Ligustilide inhibits the proliferation of non-small cell lung cancer via glycolytic metabolism

Jiang, Xiufeng; Zhao, Wei; Zhu, Feng; Wu, Hongshuai; Ding, Xiao Ping; Bai, Jinmei; Zhang, Xiaoqing; Qian, Meifang

doi:10.1016/j.taap.2020.115336

Cited by 18 publications

(18 citation statements)

References 32 publications

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“…There was a huge difference in the IHS score in VEGFR-2 in tumor tissue between group C+W and M (H = 12:800, P = 0:002), but there was no statistical difference between group C and W (P > 0:05). It was similar to the results of Jiang et al [17], who found that VEGFR-2 also played a key role in tumor formation, indicating that Spiriva combined with Symbicort could effectively reduce the level of VEGFR-2 and promote the immunologic function of NSCLC.…”

Section: Discussionsupporting

confidence: 90%

Random Walk Algorithm-Based Computer Tomography (CT) Image Segmentation Analysis Effect of Spiriva Combined with Symbicort on Immunologic Function of Non-Small-Cell Lung Cancer

Liu

Liang

et al. 2022

Computational and Mathematical Methods in Medicine

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The objective of this research was to explore the effect of the treatment regimen of Spiriva combined with Symbicort on the immune function of non-small-cell lung cancer (NSCLC) based on computed tomography (CT) imaging features. An automatic CT image segmentation algorithm (RW-CT) was constructed based on random walk (RW) and image segmentation technology. The image segmentation algorithm based on the Toboggan method (C-CT) was introduced to compare with the traditional RW algorithm. 60 subjects were divided into four groups: a Chinese combined with Western medicine group (treated with Spiriva combined with Symbicort, group C+W), a Chinese medicine group (treated with Spiriva, group C), a Western medicine group (treated with Symbicort, group W), and a model group for control (group M). The results show that the Dice coefficient of the RW-CT algorithm was obviously larger than that of the C-CT algorithm and the RW algorithm, while the Hausdorff distance (HD) of the RW-CT algorithm was much smaller than that of the other two algorithms ( P < 0.05 ). The proportion of positive cells of hypoxia-inducible factor-1α (HIF-1α) in group C+W was the least (15%-23%), followed by the group W (21%-29%) and the group C (28%-37%), and that in the group M was the highest (39%-49%). There was a remarkable difference in the immunohistochemical scores (HIS) of vascular endothelial growth factor (VEGF) in the tumor tissues between group C+W and the group M ( H = 14.200 , P = 0.001 ), but there was no great difference from the group C and the group W ( P > 0.05 ). There was a notable difference in the IHS of vascular endothelial factor recepto-2 (VEGFR-2) between the group C+W medication group and the group M ( H = 12.800 , P = 0.002 ), and there was no statistical difference between the group C and W ( P > 0.05 ). In short, the RW-CT constructed based on RW was better than the traditional algorithms for CT image segmentation. The Spiriva combined with Symbicort could effectively inhibit the expression of VEGF, VEGFR-2, and HIF-1α in NSCLC and promote the immunologic function of the body.

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Section: Discussionsupporting

confidence: 90%

Random Walk Algorithm-Based Computer Tomography (CT) Image Segmentation Analysis Effect of Spiriva Combined with Symbicort on Immunologic Function of Non-Small-Cell Lung Cancer

Liu

Liang

et al. 2022

Computational and Mathematical Methods in Medicine

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“…17 Similarly, the suppressive function of ligustilide in cell proliferation has been confirmed in non-small cell lung cancer. 25 It is worth mentioning that ligustilide exhibited little cytotoxicity to normal hepatocytes before we sought to investigate its function in HCC. Of interest, ligustilide restrained cell proliferation and migration but enhanced cell apoptosis in HCC cells.…”

Section: Discussionmentioning

confidence: 99%

Ligustilide counteracts carcinogenesis and hepatocellular carcinoma cell-evoked macrophage M2 polarization by regulating yes-associated protein-mediated interleukin-6 secretion

Yang

Xing

2021

Exp Biol Med (Maywood)

