Quantitation using HRMS: A new tool for rapid, specific and sensitive determination of catecholamines and deconjugated methanephrines metanephrines in urine

Lefeuvre, Sandrine; Bois-Maublanc, J.; Mongeois, E.; Policarpo, V.; Formaux, L; Francia, T.; Billaud, Éliane; Got, Laurence

doi:10.1016/j.jchromb.2020.122391

Cited by 7 publications

(5 citation statements)

References 37 publications

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“…Neurotransmitters are hard to be detected because they exist at extremely low concentrations in biological samples; therefore, specific and very sensitive bioanalytical methods are required (Bicker et al, 2013). For this reason, solid‐phase microextraction is often used for sample purification and enrichment (Lefeuvre et al, 2021). In this paper, the precolumn derivatization method was adopted.…”

Section: Discussionmentioning

confidence: 99%

Detection of catecholamine metabolites in urine based on ultra‐high‐performance liquid chromatography–tandem mass spectrometry

Zhang

Wang

et al. 2021

Biomedical Chromatography

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The excretion of neurotransmitter metabolites in normal individuals is of great significance for health monitoring. A rapid quantitative method was developed with ultra‐performance liquid chromatography–tandem mass spectrometry. The method was further applied to determine catecholamine metabolites vanilymandelic acid (VMA), methoxy hydroxyphenyl glycol (MHPG), dihydroxy‐phenyl acetic acid (DOPAC), and homovanillic acid (HVA) in the urine. The urine was collected from six healthy volunteers (20–22 years old) for 10 consecutive days. It was precolumn derivatized with dansyl chloride. Subsequently, the sample was analyzed using triple quadrupole mass spectrometry with an electrospray ion in positive and multireaction monitoring modes. The method was sensitive and repeatable with the recoveries 92.7–104.30%, limits of detection (LODs) 0.01–0.05 μg/mL, and coefficients no less than 0.9938. The excretion content of four target compounds in random urine samples was 0.20 ± 0.086 μg/mL (MHPG), 1.27 ± 1.24 μg/mL (VMA), 3.29 ± 1.36 μg/mL (HVA), and 1.13 ± 1.07 μg/mL (DOPAC). In the urine, the content of VMA, the metabolite of norepinephrine and adrenaline, was more than MHPG, and the content of HVA, the metabolite of dopamine, was more than DOPAC. This paper detected the levels of catecholamine metabolites and summarized the characteristics of excretion using random urine samples, which could provide valuable information for clinical practice.

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Section: Discussionmentioning

confidence: 99%

Detection of catecholamine metabolites in urine based on ultra‐high‐performance liquid chromatography–tandem mass spectrometry

Zhang

Wang

et al. 2021

Biomedical Chromatography

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“…Most studies involving absolute quantitation are carried out using targeted assays, most often triple quadrupoles in multiple reaction mode 5 , 6 , 7 , 8 where accurate and robust quantitation can be achieved by choosing selective transitions with optimized instrumental parameters. Absolute quantitation can also be performed through less targeted analyses, using high‐resolution mass spectrometry, 9 , 10 , 11 , 12 such as quadrupole‐time‐of‐flight or orbitrap‐based systems. In these cases, data are acquired in an untargeted manner, and selective data processing allows peaks of interest to be filtered out for further analysis.…”

Section: Introductionmentioning

confidence: 99%

Challenges and recent advances in quantitative mass spectrometry‐based metabolomics

Ghafari,

Sleno

2024

Analytical Science Advances

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The field of metabolomics has gained tremendous interest in recent years. Whether the goal is to discover biomarkers related to certain pathologies or to better understand the impact of a drug or contaminant, numerous studies have demonstrated how crucial it is to understand variations in metabolism. Detailed knowledge of metabolic variabilities can lead to more effective treatments, as well as faster or less invasive diagnostics. Exploratory approaches are often employed in metabolomics, using relative quantitation to look at perturbations between groups of samples. Most metabolomics studies have been based on metabolite profiling using relative quantitation, with very few studies using an approach for absolute quantitation. Using accurate quantitation facilitates the comparison between different studies, as well as enabling longitudinal studies. In this review, we discuss the most widely used techniques for quantitative metabolomics using mass spectrometry (MS). Various aspects will be addressed, such as the use of external and/or internal standards, derivatization techniques, in vivo isotopic labelling, or quantitative MS imaging. The principles, as well as the associated limitations and challenges, will be described for each approach.

