Feeding status alters exploratory and anxiety-like behaviors in zebrafish larvae exposed to quinpirole

Nabinger, Débora Dreher; Altenhofen, Stefani; Peixoto, Júlia Vasconcellos; Silva, Julia Maria Kuhl da; Bonan, Carla Denise

doi:10.1016/j.pnpbp.2020.110179

Cited by 15 publications

(16 citation statements)

References 79 publications

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“…Although these behavioral phenotypes can be evaluated in a wide variety of tests, the general aspects of zebrafish locomotion are usually analyzed in the novel tank or open field tests ( Bridi et al, 2017 ; Nabinger et al, 2020 ; Petersen et al, 2021 ). In adults, novel tank and open tank tests are performed in a rectangular or trapezoid aquarium and the typical zebrafish behavior is to stay at the bottom of the apparatus for the first few minutes of testing, followed by top exploration, a phenomenon called bottom-dwelling ( Kalueff et al, 2013 ).…”

Section: Zebrafish Behavioral Paradigmsmentioning

confidence: 99%

Standardizing Zebrafish Behavioral Paradigms Across Life Stages: An Effort Towards Translational Pharmacology

2022

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Zebrafish is a prominent vertebrate model, with many of its advantages related to its development, life cycle, and translational ability. While a great number of behavioral phenotypes and tasks to evaluate them are available, longitudinal studies across zebrafish life stages are scarce and made challenging because of the differences between protocols and endpoints assessed at each life stage. In this mini review, we highlight the relevance that longitudinal studies could have for neurobehavioral pharmacology using this model. We also present possible strategies to standardize behavior endpoints in domains related to human diseases throughout the life cycle, especially between larvae and adult fish. Furthermore, we discuss the remaining difficulties of these analyses and explore future advances needed to bridge this knowledge gap.

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Section: Zebrafish Behavioral Paradigmsmentioning

confidence: 99%

Standardizing Zebrafish Behavioral Paradigms Across Life Stages: An Effort Towards Translational Pharmacology

2022

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“…Despite a reduced cognitive repertoire in comparison to adults, zebrafish larvae show specific visual-driven cognitive responses. We used previously established and validated protocols ( Pelkowski et al, 2011 ; Nery et al, 2014 , 2017 ; Nabinger et al, 2018 ; Nabinger et al, 2020 ) to measure aversive and optomotor responses in 7 dpf larvae exposed to atrazine and diuron. No significant differences were observed when treated animals were compared to their corresponding controls.…”

Section: Discussionmentioning

confidence: 99%

“…Adult zebrafish has a well-characterized behavioral repertoire ( Kalueff et al, 2013 ) and consistent protocols to evaluate them under experimental conditions ( Nery et al, 2014 , 2017 ; Altenhofen et al, 2017a ; Bridi et al, 2017 ; Nabinger et al, 2018 ; Nabinger et al, 2020 ; Wiprich et al, 2020 ; Gusso et al, 2021 ). This is, however, the first study assessing the acute toxic effects of diuron on the adult zebrafish behavior.…”

Section: Discussionmentioning

confidence: 99%

“…The optomotor response is a visually driven behavior, in which larvae orientate and move their bodies according to the direction of white and black moving stripes (24.5 cm wide and 1.5 cm high) adapted from Creton (2009) ( Nery et al, 2017 ; Nabinger et al, 2020 ). Seven dpf larvae (15 larvae per dish, in triplicate, per group) were placed in a Petri dish over an LCD monitor, in which a sequence of animated images of moving stripes were presented for 10 min, following 2 min of acclimation.…”

