Farnesol induces mitochondrial/peroxisomal biogenesis and thermogenesis by enhancing the AMPK signaling pathway in vivo and in vitro

Cho, Seon Yeon; Lim, Seona; Ahn, Kwang Seok; Kwak, Hyun Jeong; Park, Jin Bong; Um, Jae‐Young

doi:10.1016/j.phrs.2020.105312

Cited by 9 publications

(12 citation statements)

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“…Protein expression analysis was performed as previously reported 26 . Protein extracts from iWAT and iBAT were prepared by homogenization in radioimmunoprecipitation assay (RIPA) buffer (Cell Signaling Technology, Danvers, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…OCR analysis was performed as previously reported 26 using the MITO‐ID Extracellular O 2 Sensor kit (Enzo Life Science, NY, USA) based on the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

“…Protein expression analysis was performed as previously reported. 26 Protein extracts from iWAT and iBAT were prepared by homogenization in radioimmunoprecipitation assay (RIPA) buffer (Cell Signaling Technology, Danvers, MA, USA). The lysates were resolved by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and transferred onto PVDF membranes.…”

Section: Western Blot Analysismentioning

confidence: 99%

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Ellagic acid induces beige remodeling of white adipose tissue by controlling mitochondrial dynamics and SIRT3

Park

Ahn

et al. 2021

The FASEB Journal

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Section: Methodsmentioning

confidence: 99%

“…OCR analysis was performed as previously reported 26 using the MITO‐ID Extracellular O 2 Sensor kit (Enzo Life Science, NY, USA) based on the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Section: Western Blot Analysismentioning

confidence: 99%

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Ellagic acid induces beige remodeling of white adipose tissue by controlling mitochondrial dynamics and SIRT3

Park

Ahn

et al. 2021

The FASEB Journal

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“…Mitochondrial abundance assay was performed as reported previously [ 21 ]. Briefly, adipocytes were stained with MitoTracker Red reagent for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Fruit of Gardenia jasminoides Induces Mitochondrial Activation and Non-Shivering Thermogenesis through Regulation of PPARγ

et al. 2021

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The extract of the Gardenia jasminoides fruit (GJFE) can been consumed as an herbal tea or used as a yellow dye. Recently, studies report that GFJE exerts inhibitory effects on lipid accumulation and adipogenesis in white adipocytes. We evaluated the thermogenic actions of GJFE by focusing on mitochondrial activation and studying the underlying mechanisms. To investigate the role of GJFE on thermogenesis in mice, we used an acute cold exposure model. After 2 weeks of feeding, the cold tolerance of GJFE-fed mice was notably increased compared to PBS-fed mice. This was due to an increase in thermogenic proteins in the inguinal white adipose tissue of the cold-exposed mice. Moreover, GJFE significantly increased thermogenic factors such as peroxisome proliferator-activated receptor gamma (PPARγ), uncoupling protein 1 (UCP1), and PPARγ coactivator 1 alpha (PGC1α) in vitro as well. Factors related to mitochondrial abundance and functions were also induced by GJFE in white and beige adipocytes. However, the treatment of PPARγ inhibitor abolished the GJFE-induced changes, indicating that activation of PPARγ is critical for the thermogenic effect of GJFE. In conclusion, GJFE induces thermogenic action by activating mitochondrial function via PPARγ activation. Through these findings, we suggest GJFE as a potential anti-obesity agent with a novel mechanism involving thermogenic action in white adipocytes.

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“…Recent studies report that farnesol shows anticancer activity via PI3K/AKT pathway in different cancer cells such as colorectal, cervical, and lung cancer (Cho et al, 2021). In a previous study, it was reported that farnesol inhibited Ras protein and NF-kB activation and induced apoptosis (Jung et al, 2018).…”

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confidence: 99%

The investigation of in vitro effects of farnesol at different cancer cell lines

Öztürk

Feyzullazade

Dag

et al. 2022

Microscopy Res & Technique

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Farnesol (trans, trans-3,7,11-trimethyl-2,6,10-dodecatriene-1-ol) is an essential oil component that can be found in a variety of plants. In this study, in vitro effects of farnesol on human lung cancer A549 cell line, colon adenocarcinoma (Caco-2) cell line and healthy human lung epithelial BEAS-2B cell lines, WST-1 cytotoxicity test, dual staining of cell survival (DAPI-PI) analysis, micronucleus test, and transmission electron microscopy (TEM). Farnesol acted in a concentration-dependent manner at the dose ranges studied for cancer cell lines, and while at certain doses it reduced proliferation, interestingly at higher concentrations it induced growth more than the control. In the healthy BEAS-2B cell line, it was tested over a wide range of doses and at all studied concentrations, it did not suppress cellular growth, but rather increased. This seems promising in that farnesol harms cancer cell lines but does not cause significant damage to healthy cells. Obtained TEM data after treatment with farnesol at IC50 dose showed both autophagic and apoptotic findings in cancer cell lines compared to control, and normal findings exhibited in BEAS-2B cell line, cell survival, and micronucleus analyzes showed the presence of apoptotic findings and chromosomal damage as a result of farnesol application in cancer cell lines. Research Highlights• Farnesol has dose-dependent effects on human lung cancer and colon adenocarcinoma cell lines, with no significant damaging effects on healthy human lung epithelial cell lines.• TEM, cell survival, and micronucleus findings support the findings of autophagic, apoptotic, and chromosomal damage on cancer cell lines.

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Farnesol induces mitochondrial/peroxisomal biogenesis and thermogenesis by enhancing the AMPK signaling pathway in vivo and in vitro

Cited by 9 publications

References 53 publications

Ellagic acid induces beige remodeling of white adipose tissue by controlling mitochondrial dynamics and SIRT3

Ellagic acid induces beige remodeling of white adipose tissue by controlling mitochondrial dynamics and SIRT3

Fruit of Gardenia jasminoides Induces Mitochondrial Activation and Non-Shivering Thermogenesis through Regulation of PPARγ

The investigation of in vitro effects of farnesol at different cancer cell lines

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