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Cross-communication between cancer cells and macrophages within the tumor microenvironment fulfills the critical roles in the progression of cancers, including hepatocellular carcinoma (HCC). Ligustilide exerts anti-inflammation, anti-injury, and anti-tumor pleiotropic pharmacological functions. Nevertheless, its roles in HCC cells and tumor microenvironment remain elusive. In the current study, ligustilide dramatically restrained HCC cell viability and migration but had little cytotoxicity to normal hepatocytes. Importantly, ligustilide antagonized HCC cell co-culture-induced macrophage recruitment and M2 polarization by enhancing the percentage of CD14+CD206+ cells and macrophage M2 markers (CD163, Arg1, CD206, CCL22, IL-10, and TGF-β). Mechanistically, ligustilide repressed yes-associated protein (YAP) activation by reducing nuclear translocation, protein expression, transcriptional regulatory activity of YAP, and increasing p-YAP levels. Noticeably, blocking the YAP offset the suppressive effects of ligustilide on macrophage recruitment and M2 polarization evoked by HCC cells. Moreover, the release of interleukin-6 (IL-6) was mitigated by ligustilide in a YAP-dependent manner in HCC cells, concomitant with inhibition of IL-6R/STAT3 signaling activation. Of interest, interdicting the IL-6 aggravated ligustilide-mediated suppression in HCC-induced macrophage recruitment and M2 polarization; whereas exogenous IL-6 treatment reversed the above effects. Additionally, blockage of IL-6R signaling also overturned IL-6-induced macrophage recruitment and M2 phenotype. Consequently, these findings support a notion that ligustilide not only restrains HCC cell malignancy but also antagonizes HCC cell-evoked macrophage recruitment and M2 polarization by inhibiting YAP/IL-6 release-induced activation of the IL-6 receptor/signal transducer and activator of transcription 3 (IL-6R/STAT3) signaling. Thus, ligustilide may be a promising therapeutic agent to fight HCC by regulating cancer cells and cross-talk between tumor cells and macrophages in tumor microenvironment.

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“…[7] Antiplatelet activity from a brominated 3-substituted phthalide derivative (5), [42] 3-nbutylphthalide (NBP) (6) which is isolated from the seeds of Apium graveolens as a promising therapeutic agent for ischemic stroke. [43][44][45] Antinociceptive, anti-inflammatory, and anti-proliferative activities were observed with ligustilide (7) [24,28,46,47] which is a major bioactive component of Angelica sinensis. And mild cytotoxic activity (IC 50 : 13.5 μM) on Hep-2 (human larynx carcinoma) cell line from Alcyopterosin E (8) which is isolated from the subAntarctic soft coral Alcyonium paessleri [48] (Figure 2).…”

Section: Introductionmentioning

confidence: 99%

An Overview of Natural and Synthetic Phthalides Involved in Cancer Studies: Past, Present, and Future

et al. 2022

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Phthalides, mainly isolated from two genera: Ligusticum and Angelica of the Apiceae (syn Umbelliferae) family, are thought to be a potent natural compound with many therapeutic effects, such as antiplatelet, antitussive, antinociceptive, anti‐inflammatory, antibacterial, antiviral, and immunosuppressive activities. They also have antifungal, antioxidant, and anticancer activities. Phthalides are responsible for the characteristic odor of celery. Since ancient times, phthalides containing plants have been used in Asia, Europe, and North America as traditional medicines and food flavoring agents. In this review, we have provided the data regarding the mechanisms of action related with phthalides on different cancer cells, their chemopreventive properties, and their possible therapeutic potential against various types of human cancer. Our attempt is to make a case for the use of phthalide and their derivatives as one of the plant‐based compounds in targeting metabolic treatment of cancer diseases.

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Ligustilide inhibits the proliferation of non-small cell lung cancer via glycolytic metabolism

Cited by 18 publications

References 32 publications

Random Walk Algorithm-Based Computer Tomography (CT) Image Segmentation Analysis Effect of Spiriva Combined with Symbicort on Immunologic Function of Non-Small-Cell Lung Cancer

Random Walk Algorithm-Based Computer Tomography (CT) Image Segmentation Analysis Effect of Spiriva Combined with Symbicort on Immunologic Function of Non-Small-Cell Lung Cancer

Ligustilide counteracts carcinogenesis and hepatocellular carcinoma cell-evoked macrophage M2 polarization by regulating yes-associated protein-mediated interleukin-6 secretion

An Overview of Natural and Synthetic Phthalides Involved in Cancer Studies: Past, Present, and Future

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