Indole-based alkaloids from Ophiocordyceps xuefengensis

Qin, You; Zhou, Rongrong; Jin, Jian; Cheng, Fei; Shen, Bingbing; Zeng, Hongliang; Wan, Dan; Zhong, Can; Xie, Jing; Shen, Jun; Shi, Shuyun; Zhang, Shuihan

doi:10.1016/j.phytochem.2020.112536

Cited by 3 publications

(2 citation statements)

References 47 publications

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“…Similarly, indole-based alkaloids have been reported not only in Hypocreaceae but also in other Hypocreales species such as Ophiocordyceps xuefengensis and Clonostachys rosea ( Jiang et al . 2021 ; Qin et al . 2021 ).…”

Section: Resultsmentioning

confidence: 99%

Genomic insights into the evolution and adaptation of secondary metabolite gene clusters in fungicolous species Cladobotryum mycophilum ATHUM6906

Christinaki,

Myridakis,

Kouvelis

2024

G3: Genes, Genomes, Genetics

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Mycophilic or fungicolous fungi can be found wherever fungi exist since they are able to colonize other fungi, which occupy a diverse range of habitats. Some fungicolous species cause important diseases on basidiomycetes and thus, they are the main reason for the destruction of mushroom cultivations. Nonetheless, despite their ecological significance, their genomic data remain limited. Cladobotrum mycophilum is one of the most aggressive species of the genus, destroying the economically important Agaricus bisporus cultivations. The 40.7 Mb whole genome of the Greek isolate ATHUM6906 is assembled in 16 fragments, including the mitochondrial genome and two small circular mitochondrial plasmids, in this study. This genome includes a comprehensive set of 12,282 protein coding, 56 rRNA, and 273 tRNA genes. Transposable elements, CAZymes and pathogenicity related genes were also examined. The genome of C. mycophilum contained a diverse arsenal of genes involved in secondary metabolism, forming 106 Biosynthetic Gene Clusters, which renders this genome as one of the most BGC abundant among fungicolous species. Comparative analyses were performed for genomes of species of the family Hypocreaceae. Some BGCs identified in C. mycophilum genome exhibited similarities to clusters found in family Hypocreaceae, suggesting vertical heritage. In contrast, certain BGCs showed a scattered distribution among Hypocreaceae species or were solely found in Cladobotryum genomes. This work provides evidence of extensive BGC losses, Horizontal Gene Transfer events and formation of novel BGCs during evolution, potentially driven by neutral or even positive selection pressures. These events may increase Cladobotryum fitness under various environmental conditions and potentially during host-fungus interaction.

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Section: Resultsmentioning

confidence: 99%

Genomic insights into the evolution and adaptation of secondary metabolite gene clusters in fungicolous species Cladobotryum mycophilum ATHUM6906

Christinaki,

Myridakis,

Kouvelis

2024

G3: Genes, Genomes, Genetics

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“…Natural products contain hundreds of chemical compounds with different structures, contents, polarities, and pharmacological activities. Conventional bioactive compounds discovery programs are extraction, bioassay‐guided separation, concentration, and purification by repeated column chromatography, which are labor, resource, time, and cost intensive, and sometimes random and blind [2]. Thus, it has become desirable to develop effective methods for the rapid and efficient extraction and isolation of bioactive natural compounds.…”

Section: Introductionmentioning

confidence: 99%

Online extraction and enrichment coupling with high‐speed counter‐current chromatography for effective and target isolation of antitumor anthraquinones from seeds of Cassia obtusifolia

Fang

Cao

et al. 2021

J of Separation Science

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Traditional bioassay‐guided investigation of bioactive compounds from natural products comprises critical steps, such as extraction, repeated column separation, and activity assay. Thus, the development of facile, rapid, and efficient technology is critically important. Here, a HepG2 cell‐based extraction method was first developed to rapidly screen potential antitumor compounds from the seeds ofCassia obtusifolia. Then, an online extraction and enrichment–high‐speed counter‐current chromatography (HSCCC) strategy was fabricated to facilely and efficiently isolate target antitumor compounds, which included direct extraction from solid C. obtusifolia, removal of polar interferences, enrichment of target compounds, and preparative isolation by HSCCC using flow rate stepwise increasing mode. After further purification by Sephadex LH‐20 column, five antitumor anthraquinones, aurantio‐obtusin, 1‐desmethylaurantio‐obtusin, chryso‐obtusin, obtusin, and questin, were obtained for structural characterization and bioassay verification. The results may not only provide new perspectives for facile and rapid investigation of bioactive compounds from complex natural products, but also offer a scientific basis for the potential applications of C. obtusifolia.

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New insights into the chemistry and anticancer activity of Ophiocordyceps xuefengensis

Qin,

Zhou,

Liu

et al. 2024

Journal of Pharmaceutical and Biomedical Analysis

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Indole-based alkaloids from Ophiocordyceps xuefengensis

Cited by 3 publications

References 47 publications

Genomic insights into the evolution and adaptation of secondary metabolite gene clusters in fungicolous species Cladobotryum mycophilum ATHUM6906

Genomic insights into the evolution and adaptation of secondary metabolite gene clusters in fungicolous species Cladobotryum mycophilum ATHUM6906

Online extraction and enrichment coupling with high‐speed counter‐current chromatography for effective and target isolation of antitumor anthraquinones from seeds of Cassia obtusifolia

New insights into the chemistry and anticancer activity of Ophiocordyceps xuefengensis

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