Antibody‐dependent enhancement (ADE) of dengue virus: Identification of the key amino acid that is vital in DENV vaccine research

Cui, Guohui; Si, Lulu; Wang, Ying; Yan, Huijun; Jiang, Lifang

doi:10.1002/jgm.3297

Cited by 10 publications

(11 citation statements)

References 34 publications

(51 reference statements)

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“…It is also likely that tissue failure following dissemination of the virus through the blood can induce cell death and release of intracellular antigens not known as autoantigens, in addition to those already released in the AT for mechanisms like hypoxia and consequent cell death, as we have previously demonstrated [29]. It has also recently been postulated that antibodydependent enhancement (ADE) may exacerbate the COVID-19 disease, as previously demonstrated with dengue [79] and MERS infections [80]. However, clear evidence of ADE as a cause of severe SARS-CoV-2 infection has not yet been demonstrated.…”

Section: Plos Onementioning

confidence: 63%

Influence of obesity on serum levels of SARS-CoV-2-specific antibodies in COVID-19 patients

et al. 2021

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SARS-CoV-2 (Severe Acute Respiratory Syndrome Corona Virus-2), cause of COVID-19 (Coronavirus Disease of 2019), represents a significant risk to people living with pre-existing conditions associated with exacerbated inflammatory responses and consequent dysfunctional immunity. In this paper, we have evaluated the influence of obesity, a condition associated with chronic systemic inflammation, on the secretion of SARS-CoV-2-specific IgG antibodies in the blood of COVID-19 patients. Our hypothesis is that obesity is associated with reduced amounts of specific IgG antibodies. Results have confirmed our hypothesis and have shown that SARS-CoV-2 IgG antibodies are negatively associated with Body Mass Index (BMI) in COVID-19 obese patients, as expected based on the known influence of obesity on humoral immunity. Antibodies in COVID-19 obese patients are also negatively associated with serum levels of pro-inflammatory and metabolic markers of inflammaging and pulmonary inflammation, such as SAA (serum amyloid A protein), CRP (C-reactive protein), and ferritin, but positively associated with NEFA (nonesterified fatty acids). These results altogether could help to identify an inflammatory signature with strong predictive value for immune dysfunction. Inflammatory markers identified may subsequently be targeted to improve humoral immunity in individuals with obesity and in individuals with other chronic inflammatory conditions.

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Section: Plos Onementioning

confidence: 63%

Influence of obesity on serum levels of SARS-CoV-2-specific antibodies in COVID-19 patients

et al. 2021

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“…This was previously reported for DENV and ZIKV. 61 , 62 In the case of SARS-CoV-2, this association was reported for the first time in the article by Zhou et al, where monoclonal cells were isolated from memory B cells, later a group of non-overlapping receptor-binding domain was identified. (RBD) epitopes that were directly associated with ADE and favored the entry of the virus into Raji cells via an Fcg receptor-dependent mechanism.…”

Section: Sars Cov-2 and Ade What Is Known And What Remains To Be Known?mentioning

confidence: 97%

A review: Antibody-dependent enhancement in COVID-19: The not so friendly side of antibodies

Sánchez-Zuno

Matuz-Flores

González-Estévez

et al. 2021

Int J Immunopathol Pharmacol

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The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), represents an unprecedented global public health emergency with economic and social consequences. One of the main concerns in the development of vaccines is the antibody-dependent enhancement phenomenon, better known as ADE. In this review, we provide an overview of SARS-CoV-2 infection as well as the immune response generated by the host. On the bases of this principle, we also describe what is known about the ADE phenomenon in various viral infections and its possible role as a limiting factor in the development of new vaccines and therapeutic strategies.

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“…Cell lines tested to study ADE were selected based on previous studies regarding ADE of ZIKV and DENV infection [23][24][25][26][27]. K562 cells were obtained from ATCC (CCL-243) and were cultured in Iscove's Modified Dulbecco's Medium (IMDM; Lonza, Basel, Switzerland) supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich, St. Louis, MO, USA), 100 U/mL penicillin, and 100 µg/mL streptomycin (Lonza) at 37 • C, 5% CO 2 .…”

Section: Cell Linesmentioning

confidence: 99%

Comparative Analysis of In Vitro Models to Study Antibody-Dependent Enhancement of Zika Virus Infection

Langerak

Mumtaz

Schoenmakers

et al. 2022

Viruses

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During the 2015–2016 outbreak of Zika virus (ZIKV) in the Americas, a previously unknown severe complication of ZIKV infection during pregnancy resulting in birth defects was reported. Since the ZIKV outbreak occurred in regions that were highly endemic for the related dengue virus (DENV), it was speculated that antibody-dependent enhancement (ADE) of a ZIKV infection, caused by the presence of cross-reactive DENV antibodies, could contribute to ZIKV disease severity. Emerging evidence indicates that, while in vitro models can show ADE of ZIKV infection, ADE does not seem to contribute to congenital ZIKV disease severity in humans. However, the role of ADE of ZIKV infection during pregnancy and in vertical ZIKV transmission is not well studied. In this study, we hypothesized that pregnancy may affect the ability of myeloid cells to become infected with ZIKV, potentially through ADE. We first systematically assessed which cell lines and primary cells can be used to study ZIKV ADE in vitro, and we compared the difference in outcomes of (ADE) infection experiments between these cells. Subsequently, we tested the hypothesis that pregnancy may affect the ability of myeloid cells to become infected through ADE, by performing ZIKV ADE assays with primary cells isolated from blood of pregnant women from different trimesters and from age-matched non-pregnant women. We found that ADE of ZIKV infection can be induced in myeloid cell lines U937, THP-1, and K562 as well as in monocyte-derived macrophages from healthy donors. There was no difference in permissiveness for ZIKV infection or ADE potential of ZIKV infection in primary cells of pregnant women compared to non-pregnant women. In conclusion, no increased permissiveness for ZIKV infection and ADE of ZIKV infection was found using in vitro models of primary myeloid cells from pregnant women compared to age-matched non-pregnant women.

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Antibody‐dependent enhancement (ADE) of dengue virus: Identification of the key amino acid that is vital in DENV vaccine research

Cited by 10 publications

References 34 publications

Influence of obesity on serum levels of SARS-CoV-2-specific antibodies in COVID-19 patients

Influence of obesity on serum levels of SARS-CoV-2-specific antibodies in COVID-19 patients

A review: Antibody-dependent enhancement in COVID-19: The not so friendly side of antibodies

Comparative Analysis of In Vitro Models to Study Antibody-Dependent Enhancement of Zika Virus Infection

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