Process scale-up of an efficient acid-catalyzed steam pretreatment of rice straw for xylitol production by C. Tropicalis MTCC 6192

Singh, Surender; Kaur, Dalveer; Yadav, Sudesh Kumar; Krishania, Meena

doi:10.1016/j.biortech.2020.124422

Cited by 36 publications

(11 citation statements)

References 30 publications

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“…The major components of 1.5% v/v H 2 SO 4 catalyzed untreated hydrolysate were (gL −1 ): sugars (1.0 ± 0.26 glucose, 14.91 ± 0.53 xylose, and 3.60 ± 0.79 arabinose), aliphatic acids (1.5 ± 0.13 acetic acid, 0.28 ± 0.08 formic acid, and 0.04 ± 0.01 levulinic acid), furans (0.029 ± 0.017 HMF and 0.112 ± 0.002 furfural) and total phenols (0.98 ± 0.007). As previously reported, there was a significant decrease in the amount of these inhibitors after detoxification and evaporation due to vacuum concentration [ 31 ]. However, inhibitors began to accumulate again as RSH concentration increased, with RSH1 having the least amount of total organic acids (1.54 ± 0.10 gL −1 ), furans (0.049 ± 0.004 gL −1 ), total phenols (0.64 ± 0.26 gL −1 ) and RSH3 having the most, i.e., 2.43 ± 0.25 gL −1 , 0.078 ± 0.061 gL −1 and 2.47 ± 0.052 gL −1 , respectively.…”

Section: Resultsmentioning

confidence: 54%

“…RS was procured from local farms in Mohali region of Punjab, India. The RS was subjected to dilute acid catalyzed steam pretreatment using 1.5% v/v H 2 SO 4 for 30 min according to the method proposed by Singh et al [ 31 ] and modified accordingly. After bringing the acidic RSH (rice straw hydrolysate) to pH 3.5 with Ba(OH) 2 , it was centrifuged for 25 min to remove the insoluble salt, Ba(SO 4 ) 2 .…”

Section: Methodsmentioning

confidence: 99%

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Bioprocess optimization for enhanced xylitol synthesis by new isolate Meyerozyma caribbica CP02 using rice straw

Singh,

Arya,

Krishania

2024

Biotechnol Biofuels

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The present work models the fermentation process parameters of the newly isolated, Meyerozyma caribbica CP02 for enhanced xylitol production and its fermentability study on rice straw hydrolysate. The study examined the impact of each of the process variables by one variable at a time optimization followed by statistical validation. Temperature of 32 °C, pH of 3.5, agitation of 200 rpm, 1.5% (v/v) inoculum, 80 gL−1 initial xylose was optimized. Subsequently, a sequential two-stage agitation approach was adopted for fermentation. At these optimized conditions, xylitol yield of 0.77 gg−1 and 0.64 gg−1 was achieved using media containing commercial and rice straw derived xylose, respectively. For scale up, in 3L batch bioreactor, the highest xylitol yield (0.63 gg−1) was attained at 72 h with rice straw hydrolysate media containing initial xylose (59.48 ± 0.82 gL−1) along with inhibitors (1.55 ± 0.10 gL−1 aliphatic acids, 0.0.048 ± 0.11 gL−1 furans, 0.64 ± 0.23 gL−1 total phenols). The results imply that even under circumstances characterized by an acidic pH and elevated initial xylose level, M. caribbica CP02, as an isolate, displays robustness and shows favorable fermentability of rice straw hydrolysate. Therefore, isolate CP02 has potential to be used in bio-refineries for high yield xylitol production with minimal hydrolysate processing requirements. Graphical Abstract

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Section: Resultsmentioning

confidence: 54%

Section: Methodsmentioning

confidence: 99%

Bioprocess optimization for enhanced xylitol synthesis by new isolate Meyerozyma caribbica CP02 using rice straw

