Molecularly imprinted polymer nanogel-based fluorescence sensing of pork contamination in halal meat extracts

Cheubong, Chehasan; Takano, Eri; Kitayama, Yukiya; Sunayama, Hirobumi; Minamoto, Kazuaki; Takeuchi, Ryota; Furutani, Shunsuke; Takeuchi, Toshifumi

doi:10.1016/j.bios.2020.112775

Cited by 36 publications

(17 citation statements)

References 43 publications

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“…Another application of biomimetic sensors in detecting food adulteration was reported by Cheubong et al [ 84 ]. They developed a sensor based on fluorescence-labelled MIP nanogels, synthesized using their post imprinting method, to detect porcine serum albumin (PSA) as an indicator for pork contamination in halal meat extracts.…”

Section: Food Safetymentioning

confidence: 99%

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Biomimetic Sensors to Detect Bioanalytes in Real-Life Samples Using Molecularly Imprinted Polymers: A Review

Bräuer

Unger

Werner

et al. 2021

Sensors

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Molecularly imprinted polymers (MIPs) come with the promise to be highly versatile, useful artificial receptors for sensing a wide variety of analytes. Despite a very large body of literature on imprinting, the number of papers addressing real-life biological samples and analytes is somewhat limited. Furthermore, the topic of MIP-based sensor design is still, rather, in the research stage and lacks wide-spread commercialization. This review summarizes recent advances of MIP-based sensors targeting biological species. It covers systems that are potentially interesting in medical applications/diagnostics, in detecting illicit substances, environmental analysis, and in the quality control of food. The main emphasis is placed on work that demonstrates application in real-life matrices, including those that are diluted in a reasonable manner. Hence, it does not restrict itself to the transducer type, but focusses on both materials and analytical tasks.

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Section: Food Safetymentioning

confidence: 99%

“… Sensor signal for pork contamination in beef extract samples (0–100 wt%), n = 3 ( a ). The real-time PCR results of 1 wt% pork contamination in PBS and beef extract samples, n = 3 ( b ); Reproduced with permission from [ 84 ], © Elsevier. …”

Section: Figurementioning

confidence: 99%

Biomimetic Sensors to Detect Bioanalytes in Real-Life Samples Using Molecularly Imprinted Polymers: A Review

Bräuer

Unger

Werner

et al. 2021

Sensors

View full text Add to dashboard Cite

show abstract

“…In fact, we have developed a system for detecting contamination of halal food using fluorescent signaling MIP-nanogels. This system used the porcine serum albumin as the template and was found to detect pork contamination (0.1 wt.%) in beef extracts [38].…”

Section: Protein Sensing Mip Nanoparticles Prepared Via Pimsmentioning

confidence: 99%

Multi-Functional Nanocavities Fabricated Using Molecular Imprinting and Post-Imprinting Modifications for Efficient Biomarker Detection

Sunayama

Takeuchi

2021

Chromatography

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Antibodies and enzymes are currently considered the gold-standard molecular recognition elements as they facilitate the construction of biosensing systems and exhibit high specificity and affinity toward target molecules. However, the low stability of such systems and high associated production cost limit the practical applications of antibodies and enzymes, thereby necessitating the development of alternative molecular recognition elements. Molecularly imprinted polymers (MIPs) are synthetic polymer receptors that are capable of molecular recognition. These polymers contain binding cavities of various shapes and sizes that are complementary to the target molecule and aid in the capture of target molecules. However, although the original procedure for generating MIPs, developed before 2000, is simple, the resulting binding activity and selectivity are inferior to those of antibodies. Meanwhile, post-imprinting modification (PIM) involves site-directed chemical modification of functional monomer residues within the molecularly imprinted cavities to alter MIP functionality. In this review, we provide an overview of sophisticated PIM techniques for developing highly sensitive MIPs that can be used to recognize biomarker proteins. Toward this, we draw heavily on information from our own recent work. This article has the potential to provide important insights that would aid the development of synthetic polymer materials for biosensing.

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“…The concentration of HbA and HbA1c in blood are routinely measured for the determination of the degree of glycated HbA-the long-term diabetic parameter [6]. Recently MIP based sensors which apply the whole spectrum of electrochemical and optical transducers made inroads in the determination of protein biomarkers [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] including SARS-COV-2 antigens [8]. For many years, Bülow's group made great efforts to create HbA-specific polymer beads for chromatographic separation of HbA variants from protein mixtures and crude cell 2 of 13 extracts [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

“Out of Pocket” Protein Binding—A Dilemma of Epitope Imprinted Polymers Revealed for Human Hemoglobin

et al. 2021

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The epitope imprinting approach applies exposed peptides as templates to synthesize Molecularly Imprinted Polymers (MIPs) for the recognition of the parent protein. While generally the template protein binding to such MIPs is considered to occur via the epitope-shaped cavities, unspecific interactions of the analyte with non-imprinted polymer as well as the detection method used may add to the complexity and interpretation of the target rebinding. To get new insights on the effects governing the rebinding of analytes, we electrosynthesized two epitope-imprinted polymers using the N-terminal pentapeptide VHLTP-amide of human hemoglobin (HbA) as the template. MIPs were prepared either by single-step electrosynthesis of scopoletin/pentapeptide mixtures or electropolymerization was performed after chemisorption of the cysteine extended VHLTP peptide. Rebinding of the target peptide and the parent HbA protein to the MIP nanofilms was quantified by square wave voltammetry using a redox probe gating, surface enhanced infrared absorption spectroscopy, and atomic force microscopy. While binding of the pentapeptide shows large influence of the amino acid sequence, all three methods revealed strong non-specific binding of HbA to both polyscopoletin-based MIPs with even higher affinities than the target peptides.

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Molecularly imprinted polymer nanogel-based fluorescence sensing of pork contamination in halal meat extracts

Cited by 36 publications

References 43 publications

Biomimetic Sensors to Detect Bioanalytes in Real-Life Samples Using Molecularly Imprinted Polymers: A Review

Biomimetic Sensors to Detect Bioanalytes in Real-Life Samples Using Molecularly Imprinted Polymers: A Review

Multi-Functional Nanocavities Fabricated Using Molecular Imprinting and Post-Imprinting Modifications for Efficient Biomarker Detection

“Out of Pocket” Protein Binding—A Dilemma of Epitope Imprinted Polymers Revealed for Human Hemoglobin

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