Detection of mycobacteria in paraffin-embedded Ziehl–Neelsen-Stained tissues using digital pathology

Sua, Luz F.; Bolaños, Jhon Edwar; Maya, Juliana; Sanchez, Aura; Medina, Giovanna Beatriz Kalva; Zúñiga-Restrepo, Valeria; Fernández-Trujillo, Liliana

doi:10.1016/j.tube.2020.102025

Cited by 4 publications

(2 citation statements)

References 6 publications

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“…It should be noted that most of our cases (75%) were positive, which is higher than the findings of previous reports, but this is related to the fact that Ziehl–Neelsen positivity was one of the inclusion criteria of our study. The sensitivity of Ziehl–Neelsen staining for the detection of MT in histological specimens is variable, ranging from 0 to 60% [ 2 , 7 , 32 , 33 ]. The difficulty to detect mycobacterium can be explained by its paucibacillary nature [ 5 ], the CDC points out that “there must be between 5000–10000 bacilli per milliliter of specimen to allow the detection of bacteria in stained smears” [ 34 ], and for each slide at least 10 [ 4 ] bacilli [ 7 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemistry and real-time Polymerase Chain Reaction: importance in the diagnosis of intestinal tuberculosis in a Peruvian population

Arevalo,

Rayme,

Ramírez

et al. 2024

BMC Gastroenterol

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Introduction The diagnosis of intestinal tuberculosis is challenging even nowadays. This study aims to report the positivity rates of new diagnostic methods such as immunohistochemistry and Real-Time Polymerase Chain Reaction in patients with intestinal tuberculosis, as well as describe the pathological and endoscopic features of intestinal tuberculosis in our population. Methods This was a retrospective observational study conducted in patients diagnosed with intestinal tuberculosis, between 2010 to 2023 from the Hospital Nacional Daniel Alcides Carrion and a Private Pathology Center, both located in Peru. Clinical data was obtained, histologic features were independently re-evaluated by three pathologists; and immunohistochemistry and real-time Polymerase Chain Reaction evaluation were performed. The 33 patients with intestinal tuberculosis who fulfilled the inclusion criteria were recruited. Results Immunohistochemistry was positive in 90.9% of cases, while real-time Polymerase Chain Reaction was positive in 38.7%. The ileocecal region was the most affected area (33.3%), and the most frequent endoscopic appearance was an ulcer (63.6%). Most of the granulomas were composed solely of epithelioid histiocytes (75.8%). Crypt architectural disarray was the second most frequent histologic finding (78.8%) after granulomas, but most of them were mild. Conclusion Since immunohistochemistry does not require an intact cell wall, it demonstrates higher sensitivity compared to Ziehl–Neelsen staining. Therefore, it could be helpful for the diagnosis of paucibacillary tuberculosis.

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Section: Discussionmentioning

confidence: 99%

Immunohistochemistry and real-time Polymerase Chain Reaction: importance in the diagnosis of intestinal tuberculosis in a Peruvian population

Arevalo,

Rayme,

Ramírez

et al. 2024

BMC Gastroenterol

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“…The specific fluorescent antibody labeling with laser confocal microscopy has been developed to improve the efficiency of M. tuberculosis detection in lung tissue samples, especially when these bacteria have weak ZN staining ( Erokhina et al, 2019 ). The Pat-Scan program constructed using digital pathology has been developed to detect and quantify the bacteria in the paraffin-embedded ZN-stained tissue, thus being helpful in reducing the diagnostic time ( Sua et al, 2021 ). It is worth noting that SSM can neither distinguish dead and live bacteria, nor distinguish M. tuberculosis from nontuberculosis mycobacteria.…”

Section: Improvement and Optimization Of Current Diagnostic Techniquesmentioning

confidence: 99%

Improved Conventional and New Approaches in the Diagnosis of Tuberculosis

Dong

et al. 2022

Front. Microbiol.

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Tuberculosis (TB) is a life-threatening infectious disease caused by Mycobacterium tuberculosis (M. tuberculosis). Timely diagnosis and effective treatment are essential in the control of TB. Conventional smear microscopy still has low sensitivity and is unable to reveal the drug resistance of this bacterium. The traditional culture-based diagnosis is time-consuming, since usually the results are available after 3–4 weeks. Molecular biology methods fail to differentiate live from dead M. tuberculosis, while diagnostic immunology methods fail to distinguish active from latent TB. In view of these limitations of the existing detection techniques, in addition to the continuous emergence of multidrug-resistant and extensively drug-resistant TB, in recent years there has been an increase in the demand for simple, rapid, accurate and economical point-of-care approaches. This review describes the development, evaluation, and implementation of conventional diagnostic methods for TB and the rapid new approaches for the detection of M. tuberculosis.

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Machine-learning methods for detecting tuberculosis in Ziehl-Neelsen stained slides: A systematic literature review

Tamura,

Llano,

Aristizábal

et al. 2024

Intelligent Systems with Applications

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Detection of mycobacteria in paraffin-embedded Ziehl–Neelsen-Stained tissues using digital pathology

Cited by 4 publications

References 6 publications

Immunohistochemistry and real-time Polymerase Chain Reaction: importance in the diagnosis of intestinal tuberculosis in a Peruvian population

Immunohistochemistry and real-time Polymerase Chain Reaction: importance in the diagnosis of intestinal tuberculosis in a Peruvian population

Improved Conventional and New Approaches in the Diagnosis of Tuberculosis

Machine-learning methods for detecting tuberculosis in Ziehl-Neelsen stained slides: A systematic literature review

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