[33] A convenient biosynthetic method for the preparation of radioactive flavin nucleotides using Clostridium kluyveri

CoASH and some of its acyl derivatives, especially acetyl-SCoA, occupy a central position in the energy metabolism of the anaerobic Clostridium kluyveri, both as intermediates and as regulatory effectors. The steady state concentrations of these compounds were determined in growing cultures of this organism using an anaerobic and fast deproteinization technique and radio isotope assays. Acetyl-SCoA was determined as [1-14C]citrate formed in the presence of [4-14C]oxaloacetate and citrate synthase; 0.49 mumol/g cell wet wt. were found. CoASH, CoAS-SCoA after borohydride reduction, and total acyl derivatives of coenzyme A after hydrolysis of the thiol esters were converted to thioethers with [2,3-14C]N-ethylmaleimide and brought to radiochemical purity by chromatographic methods. While disulfides of coenzyme A were undetectable, 0.13 mumol CoASH and 1.17 mumol of total acyl-SCoA per g wet wt. were found. These data are consistent with the regulatory scheme of the energy metabolism of C. kluyveri previously proposed.

show abstract

[33] A convenient biosynthetic method for the preparation of radioactive flavin nucleotides using Clostridium kluyveri

Cited by 7 publications

References 10 publications

One of the Fumarate Reductase Isoenzymes fromSaccharomyces cerevisiaeIs Encoded by theOSM1Gene

One of the Fumarate Reductase Isoenzymes fromSaccharomyces cerevisiaeIs Encoded by theOSM1Gene

Bioconversions

Acetyl coenzyme A and coenzyme A contents of growing Clostridium kluyveri as determined by isotope assays

Contact Info

Product

Resources

About