Aptamer biorecognition-triggered hairpin switch and nicking enzyme assisted signal amplification for ultrasensitive colorimetric bioassay of kanamycin in milk

Liu, Mei; Yang, Zhenshan; Li, Baoxin; Du, Jianxiu

doi:10.1016/j.foodchem.2020.128059

Cited by 46 publications

(11 citation statements)

References 35 publications

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“…The biggest advantage of the colorimetric method is that preliminary experimental results can be obtained easily by direct observation of color changes using a cell phone chromatism or spectrophotometer without the need for complex equipment [82]. Consequently, the colorimetric method is inexpensive, simple, and rapid [83][84][85]. Gold nanoparticles (AuNPs) are an excellent material for colorimetric aptasensors because they have unique optical properties, good biocompatibility, large surface areas, and high absorption efficiencies.…”

Section: Colorimetric Aptamer Sensorsmentioning

confidence: 99%

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Luan

Wang

et al. 2020

Antibiotics

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Antibiotic abuse is becoming increasingly serious and the potential for harm to human health and the environment has aroused widespread social concern. Aminoglycoside antibiotics (AGs) are broad-spectrum antibiotics that have been widely used in clinical and animal medicine. Consequently, their residues are commonly found in animal-derived food items and the environment. A simple, rapid, and sensitive detection method for on-site screening and detection of AGs is urgently required. In recent years, with the development of molecular detection technology, nucleic acid aptamers have been successfully used as recognition molecules for the identification and detection of AGs in food and the environment. These aptamers have high affinities, selectivities, and specificities, are inexpensive, and can be produced with small batch-to-batch differences. This paper reviews the applications of aptamers for AG detection in colorimetric, fluorescent, chemiluminescent, surface plasmon resonance, and electrochemical sensors for the analysis in food and environmental samples. This study provides useful references for future research.

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Section: Colorimetric Aptamer Sensorsmentioning

confidence: 99%

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Luan

Wang

et al. 2020

Antibiotics

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Section: Introductionmentioning

confidence: 99%

“…Among the synthesis methods for colorimetric detection, the enzyme colorimetric sensing method has many advantages, such as high catalytic efficiency, good specificity and mild reaction conditions. 7 MOFs have attracted widespread attention due to their unique crystal structure and have been widely used in storage, separation, catalysis, sensing, and other aspects. [8][9][10] In recent years, some MOFs, such as Cu-or Fe-based MOFs, have shown good peroxidase-like activity.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive colorimetric detection and effective adsorption of phosphate based on MOF-808(Zr/Ce)

Zhai

Hou

et al. 2022

New J. Chem.

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“…Recently, an ultrasensitive colorimetric sensor was developed for detecting kanamycin based on an aptamer biorecognition-triggered hairpin switch and nicking-enzyme-assisted signal amplification, which obtained a LOD of 0.2 pg/mL [ 107 ]. As shown in Figure 4 E, the hairpin probe contained three parts: kanamycin aptamer, target DNA (T-DNA) located in the hairpin ring, and extended DNA (E-DNA) as an auxiliary stabilizer to the hairpin structure.…”

Section: Nanozyme-based Aptasensorsmentioning

confidence: 99%

“…( D ) A nanozyme-based cascade colorimetric aptasensor for OTA detection [ 106 ]. ( E ) A colorimetric bioassay for detecting kanamycin based on aptamer biorecognition-triggered hairpin switch and nicking-enzyme-assisted signal amplification [ 107 ]. ( F ) Ratiometric dual signal-enhancing-based electrochemical biosensor for ultrasensitive kanamycin detection [ 108 ].…”

Section: Figurementioning

confidence: 99%

Regulation Mechanism of ssDNA Aptamer in Nanozymes and Application of Nanozyme-Based Aptasensors in Food Safety

Wang

Zhou

et al. 2022

Foods

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Food safety issues are a worldwide concern. Pathogens, toxins, pesticides, veterinary drugs, heavy metals, and illegal additives are frequently reported to contaminate food and pose a serious threat to human health. Conventional detection methods have difficulties fulfilling the requirements for food development in a modern society. Therefore, novel rapid detection methods are urgently needed for on-site and rapid screening of massive food samples. Due to the extraordinary properties of nanozymes and aptamers, biosensors composed of both of them provide considerable advantages in analytical performances, including sensitivity, specificity, repeatability, and accuracy. They are considered a promising complementary detection method on top of conventional ones for the rapid and accurate detection of food contaminants. In recent years, we have witnessed a flourishing of analytical strategies based on aptamers and nanozymes for the detection of food contaminants, especially novel detection models based on the regulation by single-stranded DNA (ssDNA) of nanozyme activity. However, the applications of nanozyme-based aptasensors in food safety are seldom reviewed. Thus, this paper aims to provide a comprehensive review on nanozyme-based aptasensors in food safety, which are arranged according to the different interaction modes of ssDNA and nanozymes: aptasensors based on nanozyme activity either inhibited or enhanced by ssDNA, nanozymes as signal tags, and other methods. Before introducing the nanozyme-based aptasensors, the regulation by ssDNA of nanozyme activity via diverse factors is discussed systematically for precisely tailoring nanozyme activity in biosensors. Furthermore, current challenges are emphasized, and future perspectives are discussed.

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Aptamer biorecognition-triggered hairpin switch and nicking enzyme assisted signal amplification for ultrasensitive colorimetric bioassay of kanamycin in milk

Cited by 46 publications

References 35 publications

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Highly sensitive colorimetric detection and effective adsorption of phosphate based on MOF-808(Zr/Ce)

Regulation Mechanism of ssDNA Aptamer in Nanozymes and Application of Nanozyme-Based Aptasensors in Food Safety

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