Metabolite profiling and evaluation of CYP450 interaction potential of ‘Trimada’- an Ayurvedic formulation

Kar, Amit; Saha, Sankarshan; Banerjee, Subhadip; Goswami, Debkalpa; Matsabisa, Motlalepula G.; Charoensub, Rawiwan; Duangyod, Thidarat

doi:10.1016/j.jep.2020.113457

Cited by 11 publications

(6 citation statements)

References 19 publications

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“…Our results also showed that the potential inhibitors of CYP3A4 had the similar effect of miR-4277 on sorafenib in HCC cells. The effect of ketoconazole is significantly stronger than amprenavir or diltiazem (49)(50)(51)(52)(53)(54)(55). Since ketoconazole is also considered to be an inhibitor of PXR, this needs to be discussed in depth.…”

Section: Discussionmentioning

confidence: 99%

Hsa-miR-4277 Decelerates the Metabolism or Clearance of Sorafenib in HCC Cells and Enhances the Sensitivity of HCC Cells to Sorafenib by Targeting cyp3a4

Sun

Jiang

et al. 2021

Front. Oncol.

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Increasing evidence has shown that the metabolism and clearance of molecular targeted agents, such as sorafenib, plays an important role in mediating the resistance of HCC cells to these agents. Metabolism of sorafenib is performed by oxidative metabolism, which is initially mediated by CYP3A4. Thus, targeting CYP3A4 is a promising approach to enhance the sensitivity of HCC cells to chemotherapeutic agents. In the present work, we examined the association between CYP3A4 and the prognosis of HCC patients receiving sorafenib. Using the online tool miRDB, we predicted that has-microRNA-4277 (miR-4277), an online miRNA targets the 3’UTR of the transcript of cyp3a4. Furthermore, overexpression of miR-4277 in HCC cells repressed the expression of CYP3A4 and reduced the elimination of sorafenib in HCC cells. Moreover, miR-4277 enhanced the sensitivity of HCC cells to sorafenib in vitro and in vivo. Therefore, our results not only expand our understanding of CYP3A4 regulation in HCC, but also provide evidence for the use of miR-4277 as a potential therapeutic in advanced HCC.

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Section: Discussionmentioning

confidence: 99%

Hsa-miR-4277 Decelerates the Metabolism or Clearance of Sorafenib in HCC Cells and Enhances the Sensitivity of HCC Cells to Sorafenib by Targeting cyp3a4

Sun

Jiang

et al. 2021

Front. Oncol.

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“…The toxicity of PHELA ® extract in HEPG2 cells were performed using MTT cytotoxicity assay with some modification as described by Kar et al [19]. HEPG2 cells were cultured and maintained in high glucose Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% (v/v) fetal bovine serum, L-glutamine (4 mM), 100 U/mL penicillin and 100 µg/mL streptomycin.…”

Section: Safety Assessment Of Phela ® Extract 261 Mtt Cytotoxicity Assaymentioning

confidence: 99%

“…Product formation from the fluorogenic substrate were measured on a fluorescence microplate reader (PHERAstar, BMG Labtech, Cary, NC, USA) using an excitation wavelength of 415 nm and emission wavelength of 460 nm. Percentage inhibition and IC 50 value were determined [19,20]. Broth dilution is one of the most basic antimicrobial susceptibility testing methods to determine MIC using a 96-well microtitre plate [21].…”

Section: Cyp450 Enzyme Inhibition Studymentioning

confidence: 99%

“…The study was performed as described by Sarkar et al [19]. In a 96=well microtitre plate, 25 µL of a 1.5 × 10 8 CFU/mL suspension of S. aureus was inoculated in 100 µL of fresh MHB in each well followed by the addition of 50 µL of the extract (MIC, MIC X 2, MIC X 3 and MIC X 4) or standard antimicrobial agent (ciprofloxacin) at a sub-lethal concentration and serially diluted row-wise.…”

Section: Inhibition Of Biofilm Formationmentioning

confidence: 99%

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Quality Related Safety Evaluation of a South African Traditional Formulation (PHELA®) as Novel Anti-Biofilm Candidate

et al. 2022

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“…nigrum L. is a common herbal ingredient in the remedies that are listed in the national list of essential medicines of the Thailand Food and Drug Administration, such as the Prab-Chom-Phu-Ta-Weep and Ar-Phai-Sa-Lee remedies, which is used to relieve first-stage cold symptoms and allergic rhinitis. However, to meet the increasing demand in the market for herbal raw materials, P. nigrum L. samples are vulnerable to adulteration and contamination [23]. The development of quality standardization parameters of Thai P. nigrum L. is crucial.…”

Section: Introductionmentioning

confidence: 99%

Metabolite Fingerprinting of Piper nigrum L. from Different Regions of Thailand by UHPLC-QTOF-MS Approach and In Vitro Bioactivities

Jaidee

Maneerat²,

Rujanapun³

et al. 2022

Trends Sci

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This study aimed to profile the metabolites, piperine content, and biological activities (antioxidant, anti-inflammatory, anticancer, antibacterial, and apoptotic cell) of 15 samples of Piper nigrum L. from various geographic regions of Thailand. UHPLC-QTOF-MS was applied to establish alkaloid profiles of the P. nigrum L. samples. PCA and HCA analysis showed that the samples from the PN-1, PN-2, and PN-11 sources differed significantly from those of other sources in terms of compound type and alkaloid concentration profile. Comparative analysis based on a validated HPLC-DAD method was used for the standardization of 15 varieties of P. nigrum L. with piperine as a standard phytomarker which revealed that PN-4 source possessed a high content of piperine. PN-2 showed the highest antioxidant capacity (DPPH activity) while PN-10, PN-12, and PN-15 showed the highest FRAP activity. PN-11 source showed high inhibition of anti-inflammatory and anticancer properties. Furthermore, PN-3, and PN-4 also showed the highest both anti-inflammatory and antibacterial activity. Almost samples have been shown to induce late apoptosis and necrosis in SW480 cell line. This study will also be useful for the development of quality control parameters through the standardization of varieties of P. nigrum L. as raw materials in traditional medicine formulations. HIGHLIGHTS Metabolite profiling of the nigrum L. varieties from 15 different geographical locations in Thailand Quality control parameter for standardization based on marker analysis by HPLC-DAD using piperine as the marker compound Comparisons of metabolites among 15 different cultivations by Principal Component Analysis (PCA) and hierarchical cluster analysis (HCA) Biological activity of the nigrum L. varieties from 15 different geographical locations in Thailand GRAPHICAL ABSTRACT

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Metabolite profiling and evaluation of CYP450 interaction potential of ‘Trimada’- an Ayurvedic formulation

Cited by 11 publications

References 19 publications

Hsa-miR-4277 Decelerates the Metabolism or Clearance of Sorafenib in HCC Cells and Enhances the Sensitivity of HCC Cells to Sorafenib by Targeting cyp3a4

Hsa-miR-4277 Decelerates the Metabolism or Clearance of Sorafenib in HCC Cells and Enhances the Sensitivity of HCC Cells to Sorafenib by Targeting cyp3a4

Quality Related Safety Evaluation of a South African Traditional Formulation (PHELA®) as Novel Anti-Biofilm Candidate

Metabolite Fingerprinting of Piper nigrum L. from Different Regions of Thailand by UHPLC-QTOF-MS Approach and In Vitro Bioactivities

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