Singh,

Arya,

Krishania

2024

Biotechnol Biofuels

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“…Both studies involved dilute sulfuric acid treatments of rice straw. In these investigations, the C. tropicalis strains produced similar xylitol levels and yields on the pretreated rice straw independent of fermentation time at pretreatment process at 30 • C [48,49]. A dilute sulfuric acid-treated sisal fiber (48.2% cellulose, 31.8% hemicellulose and 11.1% lignin) hydrolysate supported a low xylitol yield by C. tropicalis CCT 1516 after 60 h at 30 • C compared to the majority of agricultural residues (Table 1) tested [50].…”

Section: Xylitol Production By Candida Species From Agricultural Residuesmentioning

confidence: 79%

“…Xylitol synthesis by C. guilliermondii ATCC 201935 grown on the rapeseed straw hydrolysate was shown to produce a slightly higher xylitol level than that observed compared to the xylitol level produced by the same C. guilliermondii strain grown on the hydrolysate from apple pomace [33,47]. Two studies analyzed rice straw (25% cellulose, 18% hemicellulose and 21.6% lignin) hydrolysates as substrates for xylitol production by C. tropicalis [48,49]. Both studies involved dilute sulfuric acid treatments of rice straw.…”

Section: Xylitol Production By Candida Species From Agricultural Residuesmentioning

confidence: 99%

Xylitol Production by Candida Species from Hydrolysates of Agricultural Residues and Grasses

West

2021

Fermentation

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Xylitol is an industrially important chemical due to its commercial applications. The use of xylitol as a sweetener as well as its utilization in biomedical applications has made it a high value specialty chemical. Although several species of yeast synthesize xylitol, this review focusses on the species of the genus Candida. The importance of the enzyme xylitol reductase present in Candida species as it relates to their ability to synthesize xylitol was examined. Another focus of this work was to review prior studies examining the ability of the Candida species to synthesize xylitol effectively from hydrolysates of agricultural residues and grasses. An advantage of utilizing such a hydrolysate as a substrate for yeast xylitol production would be decreasing the overall cost of synthesizing xylitol. The intent of this review was to learn if such hydrolysates could substitute for xylose as a substrate for the yeast when producing xylitol. In addition, a comparison of xylitol production by Candida species should indicate which hydrolysate of agricultural residues and grasses would be the best substrate for xylitol production. From studies analyzing previous hydrolysates of agricultural residues and grasses, it was concluded that a hydrolysate of sugarcane bagasse supported the highest level of xylitol by Candida species, although corncob hydrolysates also supported significant yeast xylitol production. It was also concluded that fewer studies examined yeast xylitol production on hydrolysates of grasses and that further research on grasses may provide hydrolysates with a higher xylose content, which could support greater yeast xylitol production.

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“…Rice straw was subjected to acidic pretreatment with sulphuric acid (H 2 SO 4 ) as described by Singh et al 14 ; biomass was pretreated with 1.5% (v/v) diluted H 2 SO 4 (1 : 10 w/v) for 24 h. The pH of pretreated rice straw hydrolysate (RSH) was 0.8; it was neutralized with barium hydroxide till pH 7 was attained and detoxified with 1.5% activated charcoal. The sugar content of hydrolysate before and after pretreatment was evaluated using HPLC (Agilent, HiPlex, Santa Clara, California, USA).…”

Section: Methodsmentioning

confidence: 99%

Sustainable production of prodigiosin from rice straw derived xylose by using isolated Serratia marcescens (CMS 2): statistical optimization, characterization, encapsulation & cost analysis

Miglani,

Singh,

Singh

et al. 2023

Sustainable Food Technol.

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Process scale-up of an efficient acid-catalyzed steam pretreatment of rice straw for xylitol production by C. Tropicalis MTCC 6192

Cited by 36 publications

References 30 publications

Bioprocess optimization for enhanced xylitol synthesis by new isolate Meyerozyma caribbica CP02 using rice straw

Bioprocess optimization for enhanced xylitol synthesis by new isolate Meyerozyma caribbica CP02 using rice straw

Xylitol Production by Candida Species from Hydrolysates of Agricultural Residues and Grasses

Sustainable production of prodigiosin from rice straw derived xylose by using isolated Serratia marcescens (CMS 2): statistical optimization, characterization, encapsulation & cost analysis

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