Section: Methodsmentioning

confidence: 99%

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Atrazine and Diuron Effects on Survival, Embryo Development, and Behavior in Larvae and Adult Zebrafish

et al. 2022

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Atrazine and Diuron are widely used herbicides. The use of pesticides contaminates the aquatic environment, threatening biodiversity and non-target organisms such as fish. In this study, we investigated the effects of acute exposure for 96 h hours to atrazine and diuron commercial formulations in zebrafish (Danio rerio, wild-type AB) embryos and larvae and adult stages. We observed a significant concentration-dependent survival decrease and hatching delays in animals exposed to both herbicides and in the frequency of malformations compared to the control groups. Morphological defects included cardiac edema, tail reduction, and head malformation. At 7 days post-fertilization (dpf), atrazine exposure resulted in a reduction in the head length at 2, 2.5, and 5 mg/L and increased the ocular distance at 1, 2, 2.5, and 5 mg/L atrazine when compared to controls. At the same age, diuron increased the ocular distance in animals exposed to diuron (1.0 and 1.5 mg/L) and no effects were observed on the head length. We also evaluated a behavioral repertoire in larvae at 7 dpf, and there were no significant differences in distance traveled, mean speed, time in movement, and thigmotaxis for atrazine and diuron when animals were individually placed in a new environment. The cognitive ability of the larvae was tested at 7 dpf for avoidance and optomotor responses, and neither atrazine nor diuron had significant impacts when treated groups were compared to their corresponding controls. Adults’ behavior was evaluated 7 and 8 days after the end of the acute herbicide exposure. Exploration of a new environment and associated anxiety-like parameters, social interaction, and aggressiveness were not altered. Our results highlight the need for further studies on the sublethal effects of both herbicides and the consideration of the effects of commercial formulas vs. isolated active ingredients. It also emphasizes the need to take sublethal effects into consideration when establishing the environmental limits of residues.

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“…Native from Asia, the zebrafish is a teleost that has high genetic and physiological homology with humans (Lieschke and Currie, 2007), by this reason, this species has been used as a research animal model for different lines such as embryology and development (Hao et al, 2013;Keller et al, 2008), oxidative stress (Choi et al, 2010;Marcon et al, 2018), behavior (Abozaid et al, 2020;Nabinger et al, 2021;Reis et al, 2020) and genetic (Nasevicius and Ekker, 2000;Falcão et al, 2021). Moreover, this aquatic animal is a very interesting environmental bioindicator used in toxicology and ecotoxicology research, due to its capacity to simulate the conditions of an animal in its natural ecosystem (Asharani et al, 2008;Park et al, 2020;Valadas et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Ochratoxin A induces behavioral and neurochemical changes in adult zebrafish

Valadas

Sachett

Marcon

et al. 2021

Preprint

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Ochratoxin A (OTA) is a mycotoxin produced by species of filamentous fungi widely found as a contaminant in food and with high toxic potential. Studies have shown that this toxin cause kidney and liver damage, however, data on the effects of exposure to OTA on the central nervous system are still scarce. Zebrafish (Danio rerio) is a teleost often used in translational research due to its physiological, genetic, and behavioral homology with mammals, in addition to being useful as an environmental bioindicator. Thus, this study aimed to investigate the effects of exposure to OTA on behavioral and neurochemical parameters in adult zebrafish. The animals were treated with different doses of OTA (1.38, 2.77, and 5.53 mg/kg) and submitted to behavioral evaluations in the open tank and social interaction tests. Subsequently, they were euthanized, and the brains were used to assess markers associated with oxidative status. In the open tank test OTA induced changes in distance, absolute turn angle, mean speed, and time-freezing. However, no significant effects were observed in the social interaction test. Moreover, OTA also induced alterations in neurochemical parameters with changes in non-protein thiols (NPSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and glutathione reductase (GR). This study showed that OTA can affect neurobiological aspects in zebrafish even at low doses.

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Feeding status alters exploratory and anxiety-like behaviors in zebrafish larvae exposed to quinpirole

Cited by 15 publications

References 79 publications

Standardizing Zebrafish Behavioral Paradigms Across Life Stages: An Effort Towards Translational Pharmacology

Standardizing Zebrafish Behavioral Paradigms Across Life Stages: An Effort Towards Translational Pharmacology

Atrazine and Diuron Effects on Survival, Embryo Development, and Behavior in Larvae and Adult Zebrafish

Ochratoxin A induces behavioral and neurochemical changes in adult zebrafish

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