Ionizing-radiation-resistant Kocuria rhizophila PT10 isolated from the Tunisian Sahara xerophyte Panicum turgidum: Polyphasic characterization and proteogenomic arsenal

Guesmi, Sihem; Pujic, Petar; Nouioui, Imen; Dubost, Audrey; Najjari, Afef; Ghedira, Kaïs; Igual, José Mariano; Miotello, Guylaine; Chérif, Ameur; Armengaud, Jean; Klenk, Hans Peter; Normand, Phillippe; Sghaier, Haïtham

doi:10.1016/j.ygeno.2020.11.029

Cited by 14 publications

(7 citation statements)

References 104 publications

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“…In previous reports, in the genus Planococcus, the strains Planococcus citreus DSM 20549, Planococcus maritimus JCM 11543, and Planococcus dechangensis DSM 25,871 were strongly radiation-resistant bacteria [38,80,81]. In the genus Kocuria, several strains from the species Kocuria rhizophila and Kocuria rosea were reported as radiation-resistant [82][83][84].…”

Section: The Traits Of the Strongly Radiation-resistant Bacteria In T...mentioning

confidence: 91%

High Proportions of Radiation-Resistant Strains in Culturable Bacteria from the Taklimakan Desert

Liu

Chen

et al. 2022

Biology

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The Taklimakan Desert located in China is the second-largest shifting sand desert in the world and is known for its harsh conditions. Types of γ-rays or UV radiation-resistant bacterial strains have been isolated from this desert. However, there is no information regarding the proportions of the radiation-resistant strains in the total culturable microbes. We isolated 352 bacterial strains from nine sites across the Taklimakan Desert from north to south. They belong to Actinobacteria, Firmicutes, Proteobacteria, and Bacteroidetes. The phylum Actinobacteria was the most predominant in abundance and Firmicutes had the highest species richness. Bacteroidetes had the lowest abundance and was found in four sites only, while the other three phyla were found in every site but with different distribution profiles. After irradiating with 1000 J/m2 and 6000 J/m2 UV-C, the strains with survival rates higher than 10% occupied 72.3% and 36.9% of all culturable bacteria, respectively. The members from Proteobacteria had the highest proportions, with survival rates higher than 10%. After radiation with 10 kGy γ-rays, Kocuria sp. TKL1057 and Planococcus sp. TKL1152 showed higher radiation-resistant capabilities than Deinococcus radiodurans R1. Besides obtaining several radiation-resistant extremophiles, this study measured the proportions of the radiation-resistant strains in the total culturable microbes for the first time. This study may help to better understand the origin of radioresistance, especially by quantitatively comparing proportions of radiation-resistant extremophiles from different environments in the future.

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Section: The Traits Of the Strongly Radiation-resistant Bacteria In T...mentioning

confidence: 91%

High Proportions of Radiation-Resistant Strains in Culturable Bacteria from the Taklimakan Desert

Liu

Chen

et al. 2022

Biology

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“…The resistance to gamma radiation is often found in the genus Kocuria which was substantiated by studies, with Guesmi et al (2021) reporting a D10 value of 2.9 kGy for K . rhizophila PT10 strain [ 72 ], and Asgarani et al (2012) determining a D10 value of 2 kGy for K . rosea ASB-107 [ 62 ].…”

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of multiple-stress tolerant bacteria from radon springs

Timkina,

Kulišová,

Palyzová

et al. 2024

PLoS ONE

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Radon springs, characterized by their high concentrations of radon gas (Rn222), are extreme environments with unique physicochemical conditions distinct from conventional aquatic ecosystems. Our research aimed to investigate microbial life in radon springs, focusing on isolating extremophilic bacteria and assessing their resistance to adverse conditions. Our study revealed the prevalence of Actinomycetia species in the radon spring environment. We conducted various tests to evaluate the resistance of these isolates to oxidative stress, irradiation, desiccation, and metal ion content. These extremophilic bacteria showed overall higher resistance to these stresses compared to control strains. Lipidomic analysis was also employed to provide insights into the adaptive mechanisms of these bacteria which were found mainly in the correlations among individual clusters and changes in content of fatty acids (FA) as well as differences between content and type of FAs of environmental isolates and type strains.

