Arecoline enhances miR-21 to promote buccal mucosal fibroblasts activation

Yang, Hui; Yu, Cheng Chia; Hsieh, Pei‐Ling; Liao, Yi Wen; Chu, Pei Ming; Chen, Yu; Fang, Chih Yuan

doi:10.1016/j.jfma.2020.10.019

Cited by 28 publications

(8 citation statements)

References 31 publications

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“…After 48 h of incubation, the migrated cells on the lower surface of the membranes were stained with 0.1% crystal violet and quantified using ImageJ software (NIH, Bethesda, MD, United States). 19 …”

Section: Methodsmentioning

confidence: 99%

Targeting histone deacetylase 9 represses fibrogenic phenotypes in buccal mucosal fibroblasts with arecoline stimulation

Yang

Fang

Cho

et al. 2024

Journal of Dental Sciences

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Section: Methodsmentioning

confidence: 99%

Targeting histone deacetylase 9 represses fibrogenic phenotypes in buccal mucosal fibroblasts with arecoline stimulation

Yang

Fang

Cho

et al. 2024

Journal of Dental Sciences

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“…Moreover, miR-21 and myofibroblast marker, smooth muscle actin-alpha (α-SMA), were positively correlated. Therefore, the overexpression of miR-21 may be caused by the activation of areca nut components through the TGF-β pathway (Yang et al, 2021). Areca nut water extract induced p-SMAD2, an effector of TGF-β signaling, and TGF-β downstream targets, such as transglutaminase 2 (TGM2), transmembrane prostate androgen-induced RNA (TMEPAI), thrombospondin 1, and TGF-β1) in human epithelial keratinocyte HaCaT cells.…”

Section: Fibroblast Differentiationmentioning

confidence: 99%

Effects of areca nut consumption on cell differentiation of osteoblasts, myoblasts, and fibroblasts

Chang¹

2023

Biocell

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Areca nut is used worldwide as a hallucinogenic addicting drug along the tropical belt. Arecoline, a toxic compound, is the most important alkaloid in areca nuts. The adverse effects of oral uptake and chewing of areca nut are well known. For example, the possibility of cancer caused by chewing areca nuts is widely discussed. Chewing areca nut has other adverse effects on other organs, including abnormal cell differentiation, oral cancer, and several other diseases. The use of areca nut is also associated with low birthweight. Skeletal musculature is the largest organ in the body and is attached to the bones. During embryo development, the differentiation of bone and muscle cells is critical. In this article, we reviewed the effects of areca nut and arecoline on embryonic cell differentiation, particularly osteoblasts, myoblasts, and fibroblasts.

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“…During betel nut chewing, its physical and chemical stimuli directly impose on oral mucosa. Among these adverse stimuli, arecoline is a recognized chemical pathogenic factor for OSF, mainly activating fibroblasts to promote the collagen deposition and suppress the collagen degradation ( Yang et al, 2021 ). As fibrosis progresses, it can severely impair oral functions, such as eating, speaking, and swallowing.…”

Section: Introductionmentioning

confidence: 99%

Senescent epithelial cells remodel the microenvironment for the progression of oral submucous fibrosis through secreting TGF-β1

Wang

Han

Peng

et al. 2023

PeerJ

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Objectives Cellular senescence is strongly associated with fibrosis and tumorigenesis. However, whether the epithelium of oral submucous fibrosis (OSF) undergoes premature senescence remains unclear. This study investigates the roles of senescent epithelial cells in OSF. Methods The immunohistochemistry and Sudan black B staining were performed to identify epithelium senescence in OSF tissues. Arecoline was used to induce human oral keratinocytes (HOKs) senescence. The cell morphology, senescence-associated β galactosidase activity, cell counting Kit 8, immunofluorescence, quantitative real-time PCR, and western blot assay were used to identification of senescent HOKs. The enzyme-linked immunosorbent assay was exerted to evaluate the levels of transforming growth factor β1 (TGF-β1) in the supernatants of HOKs treated with or without arecoline. Results The senescence-associated markers, p16 and p21, were overexpressed in OSF epithelium. These expressions were correlated with alpha-smooth actin (α-SMA) positively and proliferating cell nuclear antigen (PCNA) negatively. Moreover, Sudan black staining showed that there was more lipofuscin in OSF epithelium. In vitro, HOKs treated with arecoline showed senescence-associated characteristics including enlarged and flattened morphology, senescence-associated β galactosidase staining, cell growth arrest, γH2A.X foci, upregulation of p53, p21, and TGF-β1 protein levels. Moreover, senescent HOKs secreted more TGF-β1. Conclusions Senescent epithelial cells are involved in OSF progression and may become a promising target for OSF treatment.

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Arecoline enhances miR-21 to promote buccal mucosal fibroblasts activation

Cited by 28 publications

References 31 publications

Targeting histone deacetylase 9 represses fibrogenic phenotypes in buccal mucosal fibroblasts with arecoline stimulation

Targeting histone deacetylase 9 represses fibrogenic phenotypes in buccal mucosal fibroblasts with arecoline stimulation

Effects of areca nut consumption on cell differentiation of osteoblasts, myoblasts, and fibroblasts

Senescent epithelial cells remodel the microenvironment for the progression of oral submucous fibrosis through secreting TGF-β1

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