumin, cryo-embedded coronal brain sections (2 mm) were cut into 10-µm-thick slices, fixed with aceton for 15 min and blocked for 60 min in 10% BSA in PBS containing 1% goat serum to prevent unspecific binding.…”

Section: Albumin Immunofluorescence Staining and Intensity Measurementmentioning

confidence: 99%

“…The BBB disruption represents a key pathological feature of ischemic strokes (IS). As a result, endothelial lesions occur, para-cellular and trans-cellular permeability increases, water and ion homeostasis disrupt and infiltrating pro-inflammatory cells, like leukocytes, further exacerbate inflammatory processes and brain injury [1][2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

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Validity and Efficacy of Methods to Define Blood Brain Barrier Integrity in Experimental Ischemic Strokes: A Comparison of Albumin Western Blot, IgG Western Blot and Albumin Immunofluorescence

Franke

Bieber

Stoll

et al. 2021

MPs

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The clinical and preclinical research of ischemic strokes (IS) is becoming increasingly comprehensive, especially with the emerging evidence of complex thrombotic and inflammatory interactions. Within these, the blood brain barrier (BBB) plays an important role in regulating the cellular interactions at the vascular interface and is therefore the object of many IS-related questions. Consequently, valid, economic and responsible methods to define BBB integrity are necessary. Therefore, we compared the three ex-vivo setups albumin Western blot (WB), IgG WB and albumin intensity measurement (AIM) with regard to validity as well as temporal and economic efficacy. While the informative value of the three methods correlated significantly, the efficacy of the IgG WB dominated.

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“…A significant reduction of the BBB disruption was detected with all the aforementioned techniques. The comprehensive details can be found in the original publication [5].…”

Section: Methodsmentioning

confidence: 99%

Section: Albumin Western Blotmentioning

confidence: 99%

Section: Igg Western Blotmentioning

confidence: 99%

Section: Albumin Immunofluorescence Staining and Intensity Measurementmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Validity and Efficacy of Methods to Define Blood Brain Barrier Integrity in Experimental Ischemic Strokes: A Comparison of Albumin Western Blot, IgG Western Blot and Albumin Immunofluorescence

Franke

Bieber

Stoll

et al. 2021

MPs

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Edaravone dexborneol alleviates cerebral ischemia‐reperfusion injury through NF‐κB/NLRP3 signal pathway

Shen

Lou

et al. 2023

The Anatomical Record

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Inflammatory injury following ischemia‐reperfusion (I/R) severely limits the efficacy of stroke treatment. Edaravone dexborneol (C.EDA) has been shown to reduce inflammation following a cerebral hemorrhage. However, the precise anti‐inflammatory mechanism of C.EDA is unknown. In this study, we investigated whether C.EDA provides neuroprotection after I/R in rats, as well as the potential mechanisms involved. A middle cerebral artery occlusion/reperfusion (I/R) model was created using Sprague‐Dawley rats. The blood flow of the central cerebral artery was monitored by a laser speckle imaging system. The neurological score was used to assess behavioral improvement. Cerebral infarction volume was measured by TTC staining. And the integrity of the blood–brain barrier was detected by Evan's blue staining. The expression of the nuclear factor kappa‐B (NF‐κB)/ the NOD‐like receptor protein (NLRP3) inflammasome signal pathway and microglia polarization were detected by immunofluorescence and Western blotting. The cerebral blood flow ratio indicates that the cerebral I/R model was successfully established. After reperfusion for 72 h, the improvement of neurological scores, infarct volume reduction, and integrity of the blood–brain barrier was observed in I/R rats with C.EDA treatment. Meanwhile, the immunofluorescence result showed that the expression of iNOS, NLRP3, and NF‐κB protein was decreased and the level of Arg1 was increased. Western blot analysis showed that the expression of NF‐κB/NLRP3 signal pathway‐related protein was decreased. In conclusion, this study indicates that C.EDA alleviates I/R injury by blocking the activation of the NLRP3 inflammasome and regulating the polarization of M1/M2 microglia via the NF‐κB signal pathway.

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Sodium Danshensu ameliorates cerebral ischemia/reperfusion injury by inhibiting CLIC4/NLRP3 inflammasome‐mediated endothelial cell pyroptosis

et al. 2023

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Endothelial pyroptosis promotes cerebral ischemia/reperfusion injury (CIRI). Sodium Danshensu (SDSS) has been shown to attenuate CIRI and have anti‐inflammatory properties in endothelial cells. However, the mechanism and effect of SDSS on alleviating endothelial pyroptosis after CIRI remains poorly understood. Thus, we aimed to investigate the efficacy and mechanism of SDSS in reducing endothelial pyroptosis. It has been shown that SDSS administration inhibited NLRP3 inflammasome‐mediated pyroptosis. As demonstrated by protein microarrays, molecular docking, CETSA and ITDRFCETSA, SDSS bound strongly to CLIC4. Furthermore, SDSS can decrease its expression and inhibit its translocation. Its effectiveness was lowered by CLIC4 overexpression but not by knockdown. Overall The beneficial effect of SDSS against CIRI in this study can be ascribed to blocking endothelial pyroptosis by binding to CLIC4 and then inhibiting chloride efflux‐dependent NLRP3 inflammasome activation.

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The NLRP3 inflammasome drives inflammation in ischemia/reperfusion injury after transient middle cerebral artery occlusion in mice

Cited by 208 publications

References 37 publications

Validity and Efficacy of Methods to Define Blood Brain Barrier Integrity in Experimental Ischemic Strokes: A Comparison of Albumin Western Blot, IgG Western Blot and Albumin Immunofluorescence

Validity and Efficacy of Methods to Define Blood Brain Barrier Integrity in Experimental Ischemic Strokes: A Comparison of Albumin Western Blot, IgG Western Blot and Albumin Immunofluorescence

Edaravone dexborneol alleviates cerebral ischemia‐reperfusion injury through NF‐κB/NLRP3 signal pathway

Sodium Danshensu ameliorates cerebral ischemia/reperfusion injury by inhibiting CLIC4/NLRP3 inflammasome‐mediated endothelial cell pyroptosis

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