Application of pulsed electric fields for the biocompatible extraction of proteins from the microalga Haematococcus pluvialis

Gâteau, Hélène; Blanckaert, Vincent; Veidl, Brigitte; Burlet‐Schiltz, Odile; Pichereaux, Carole; Gargaros, Audrey; Marchand, Justine; Schoefs, Benoı̂t

doi:10.1016/j.bioelechem.2020.107588

Cited by 29 publications

(19 citation statements)

References 78 publications

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“…Small cells (around µm 3 ) with a circular membrane (around 0.9 in circularity) were able to maintain t the solvent, many cells loaded EB and appeared dark in colour, meaning that naturally permeabilized (NAT-P) subpopulation accounted for 36% of the microalgae tested (Figure 6e). This proportion is similar to that reported in the literature (Gateau et al, 2021) [8]. Among the NAT-P subpopulation, some cells presented an irregular membrane and were partially opened (Figure 6a).…”

Section: Cell Permeabilization With Solventsupporting

confidence: 90%

“…In agreement with Gateau et al [8] cells can range into 5 categories, i.e., (1) the irreversibly permeabilized (IP), (2) the reversibly permeabilized (RP), (3) the naturally permeabilized (NAT-P), (4) the non-affected cells (NA) and ( 5) disrupted cells (DC), (Table 1). Thus, after mixing with solvent, a sample contained a certain proportion of each cell category.…”

Section: Cell Membrane Permeabilizationsupporting

confidence: 83%

“…For longer mixing time, the RP cells became progressively less abundant while that of IP remained constant (Figure 6e). Gateau et al [8] have coined the biocompatibility index (BI) to describe the evolution of such populations during a permeabilizing treatment [8]. Figure 6b displays the evolution of BI along the n-decane treatment.…”

Section: Cells (Ip) and Reversibly Permeabilized Cells (Rp) For The Different Extraction Time (B) Biocompatibility Index Throughout The Tmentioning

confidence: 99%

“…To reduce production residues, in situ extraction methods or milking have been proposed. This can include the use of pre-treatments such as electric pulses [7,8], resonance frequency [9], or biocompatible solvents [10][11][12][13][14]. In Dunaliella salina, by comparing β-carotene solubility, solvent extraction ability and biocompatibility of organic solvents, León et al (2003) established that the best compromised for β-carotene biocompatible extraction was achieved with hydrophobic solvents exhibiting a log P octanol around five such as n-decane, provided that the solvent/culture ratio (v/v) remains less than 1 [12,13].…”

Section: Introductionmentioning

confidence: 99%

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Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from Dunaliella salina

et al. 2021

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Biocompatible extraction emerges recently as a means to reduce costs of biotechnology processing of microalgae. In this frame, this study aimed at determining how specific culture conditions and the associated cell morphology impact the biocompatibility and the extraction yield of β-carotene from the green microalga Dunaliella salina using n-decane. The results highlight the relationship between the cell disruption yield and cell volume, the circularity and the relative abundance of naturally permeabilized cells. The disruption rate increased with both the cell volume and circularity. This was particularly obvious for volume and circularity exceeding 1500 µm3 and 0.7, respectively. The extraction of β-carotene was the most biocompatible with small (600 µm3) and circular cells (0.7) stressed in photobioreactor (30% of carotenoids recovery with 15% cell disruption). The naturally permeabilized cells were disrupted first; the remaining cells seems to follow a gradual permeabilization process: reversibility (up to 20 s) then irreversibility and cell disruption. This opens new carotenoid production schemes based on growing robust β-carotene enriched cells to ensure biocompatible extraction.

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Section: Cell Permeabilization With Solventsupporting

confidence: 90%

Section: Cell Membrane Permeabilizationsupporting

confidence: 83%

Section: Cells (Ip) and Reversibly Permeabilized Cells (Rp) For The Different Extraction Time (B) Biocompatibility Index Throughout The Tmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from Dunaliella salina

et al. 2021

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“…The PEF treatment resulted in enhanced extraction of the functional components with high purity, which can be used for their nutraceutical application in food industries without purification. A similar study for extraction from the microalgae Haematococcus pluvialis is reported by Gateau et al ( 146 ). Other studies were on extraction from apple peels ( 147 ), cyanobacteria ( 148 ), tomato ( 149 ), and from cinnamon ( 150 ).…”

Section: Non-thermal Technologiessupporting

confidence: 64%

Non-thermal Technologies for Food Processing

2021

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Food is subjected to various thermal treatments during processes to enhance its shelf-life. But these thermal treatments may result in deterioration of the nutritional and sensory qualities of food. With the change in the lifestyle of people around the globe, their food needs have changed as well. Today's consumer demand is for clean and safe food without compromising the nutritional and sensory qualities of food. This directed the attention of food professionals toward the development of non-thermal technologies that are green, safe, and environment-friendly. In non-thermal processing, food is processed at near room temperature, so there is no damage to food because heat-sensitive nutritious materials are intact in the food, contrary to thermal processing of food. These non-thermal technologies can be utilized for treating all kinds of food like fruits, vegetables, pulses, spices, meat, fish, etc. Non-thermal technologies have emerged largely in the last few decades in food sector.

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