Assessment of a natural grass carp reovirus genotype II avirulent strain GD1108 shows great potential as an avirulent live vaccine

Gao, Caixia; Wang, Yingying; Hu, Huzi; Zhou, Wenli; Yin, Jun; Li, Yingying; Bergmann, Sven; Wu, Siyu; Zeng, Weiwei; Wang, Qing

doi:10.1016/j.micpath.2020.104602

Cited by 5 publications

(6 citation statements)

References 56 publications

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“…Hemorrhagic disease of grass carp caused by GCRV remains the most serious infectious disease of grass carp and results in tremendous losses for the grass carp industry [ 14 , 26 ]. The three GCRV genotypes have proven to be highly genetically variable [ 10 , 18 , 24 , 27 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…GCRV is one of the most virulent agents of the genus Aquareovirus [ 44 ], and many GCRV have been reported to exhibit distinctive differences in virulence [ 10 , 18 , 24 , 26 , 33 , 44 ]. In general, genotype II is associated with high virulence and a short latent period, while genotype I is associated with low virulence and a longer latent period [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

“…GCRV possessed a double-layered capsid, and its genome is composed of 11 double-stranded RNA segments with a total size of approximately 24 kbp. Currently, there are more than 40 isolates of GCRV from diseased but also healthy grass carp around the world [ 9 , 10 , 18 , 24 , 25 , 26 ]. Based on differences in genome constitution and sequences, GCRV has been classified into three genotypes: GCRV-I represented by GCRV-873, GCRV-II represented by HZ08, and GCRV-III containing only a single member of Hubei grass carp disease reovirus (HGDRV), formerly isolate 104 [ 10 , 18 , 24 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

“…Both genotypes are low or not virulent for grass carp [ 9 , 10 , 30 , 31 , 32 , 33 ]. In contrast, GCRV-II does not produce CPE, but most are highly virulent to grass carp with mortality levels exceeding 80% [ 4 , 9 , 18 , 26 , 30 , 34 ]. However, the GCRV-II isolate GD1108 and CQ1807 are non-virulent, although the reason for this effect is currently unclear [ 26 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

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Identification, Virulence, and Molecular Characterization of a Recombinant Isolate of Grass Carp Reovirus Genotype I

Zeng

Bergmann

Dong

et al. 2021

Viruses

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The hemorrhagic disease of grass carp (HDGC) caused by grass carp reovirus (GCRV) still poses a great threat to the grass carp industry. Isolation and identification of the GCRV genotype I (GCRV-I) has been rarely reported in the past decade. In this study, a new GCRV was isolated from diseased fish with severe symptoms of enteritis and mild hemorrhages on the body surface. The isolate was further identified by cell culture, transmission electron, indirect immunofluorescence, and SDS-PAGE electrophoretic pattern analysis of genomic RNA. The results were consistent with the new isolate as a GCRV-I member and tentatively named GCRV-GZ1208. Both grass carp and rare minnow infected by the GCRV-GZ1208 have no obvious hemorrhagic symptoms, and the final mortality rate was ≤10%, indicating that it may be a low virulent isolate. GZ1208 possessed highest genomic homology to 873/GCHV (GCRV-I) and golden shiner reovirus (GSRV). Additionally, it was found a 90.7–98.3% nucleotide identity, a 96.4–100% amino acid identity, and <50% identity with GCRV-II and III genotypes. Interestingly, the sequences of some segments of GZ1208 were similar to GCRV-8733/GCHV, whereas the remaining segments were more closely related to GSRV, suggesting that a recombination event had occurred. Bootscan analysis of the complete genomic sequence confirmed this hypothesis, and recombination events between 873/GCHV and other GSRV-like viruses were also accompanied by gene mutations.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification, Virulence, and Molecular Characterization of a Recombinant Isolate of Grass Carp Reovirus Genotype I

Zeng

Bergmann

Dong

et al. 2021

Viruses

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“…Therefore, the development of new vaccines that can reduce or eliminate the disadvantages of inactivated vaccines and attenuated vaccines would be beneficial for grass carp aquaculture. Recently, an isolated avirulent strain GCRV‐GD1108 has been proven with good safety and immunogenicity and can be applied as a live vaccine 175 …”

Section: The Strategies To Suppress Aqrvs Infectionmentioning

confidence: 99%

Structure, function and immune evasion strategies of aquareoviruses, with focus on grass carp reovirus

Zhang

Chang

2023

Reviews in Aquaculture

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Aquareoviruses (AqRVs) can infect various wild or cultured bony fishes with serious muscle and viscera haemorrhage syndrome and have caused severe economic losses worldwide. Accumulating evidences have revealed the functions of viral proteins of AqRVs in RNA transcription, virion assembly, cell attachment and/or viral replication. In addition, structural information and transcription mechanism of AqRVs have been in‐depth studied through cryo‐electron microscopy combined with three‐dimensional image reconstruction. Although far from enough, structural and functional studies of AqRVs provide substantial insights into how viral proteins coordinate the replication of AqRVs and escape the host immune response. Grass carp reovirus (GCRV) is the most pathogenic AqRV isolated from aquatic animals. The completion of GCRV lifecycle requires the participation of host proteins. So far, many host factors involved in the entry and replication of GCRV have been identified. Based on the function of viral proteins and the viral lifecycle, many vaccines and antiviral agents have been developed to prevent the replication and infection of GCRV. This review summarises the structural characteristics and the composition of core proteins of AqRVs, as well as synergistic mechanisms of structural proteins on genome transcription and virus entry of AqRVs. In addition, the review highlights our current knowledge for the infection and replication, functions of non‐structural proteins, immune escape mechanisms, as well as the vaccines and antiviral agents of AqRVs, especially for GCRV. A better understanding of structure, function and immune evasion strategies of AqRVs will offer new targets for prevention and control of AqRVs in aquaculture.

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iTRAQ proteome analysis of insight into TLR20.2 functions through IFN1 signaling exerts regulatory effects on GCRV replication

Xiao

Huang

2023

Aquaculture

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Assessment of a natural grass carp reovirus genotype II avirulent strain GD1108 shows great potential as an avirulent live vaccine

Cited by 5 publications

References 56 publications

Identification, Virulence, and Molecular Characterization of a Recombinant Isolate of Grass Carp Reovirus Genotype I

Identification, Virulence, and Molecular Characterization of a Recombinant Isolate of Grass Carp Reovirus Genotype I

Structure, function and immune evasion strategies of aquareoviruses, with focus on grass carp reovirus

iTRAQ proteome analysis of insight into TLR20.2 functions through IFN1 signaling exerts regulatory effects on GCRV replication

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