The key role of delignification in overcoming the inherent recalcitrance of Chinese fir for biorefining

Ouyang, Shuiping; Shi, Jinjie; Qiao, Hui; Zheng, Zhaojuan; Ouyang, Jia; Lai, Chenhuan

doi:10.1016/j.biortech.2020.124154

Cited by 17 publications

(1 citation statement)

References 35 publications

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“…Additionally, at the 3 h mark of SC pretreatment, cellulose and hemicellulose loss remained below five percent, signifying minimal carbohydrate loss during this processing step. Generally, sodium chlorite’s optimal temperature range for pretreatment falls between 60 and 80 °C; however, changes in temperature exert limited effects on reaction rates due to sodium chlorite’s propensity to decompose at higher temperatures [ 22 , 37 , 38 , 39 ]. In summary, the pretreatment method involving 10% ( w / v ) solid loading, 5% ( w / v ) SC, and a retention time of 3 h at 70 °C was selected.…”

Section: Resultsmentioning

confidence: 99%

Highly Efficient Production of Cellulosic Ethanol from Poplar Using an Optimal C6/C5 Co-Fermentation Strain of Saccharomyces cerevisiae

Xu,

Sun,

Wang

et al. 2024

Microorganisms

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Cellulosic ethanol is the key technology to alleviate the pressure of energy supply and climate change. However, the ethanol production process, which is close to industrial production and has a high saccharification rate and ethanol yield, still needs to be developed. This study demonstrates the effective conversion of poplar wood waste into fuel-grade ethanol. By employing a two-step pretreatment using sodium chlorite (SC)-dilute sulfuric acid (DSA), the raw material achieved a sugar conversion rate exceeding 85% of the theoretical value. Under optimized conditions, brewing yeast co-utilizing C6/C5 enabled a yield of 35 g/L ethanol from 10% solid loading delignified poplar hydrolysate. We increased the solid loading to enhance the final ethanol concentration and optimized both the hydrolysis and fermentation stages. With 20% solid loading delignified poplar hydrolysate, the final ethanol concentration reached 60 g/L, a 71.4% increase from the 10% solid loading. Our work incorporates the pretreatment, enzymatic hydrolysis, and fermentation stages to establish a simple, crude poplar waste fuel ethanol process, expanding the range of feedstocks for second-generation fuel ethanol production.

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