Cellular uptake of polymeric nanoparticles by bovine cumulus-oocyte complexes and their effect on in vitro developmental competence

Gonçalves, Débora Rodrigues; Leroy, Jo; Hees, Sofie Van; Xhonneux, I; Bols, P.E.J.; Kiekens, Filip; Marei, Waleed F.A.

doi:10.1016/j.ejpb.2020.11.011

Cited by 7 publications

(7 citation statements)

References 58 publications

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“…Co‐incubation of NP with eggs and embryos had no harmful effects. [ 24 , 25 , 43 ] NPOv‐embryos transferred in vivo into rabbit uteri had no adverse morphological effect on the endometrium at implantation [ 44 , 45 ] and there was no effect on the number of offspring or body weight at birth.…”

Section: Discussionmentioning

confidence: 99%

Magnetic‐Assisted Control of Eggs and Embryos via Zona Pellucida‐Linked Nanoparticles

García‐Vázquez,

Garrappa,

Luongo

et al. 2024

Advanced Science

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Eggs and embryo manipulation is an important biotechnological challenge to enable positioning, entrapment, and selection of reproductive cells to advance into a new era of nature‐like assisted reproductive technologies. Oviductin (OVGP1) is an abundant protein in the oviduct that binds reversibly to the zona pellucida, an extracellular matrix that surrounds eggs and embryos. Here, the study reports a new method coupling OVGP1 to magnetic nanoparticles (NP) forming a complex (NPOv). NPOv specifically surrounds eggs and embryos in a reversible manner. Eggs/embryos bound to NPOv can be moved or retained when subjected to a magnetic force, and interestingly only mature‐competent eggs are attracted. This procedure is compatible with normal development following gametes function, in vitro fertilization, embryo development and resulting in the birth of healthy offspring. The results provide in vitro proof‐of‐concept that eggs and embryos can be precisely guided in the absence of physical contact by the use of magnets.

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Section: Discussionmentioning

confidence: 99%

Magnetic‐Assisted Control of Eggs and Embryos via Zona Pellucida‐Linked Nanoparticles

García‐Vázquez,

Garrappa,

Luongo

et al. 2024

Advanced Science

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“…In this context, Barkalina et al (2014c) demonstrated that incubation of spermatozoa with MSNPs had minimal impact on DNA integrity, motility, viability, or acrosome morphology. Additionally, PLGA-PEG polymeric NPs encapsulating fluorescein isothiocyanate (FITC) were shown to be taken up by cumulus-enclosed oocytes in a proof-of-concept study ( Goncalves et al, 2020 ). These findings, along with the fact that MSNPs have been shown to be ideal carriers of genetic material (i.e., siRNA and miRNA) ( Pecot et al, 2011 ; Cha et al, 2017 ), raises the prospect that NPs may be an ideal alternative to electroporation or the use of viral vectors for the transfer of genetic material to gametes.…”

Section: Applications Of Nanoparticles In Male Reproductive Health Medicine and Researchmentioning

confidence: 99%

Biocompatible Nanomaterials as an Emerging Technology in Reproductive Health; a Focus on the Male

et al. 2021

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A growing body of research has confirmed that nanoparticle (NP) systems can enhance delivery of therapeutic and imaging agents as well as prevent potentially damaging systemic exposure to these agents by modifying the kinetics of their release. With a wide choice of NP materials possessing different properties and surface modification options with unique targeting agents, bespoke nanosystems have been developed for applications varying from cancer therapeutics and genetic modification to cell imaging. Although there remain many challenges for the clinical application of nanoparticles, including toxicity within the reproductive system, some of these may be overcome with the recent development of biodegradable nanoparticles that offer increased biocompatibility. In recognition of this potential, this review seeks to present recent NP research with a focus on the exciting possibilities posed by the application of biocompatible nanomaterials within the fields of male reproductive medicine, health, and research.

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“…Thus, the lactic acid produced from PLGA nanoparticles degradation may be used as a metabolic substrate and may support the cellular antioxidant capacity in in vitro cultured COC (Gonçalves et al, 2021).…”

Section: Effects Of Nanoparticles On Cumulus-oocyte Complexes Matured In Vitromentioning

confidence: 99%

“…These authors reported that Mel‐LNC was more effective that nonencapsulated melatonin, probably because this configuration facilitates the release of melatonin throughout the cells. These melatonin‐loaded lipid‐core nanoparticles can trigger an antioxidant cascade that produces radical scavenging products, minimizing oxidative stress through a variety of mechanisms (Reiter et al, 2016) Gonçalves et al (2021) showed that nanoparticles produced using biocompatible and biodegradable poly‐lactic‐co‐glycolic acid (PLGA) can be taken up by cumulus cells and to a less extent by the enclosed oocytes during in vitro maturation of bovine COC. These authors reported that nanoparticles transfer to the oocyte appears to be via cumulus cells and transzonal projections, and that exposure of COC to PLGA nanoparticles increases embryo cleavage and blastocyst rates.…”

Section: Effects Of Nanoparticles On Cumulus‐oocyte Complexes Matured In Vitromentioning

confidence: 99%

“…Lactate is an active metabolite involved not only in metabolism, but also in signal transduction and redox regulation (Philip et al, 2005). Thus, the lactic acid produced from PLGA nanoparticles degradation may be used as a metabolic substrate and may support the cellular antioxidant capacity in in vitro cultured COC (Gonçalves et al, 2021). These authors also emphasized that PLGA nanoparticles have promising applications as carriers for drug or molecule delivery targeting in cumulus cells and oocytes.…”

Section: Effects Of Nanoparticles On Cumulus‐oocyte Complexes Matured In Vitromentioning

confidence: 99%

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Benefits and challenges of nanomaterials in assisted reproductive technologies

Silva

Barroso

Nascimento

et al. 2021

Molecular Reproduction Devel

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Assisted reproductive technology (ART) have contributed to preserve fertility in humans and to increase multiplication of genetically superior animals. Despite being highly practiced worldwide, ART presents some challenges, especially because gametes and embryos are kept in vitro for a variable period of time, and the oxidative stress in vitro can have negative impact on oocyte competence and embryo development. Nanotechnology needs to be considered to help overcome some of those impairments, since it can provide strategies to deliver antioxidants and hormones to gametes and embryos in vitro. The application of nanotechnology to ART can allow the development of new protocols using nanomaterials to improve in vitro oocyte competence and embryo production. This review discusses the applicability of nanomaterials to improve sperm selection, to deliver antioxidants and hormones to preantral follicles, oocytes, and embryos in vitro, as well as the concerns about using nanotechnology in ART.

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Cellular uptake of polymeric nanoparticles by bovine cumulus-oocyte complexes and their effect on in vitro developmental competence

Cited by 7 publications

References 58 publications

Magnetic‐Assisted Control of Eggs and Embryos via Zona Pellucida‐Linked Nanoparticles

Magnetic‐Assisted Control of Eggs and Embryos via Zona Pellucida‐Linked Nanoparticles

Biocompatible Nanomaterials as an Emerging Technology in Reproductive Health; a Focus on the Male

Benefits and challenges of nanomaterials in assisted reproductive technologies

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