Feed-additive of bioengineering strain with surface-displayed laccase degrades sulfadiazine in broiler manure and maintains intestinal flora structure

Li, Rong; Zhou, Tuoyu; Khan, Aman; Ling, Zhenmin; Sharma, Monika; Feng, Ping; Ali, Gohar; Saif, Irfan; Wang, Haoyang; Li, Xiangkai; Liu, Pu

doi:10.1016/j.jhazmat.2020.124440

Cited by 19 publications

(18 citation statements)

References 45 publications

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“…The degradation of sulfadiazine by using the whole-cell biocatalyst EcN revealed the differences between EcN-Lacc6 and EcN-IL, in which the surface exhibited a higher efficiency of EcN-IL under 30, 50, and 100 mg L −1 sulfadiazine. 15 The Se(IV) reduction rate of surface-engineered EcN-IS was about 10% higher than that of EcN expressing plasmids in the cytoplasm (Fig. 2b).…”

Section: Discussionmentioning

confidence: 90%

“…13 Functional enzymes displayed on the cell surface of probiotic bacteria could enhance their functional capacity (e.g., laccase, tetracycline-degrading enzyme TetX, and metal regulatory protein CueR). 14,15 In this study, the selenite reductase protein SerV01 was identified from Staphylococcus aureus LZ-01. SerV01 was displayed on the cell surface of EcN using cell surface display technology, and the reduction performance of the engineered strain EcN-IS to Se(IV) was also studied.…”

Section: Introductionmentioning

confidence: 95%

“…On the whole, 60 14-day-old male local broilers (Xigu Poultry Farming Company, Lanzhou, China) were weighed, then grouped and raised in mounted wire fences. 15 The product standard number of chicken feed is Q/NBCD 01-2020 (Zhengda Agriculture Co., Ltd), which passed the certification of the International Organization for Standardization (ISO) 9001 quality management system (Table S1 †). The body weight and the feed consumption per pen were recorded weekly.…”

Section: Broiler Diets and Experimental Designmentioning

confidence: 99%

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Improving selenium accumulation in broilers using Escherichia coli Nissle 1917 with surface-displayed selenite reductase SerV01

Gong

Khan

et al. 2022

Food Funct.

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Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 95%

Section: Broiler Diets and Experimental Designmentioning

confidence: 99%

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Improving selenium accumulation in broilers using Escherichia coli Nissle 1917 with surface-displayed selenite reductase SerV01

Gong

Khan

et al. 2022

Food Funct.

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“…Then, 0.15 µg of engineered bacterial cells was harvested according to the above proportion (total weight of whole cell biocatalyst after centrifugation by 1 mL cell culture). To make freeze-dried powder of the whole cell biocatalyst, a method was referenced based on Li et al reported previously [ 34 ]. The amount of p NP released was determined at 405 nm continuously, and one enzymatic activity unit (U) was regarded as the amount of enzyme required to release 1 µM of p NP per minute.…”

Section: Methodsmentioning

confidence: 99%

“…Localization of target proteins on the cell surface could be used as a whole-cell biocatalyst directly because the substrates do not need to be transported across the membrane into the cells [ 6 ]. Surface-displayed systems developed using E. coli as a host and the N-terminal of ice nucleation protein (INPN) from Pseudomonas syringae as an anchor have been successfully applied in environmental bioremediation, such as biodegradation of phthalic acid esters [ 31 ], pesticides [ 32 ], antibiotics [ 33 , 34 ], and absorption of mercury ions/lead [ 35 , 36 ]. To date, no report using a surface-displayed strategy for carbamate biodegradation has been published.…”

Section: Introductionmentioning

confidence: 99%

Display of a novel carboxylesterase CarCby on Escherichia coli cell surface for carbaryl pesticide bioremediation

et al. 2022

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Background Carbamate pesticides have been widely used in agricultural and forestry pest control. The large-scale use of carbamates has caused severe toxicity in various systems because of their toxic environmental residues. Carbaryl is a representative carbamate pesticide and hydrolase/carboxylesterase is the initial and critical enzyme for its degradation. Whole-cell biocatalysts have become a powerful tool for environmental bioremediation. Here, a whole cell biocatalyst was constructed by displaying a novel carboxylesterase/hydrolase on the surface of Escherichia coli cells for carbaryl bioremediation. Results The carCby gene, encoding a protein with carbaryl hydrolysis activity was cloned and characterized. Subsequently, CarCby was displayed on the outer membrane of E. coli BL21(DE3) cells using the N-terminus of ice nucleation protein as an anchor. The surface localization of CarCby was confirmed by SDS–PAGE and fluorescence microscopy. The optimal temperature and pH of the engineered E. coli cells were 30 °C and 7.5, respectively, using pNPC4 as a substrate. The whole cell biocatalyst exhibited better stability and maintained approximately 8-fold higher specific enzymatic activity than purified CarCby when incubated at 30 °C for 120 h. In addition, ~ 100% and 50% of the original activity was retained when incubated with the whole cell biocatalyst at 4 ℃ and 30 °C for 35 days, respectively. However, the purified CarCby lost almost 100% of its activity when incubated at 30 °C for 134 h or 37 °C for 96 h, respectively. Finally, approximately 30 mg/L of carbaryl was hydrolyzed by 200 U of the engineered E. coli cells in 12 h. Conclusions Here, a carbaryl hydrolase-containing surface-displayed system was first constructed, and the whole cell biocatalyst displayed better stability and maintained its catalytic activity. This surface-displayed strategy provides a new solution for the cost-efficient bioremediation of carbaryl and could also have the potential to be used to treat other carbamates in environmental bioremediation.

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Dual‐emission ratiometric fluorescence sensors based on silica, molecularly imprinted polymers and carbon quantum dots for high‐sensitivity and high‐selectivity detection of sulfadiazine

Yang,

Kuai,

et al. 2023

Polymer International

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In this work, a dual‐emission ratiometric fluorescence sensor was prepared and is used for the detection of sulfadiazine drug. In this paper, the hydrothermal method was used to synthesize CQD (fluorescence response signal) and sulfhydryl modified cadmium telluride quantum dots (fluorescence reference signal), and the molecularly imprinted polymer is synthesized following. After eluting the template molecule SDZ, the blot recognition site remains on the surface of the imprinted polymer. During the process of detection, due to electron transfer between SDZ and CQDs, holes of stimulated electrons are filled. It leads fluorescence quenching around 430 nm, which greatly improves sensor selection performance. Compared with traditional single‐emission fluorescence sensors, ratiometric fluorescence sensors have a wider linear range and higher detection accuracy. Under the optimal conditions, the sensor exhibited excellent performance in terms of low detection limit (4.2 nmol L−1) and its satisfactory linear range (2–20 μmol L−1). This intelligent response change mode also provide prospect for other sulfonamide antibiotics detection and offer a reference to signal transform for other molecular imprinting technology.This article is protected by copyright. All rights reserved.

show abstract

Feed-additive of bioengineering strain with surface-displayed laccase degrades sulfadiazine in broiler manure and maintains intestinal flora structure

Cited by 19 publications

References 45 publications

Improving selenium accumulation in broilers using Escherichia coli Nissle 1917 with surface-displayed selenite reductase SerV01

Improving selenium accumulation in broilers using Escherichia coli Nissle 1917 with surface-displayed selenite reductase SerV01

Display of a novel carboxylesterase CarCby on Escherichia coli cell surface for carbaryl pesticide bioremediation

Dual‐emission ratiometric fluorescence sensors based on silica, molecularly imprinted polymers and carbon quantum dots for high‐sensitivity and high‐selectivity detection of sulfadiazine

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