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“…The analysis of catecholamines has been performed with reversed‐phase (Bergh et al, 2018) and hydrophilic interaction liquid chromatography (Aturki et al, 2011) or weak cation exchange chromatography in mixed mode (Lefeuvre et al, 2021). In addition to liquid chromatography (LC), gas chromatography (GC) and capillary electrophoresis are also possible separation methods (Tsunoda, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Regarding fluorescence, chemically induced fluorescence (after derivatization with luminol or peroxyoxalate) enhances the fluorescence signal and sensitivity of catecholamines, allowing determination in real samples (Tsunoda, 2006). In addition to these detectors, tandem mass spectrometry coupled to HPLC is a robust option to determine the catecholamine content in real samples, as the mass spectrometry (MS) detector has a high selectivity that allows analytes and matrix interferents to be distinguished based on their masses (Lefeuvre et al, 2021). Extraction protocols used in these methods vary from solid‐phase extraction (SPE) on a cation‐exchange column (Takezawa et al, 2000), C 18 cartridges (Talwar et al, 2002) and aluminum oxide columns (He et al, 1997; Li et al, 2000) to liquid–liquid extraction (Dikunets et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

A LC–MS/MS method for the simultaneous determination of 6‐cyanodopamine, 6‐nitrodopamine, 6‐nitrodopa, 6‐nitroadrenaline and 6‐bromodopamine in human plasma and its clinical application in patients with chronic kidney disease

Ribeiro,

Babadopulos,

de Oliveira

et al. 2024

Biomedical Chromatography

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The aim of this study was to develop a high‐performance liquid chromatography–tandem mass spectrometry method for the determination of 6‐cyanodopamine, 6‐nitrodopamine, 6‐nitrodopa, 6‐nitroadrenaline and 6‐bromodopamine in human plasma samples. Strata‐X 33 μm solid‐phase extraction cartridges were used for the extraction of the catecholamines from human plasma samples. The catecholamines were separated in a 150 × 3 mm Shim‐pack GIST C18‐AQ column with 3 μm particle size, placed in an oven at 40°C and perfused with 82% mobile phase A (acetonitrile–H2O; 90:10, v/v) + 0.4% acetic acid and 18% mobile phase B (deionized H2O) + 0.2% formic acid at a flow rate of 340 μl/min in isocratic mode. The injected volume was 4 μl and the run lasted 4 min. The method was linear from 0.1 to 20 ng/ml and the lower limit of quantification was 0.1 ng/ml for all analytes. The method was applied to evaluate the plasma levels of catecholamines in plasma of patients with chronic kidney disease and allowed the detection for the first time of circulating levels of the novel catecholamines 6‐bromodopamine and 6‐cyanodopamine.

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Quantitation using HRMS: A new tool for rapid, specific and sensitive determination of catecholamines and deconjugated methanephrines metanephrines in urine

Cited by 7 publications

References 37 publications

Detection of catecholamine metabolites in urine based on ultra‐high‐performance liquid chromatography–tandem mass spectrometry

Detection of catecholamine metabolites in urine based on ultra‐high‐performance liquid chromatography–tandem mass spectrometry

Challenges and recent advances in quantitative mass spectrometry‐based metabolomics

A LC–MS/MS method for the simultaneous determination of 6‐cyanodopamine, 6‐nitrodopamine, 6‐nitrodopa, 6‐nitroadrenaline and 6‐bromodopamine in human plasma and its clinical application in patients with chronic kidney disease

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