Cervicovaginal microbiota dysbiosis correlates with HPV persistent infection

Bi, Qingqing; Zhu, Jun; Qu, Song-Ben; Chen, Juan; Zhang, Lei; Mu, Xiaojing

doi:10.1016/j.micpath.2020.104617

Cited by 34 publications

(34 citation statements)

References 25 publications

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“…The samples of CA and HSIL groups gradually deviating from the other groups. This finding was consistent with previous studies [16]. Therefore, we consider that with the progress of pathological status, the changes of microbiome are mainly reflected in the increase of bacterial diversity.…”

Section: Discussionsupporting

confidence: 94%

The feature of cervical microbiota associated with the progression of cervical cancer among reproductive females

Ding

Kong

et al. 2021

Gynecologic Oncology

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Cervical species diversity was higher in severer cervical intraepithelial neoplasia of reproductive-age women.• women with severer cervical pathology had more complex composition of cervical microbiota and more anaerobic bacteria.• Porphyromonas and Campylobacter were newfound marker genera of cervical cancer.• The correlation of differential functional pathways with Prevotella was opposite to that with Lactobacillus.• Marker genera in the lesion progression will be helpful for diagnosis, prevention and treatment.

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Section: Discussionsupporting

confidence: 94%

The feature of cervical microbiota associated with the progression of cervical cancer among reproductive females

Ding

Kong

et al. 2021

Gynecologic Oncology

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“…(678). Since there was no significant difference found in Good's coverage value among the samples (> 99%), reflects that sufficient amount of the bacterial diversity were captured in all the samples [45][46][47] . The Shannon index ranged from 5.177 to 7.613 and Simpson index ranged from 0.8985-0.9875 across the samples, indicates Polypheretima elongata had the lowest bacterial diversity, while Perionyx excavatus has the highest diversity, with high species richness.…”

Section: Discussionmentioning

confidence: 90%

Metagenomic Exploration of Bacterial Community Structure of Earthworms’ Gut

Thakur¹,

Lone²,

Tiwari³

et al. 2021

J. Pure Appl. Microbiol.

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Living organisms are naturally bestowed with unique and imitable qualities for maintaining ecological balance and earthworms are no exceptions. These so-called keystone species of terrestrial ecosystems are equipped with wonderful machinery, allowing them to nurture soil beautifully. Earthworm gut represents a potential microbial reservoir, having a complex interdependence with the host. The study aimed to profile bacterial community structure of three earthworm species belonging to two different life forms; Perionyx excavatus and Eudrilus eugeniae (epigeic), Polypheretima elongata (endogeic) respectively. Diversity analysis using 16S amplicon sequencing revealed that the dominant phyla were Proteobacteria (34.17-77.88) followed by Actinobacteria (13.43-35.54%), Firmicutes (1.69-15.45%) and Bacteroidetes (0.51-8.12%). The alpha diversity indices explicit similar gut microbiota of Perionyx excavatus and Eudrilus eugeniae and while higher alpha diversity was recorded in comparison to Polypheretima elongata gut. The taxonomic to the phenotypic annotation of 16S rRNA metagenomes revealed that dominance of Gram-negative bacterial community in all earthworm species while, Polypheretima elongata comprises higher percentage (78%) of Gram-negative bacterial community to Perionyx excavatus (32.3%) and Eudrilus eugeniae (38.3%). The oxygen requirement phenotypic analysis showed that all earthworm species were abundant with aerobic followed by anaerobic bacterial groups. Furthermore, functional metabolism phenotypic analysis revealed that a high abundance of ammonia oxidizers (29.3-80.2%), the gut microbiomes showed the relative abundance of sulphate reducer (22.6-78.7%), nitrite reducer (19.8-73.2%), dehalogenators (12.6-25.1%), illustrating in the role of these microbial communities in various degradation and bioremediation processes. The present study signifies the intrinsic gut microbiota of earthworm species for intensified biodegradation.

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“…Besides, many studies suggest that the cervicovaginal microbiota changes are closely related to HPV infection, persistence of infection, and HSIL [ 14 ], but the relationship with the HPV viral load was unclear. Menopausal women were more likely to develop HPV persistent infection; the possible reason was the decreased estrogen levels after menopause, leading to changes in cervicovaginal microbiota, which lost its protection against pathogens including HPV [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…is more likely to HPV infec-tion, and CST IV was related with an increased risk of transitioning to an HPV positive state [34,35]. Besides, some studies identified cervicovaginal microbiota dysbiosis closely related to HPV persistent infection, and transition between clusters was more frequent in women with persistent HPV16 infection (34%) than in women with transient infection (19%) [15,36] Based on the relationship with high HPV16/18 viral load in our study, CST IV has an enhanced effect on the risk of cervical high-grade lesions in women younger than 50 years old. However, for women over 50 years of age, vaginal microbiota dysbiosis is more common regardless normal or cervical lesions, as evidenced by the high proportion of CST IV in this study, so there is no significant association with HPV viral load among this population.…”

Section: Journal Of Immunology Researchmentioning

confidence: 99%

Age-Stratified Analysis of Vaginal Microbiota Dysbiosis and the Relationship with HPV Viral Load in HPV-Positive Women

Zhao

et al. 2022

Journal of Immunology Research

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Objective. This study evaluated the distribution of vaginal microbiota dysbiosis and the association with HPV viral load test in high-risk HPV-positive women before and after 50 years old. Methods. For this cross-sectional study, 388 HPV-positive women prior to referral to colposcopy in Peking University Peoples’ Hospital were included and classified as younger than 50 years ( n = 307 ) and aged 50 years or older ( n = 81 ), midvagina bacterial community composition was characterized by FlashDetect™ MAX vaginal microbe detection kit, and BMRT-HPV reported type-specific viral loads/10,000 cells. Results. The community state type (CST) IV was the most common CST occurring in 148 women (38.1%). The proportion of CST IV in those aged 50 years or older was significantly higher than those younger than 50 years (women) (66.7% vs. 30.6%); the difference was statistically significant (<0.001). CST distribution has no statistical difference in different grades of cervical lesion, regardless of the age ( p = 0.238 and 0.263). However, the women with high-grade cervical lesion presented a more complicated trend and the abundance of vaginal microbiota dysbiosis than low-grade lesion. HPV16/18 viral load was found to be significantly higher in CST III and CST IV than CST I/II/V ( p < 0.05 )in women younger than 50 years. Conclusions. In women younger than 50 years, higher HPV16/18 load was more closely associated with CST IV; however, it had no significant correlation in women aged 50 years or older.

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Cervicovaginal microbiota dysbiosis correlates with HPV persistent infection

Cited by 34 publications

References 25 publications

The feature of cervical microbiota associated with the progression of cervical cancer among reproductive females

The feature of cervical microbiota associated with the progression of cervical cancer among reproductive females

Metagenomic Exploration of Bacterial Community Structure of Earthworms’ Gut

Age-Stratified Analysis of Vaginal Microbiota Dysbiosis and the Relationship with HPV Viral Load in HPV-Positive Women

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