Transcription factor C/EBPβ induces genome-wide H3K27ac and upregulates gene expression during decidualization of human endometrial stromal cells

Tamura, Isao; Maekawa, Ryo; Jozaki, Kosuke; Ohkawa, Yasuyuki; Takagi, Hajime; Doi-Tanaka, Yumiko; Shirafuta, Yuichiro; Mihara, Yumiko; Tanaka, Toshiaki; Sato, Shun; Tamura, Hiroshi; Sugino, Norihiro

doi:10.1016/j.mce.2020.111085

Cited by 20 publications

(39 citation statements)

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Section: Introductionmentioning

confidence: 94%

“…Acetylation of histone-H3 lysine-27 (H3K27ac) is one of the histone modifications that activates gene transcription and is highly enriched in the active promoter or enhancer regions (19). We found that decidualization stimulus up-regulates a number of gene expressions with the genome-wide increase of H3K27ac levels (4,5,20).…”

Section: Introductionmentioning

confidence: 98%

“…Decidualization is one of these changes in which ESCs differentiate into decidual cells by the action of progesterone, which is essential for implantation and maintenance of pregnancy ( 1 , 2 , 3 ). Our previous genome-wide analyses revealed that a number of genes are up- or downregulated by decidualization in human ESCs ( 4 , 5 ). These changes are accompanied by dramatic metabolic changes including increases in the storage of lipids ( 6 ) and glycogen ( 7 ).…”

mentioning

confidence: 99%

“…In addition, the mechanisms by which GLUT1 is upregulated during decidualization have not been well clarified. Although various transcription factors are activated to upregulate many genes during decidualization ( 4 , 12 , 13 , 14 , 15 ), it remains unclear which transcription factors are involved in the regulation of GLUT1 expression. By performing a transcriptome analysis, we previously revealed that CCAAT enhancer-binding protein β (C/EBPβ) and Wilms’ tumor 1(WT1) are important transcription factors regulating a number of decidualization-related genes ( 4 , 6 ), which led us to focus on these transcription factors as upstream regulators of GLUT1 during decidualization.…”

mentioning

confidence: 99%

“…These findings suggested that H3K27ac is associated with glucose metabolisms. Furthermore, our recent ChIP-sequence analysis indicated that the H3K27ac level of the GLUT1 promoter region increases during decidualization ( 4 ). Therefore, we hypothesized that the change of H3K27ac is an important mechanism for the regulation of GLUT1 and the following glucose uptake during decidualization.…”

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confidence: 99%

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The essential glucose transporter GLUT1 is epigenetically upregulated by C/EBPβ and WT1 during decidualization of the endometrium

Tamura

Fujimura

Doi-Tanaka

et al. 2021

Journal of Biological Chemistry

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Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 98%

mentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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The essential glucose transporter GLUT1 is epigenetically upregulated by C/EBPβ and WT1 during decidualization of the endometrium

Tamura

Fujimura

Doi-Tanaka

et al. 2021

Journal of Biological Chemistry

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Analysis of cell–cell interaction between mural granulosa cells and cumulus granulosa cells during ovulation using single‐cell RNA sequencing data of mouse ovary

Shirafuta,

Tamura,

Shiroshita

et al. 2024

Reprod Medicine & Biology

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PurposeWe investigated the interactions between mural granulosa cells (MGCs) and cumulus granulosa cells (CGCs) during ovulation after the LH surge.MethodsWe performed clustering, pseudotime, and interactome analyses utilizing reported single‐cell RNA sequencing data of mouse ovary at 6 h after eCG‐hCG injection.ResultsClustering analysis classified granulosa cells into two distinct populations, MGCs and CGCs. Pseudotime analysis divided granulosa cells into before and after the LH surge, and further divided them into two branches, the ovulatory MGCs and the ovulatory CGCs. Interactome analysis was performed to identify the interactions between MGCs and CGCs. Twenty‐six interactions were acting from CGCs toward MGCs, involving ovulation and steroidogenesis. Thirty‐six interactions were acting from MGCs toward CGCs, involving hyaluronan synthesis. There were 25 bidirectional interactions, involving the EGFR pathway. In addition, we found three novel interactions: Ephrins–Ephs pathway and Wnt–Lrp6 pathway from CGCs to MGCs, associated with steroidogenesis and lipid transport, respectively, and TGF‐β–TGFBR1 pathway from MGCs to CGCs, associated with hyaluronan synthesis.ConclusionsMGCs and CGCs interact with each other in the preovulatory follicle after the LH surge, and their interactions have roles in corpus luteum formation, oocyte maturation, and follicle rupture.

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High incidence of decidualization failure in infertile women

Tamura,

Doi‐Tanaka,

Takasaki

et al. 2024

Reprod Medicine & Biology

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PurposeDecidualization is an important event for embryo implantation and successful pregnancy. Impaired decidualization leads to implantation failure and miscarriage. However, it is unclear how often decidualization failure occurs in infertile women. By analyzing the endometrium at late‐secretory phase, we investigated the incidence and pathogenesis of decidualization failure among infertile women.MethodsEndometrial dating was performed on the endometria obtained in the late‐secretory phase from 33 infertile women. Endometrial dating of more than 2 days delay was taken as an indication of decidualization failure. The expression of essential transcription factors for decidualization (FOXO1, WT1, and C/EBPβ) was examined by immunohistochemistry.ResultsAmong 32 cases, 20 cases (62.5%) showed decidualization failure. These patients tended to have a history of more frequent miscarriages than those without decidualization failure. The percentage of cells that immunostained positive for the expression of three transcription factors was significantly lower in the patients with decidualization failure than in those without decidualization failure. Serum progesterone levels measured in the mid‐ and late‐secretory phase were not significantly different between the cases with and without decidualization failure.ConclusionsThe incidence of decidualization failure is high in infertile women.

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Transcription factor C/EBPβ induces genome-wide H3K27ac and upregulates gene expression during decidualization of human endometrial stromal cells

Cited by 20 publications

References 61 publications

The essential glucose transporter GLUT1 is epigenetically upregulated by C/EBPβ and WT1 during decidualization of the endometrium

The essential glucose transporter GLUT1 is epigenetically upregulated by C/EBPβ and WT1 during decidualization of the endometrium

Analysis of cell–cell interaction between mural granulosa cells and cumulus granulosa cells during ovulation using single‐cell RNA sequencing data of mouse ovary

High incidence of decidualization failure in infertile women

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