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“…Probably, the higher capacity of these strains to endure harsh conditions, like radiation, might be attributed to the activation or expression of specific proteins in response to radiation-induced stress. The literature provides information about isolates of these species that exhibit increased resistance to radiation stress (48,54) . Further research should focus on determining the microflora using molecular and spectrometric tests and linking it with the occurrence of urinary tract reactions, which may enable the selection of patients who are at higher risk of urinary tract complications after radiotherapy.…”

Section: Discussionmentioning

confidence: 99%

Urine microbiome changes during and after radiotherapy for prostate cancer

Złoch,

Sibińska,

Monedeiro

et al. 2024

Preprint

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Background: The urinary microbiome may play a new important role in the development of complications, but still, there is no information about their changes during and after radiotherapy (RT). This study aimed to use the matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) technique to identify the microbiome and assess its changes in urine samples of 88 patients irradiated for prostate cancer. Material and methods: Blood for biochemical analysis and urine samples for MALDI were collected at various time points before gold fiducial implantation (t1) at the beginning (t2) and end of radiotherapy (t3); during follow-up, 1 (t4), 4 (t5), 7 (t6) months after the end of treatment. Results: We identified 1801 different microbial isolates, in 89% (470/528) samples revealed the presence of at least one microbial species among which 79% (373/470) were polymicrobial. Species level: 136 G+, 29 G-, 2 Candida have been noted. The far most abundant group of the identified microorganisms was Staphylococcus members - 51.6% of all isolates followed by Micrococcus (9.1%), Enterococcus (7.6%), Kocuria (5.6%), Corynebacterium (5.4%), and Streptococcus (2.2%). A lower variety of microorganisms incident was observed at the end of RT. The total number of species (TNS) was 50 at t1, increased up to 61 at t2, and then fell to the initial value of 52 at t3. The increase in biodiversity was noted after radiotherapy t4-68, t5-86, and t6-75 (p<0.05). Changes in the biodiversity of the urinary microbiota were also reflected in the differences in the total number of isolates (TNI) - 261, 281, and 273 for time points t1-t3 compared to the 292, 362, and 332 for time points t4-t6 as well as in the total number of detected genera (TNG) - 25, 29, 23 (t1-t3) and 28, 38, 31 (t4-t6). Actinomyces, Corynebacterium, Staphylococcus, Streptococcus, demonstrated significant correlation with the RT stages. Concerning individual species, only K. rhizophila abundance significantly increased with time (p=0.045). Bacteria incidence was strongly correlated with glucose levels in urine. The same correlation was observed for glucose levels in blood, but in a weak manner. Staphylococcus presence was related to higher tPSA. Conclusion: RT for prostate cancer induces a dynamic response in the urinary microbiome, characterized by an initial reduction in diversity post-RT followed by a subsequent increase. Our findings highlight the significant influence of glucose levels in both urine and blood on the urinary microbiota. These insights contribute to the evolving understanding of the interplay between RT, the urinary microbiome, and patient health, paving the way for more targeted interventions and personalized approaches in prostate cancer treatment.

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Ionizing-radiation-resistant Kocuria rhizophila PT10 isolated from the Tunisian Sahara xerophyte Panicum turgidum: Polyphasic characterization and proteogenomic arsenal

Cited by 14 publications

References 104 publications

High Proportions of Radiation-Resistant Strains in Culturable Bacteria from the Taklimakan Desert

High Proportions of Radiation-Resistant Strains in Culturable Bacteria from the Taklimakan Desert

Isolation and characterization of multiple-stress tolerant bacteria from radon springs

Urine microbiome changes during and after radiotherapy for prostate cancer

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