Bmintegrin β1: A broadly expressed molecule modulates the innate immune response of Bombyx mori

Li, Chongyang; Zhang, Kui; Pan, Guoqing; Hu, Xin; Zhao, Gaichao; Deng, Chaowei; Tan, Mengqin; Li, Changhong; Xu, Man; Su, Jingjing; Шен, Ли; Kausar, Saima; Yang, Liqun; Abbas, Muhammad Nadeem; Cui, Hongjuan

doi:10.1016/j.dci.2020.103869

Cited by 17 publications

(10 citation statements)

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“…In B. mori , β3 integrin has been shown to be extensively produced in response to bacterial and pathogen-associated molecular patterns (PAMPs), implying that these proteins are likely involved in hemocyte-mediated immunity ( 37 ). Another study recently observed comparable expression patterns of integrin proteins in hemocytes, confirming the hemocyte-associated biological roles of integrins ( 35 ).…”

Section: The Biological Role Of Integrins In Immunological Responses ...supporting

confidence: 59%

“…Therefore, it was considered that integrins are only associated with hemocytes or blood cells in animals ( 29 , 37 ). In contrast, a recent study on B. mori suggested that integrins are found in hemocytes and also expressed in other tissues, including fat bodies ( 35 ). The fat body is a multifunctional tissue that involves integrating signals, synthesizing immune system proteins, serving as a center of metabolism, regulating molting and metamorphosis, and synthesizing hormones that regulate body functions.…”

Section: The Biological Role Of Integrins In Immunological Responses ...mentioning

confidence: 94%

“…For example, In P. includens and M. sexta , α1 and α3 are mainly produced in hemocytes, α2 is widely transcribed in fat bodies but lowly expressed in hemocytes, and β1 is specifically expressed only in hemocytes ( 32 – 34 ). β1, on the other hand, is strongly expressed in fat bodies ( 35 ). While β1 is predominantly expressed in hemocytes in O. furnacalis ( 36 ).…”

Section: Integrins Are Components Of Immune Cellsmentioning

confidence: 99%

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Integrins in the Immunity of Insects: A Review

et al. 2022

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Integrins are a large group of cell-surface proteins that are classified as transmembrane proteins. Integrins are classified into different types based on sequence variations, leading to structural and functional diversity. They are broadly distributed in animals and have a wide range of biological functions such as cell-to-cell communication, intracellular cytoskeleton organization, cellular signaling, immune responses, etc. Integrins are among the most abundant cell surface proteins in insects, exhibiting their indispensability in insect physiology. Because of their critical biological involvement in physiological processes, they appear to be a novel target for designing effective pest control strategies. In the current literature review, we first discuss the discovery and expression responses of integrins against various types of pathogens. Secondly, we examine the specific biological roles of integrins in controlling microbial pathogens, such as phagocytosis, encapsulation, nodulation, immune signaling, and so on. Finally, we describe the possible uses of integrins to control agricultural insect pests.

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Section: The Biological Role Of Integrins In Immunological Responses ...supporting

confidence: 59%

Section: The Biological Role Of Integrins In Immunological Responses ...mentioning

confidence: 94%

Section: Integrins Are Components Of Immune Cellsmentioning

confidence: 99%

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Integrins in the Immunity of Insects: A Review

et al. 2022

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“…They went on to say that some bacteria can induce both the Toll and IMD pathways, suggesting that there is some crosstalk between these immune-signaling pathways. Additionally, integrin, a cell surface receptor protein that has been reported to contribute to encapsulation, pathogen adherence, and phagocytosis, was increased in addition to these immune-pathway-related genes [ 56 , 57 ]. Here, we discussed our results very precisely because our data showed that the inhibition of DNA methylation has an impact on a variety of physiological processes, such as development.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Sequencing Highlights the Regulatory Role of DNA Methylation in Immune-Related Genes’ Expression of Chinese Oak Silkworm, Antheraea pernyi

Kausar

Liu

Gul

et al. 2022

Insects

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Antheraea pernyi is an important lepidopteran used as a model insect species to investigate immune responses, development, and metabolism modulation. DNA methylation has recently been found to control various physiological processes throughout the life of animals; however, DNA methylation and its effect on the physiology of insects have been poorly investigated so far. In the present study, to better understand DNA methylation and its biological role in the immune system, we analyzed transcriptome profiles of A. pernyi pupae following DNA methylation inhibitor injection and Gram-positive bacteria stimulation. We then compared the profiles with a control group. We identified a total of 55,131 unigenes from the RNA sequence data. A comparison of unigene expression profiles showed that a total of 680 were up-regulated and 631 unigenes were down-regulated in the DNA-methylation-inhibition-bacteria-infected group compared to the control group (only bacteria-injected pupae), respectively. Here, we focused on the immune-related differentially expressed genes (DEGs) and screened 10 genes that contribute to immune responses with an up-regulation trend, suggesting that microbial pathogens evade host immunity by increasing DNA methylation of the host genome. Furthermore, several other unigenes related to other pathways were also changed, as shown in the KEGG analysis. Taken together, our data revealed that DNA methylation seems to play a crucial biological role in the regulation of gene expression in insects, and that infection may enhance the host genome DNA methylation by a yet-unknown mechanism.

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“…The larvae were reared on clean, fresh mulberry leaves at 25 °C under 60%–90% relative humidity and a 16 : 8 h light : dark photoperiod. Embryonic and larval tissue samples were collected under sterile conditions (Li et al ., 2020). All samples were immediately frozen using liquid nitrogen and stored at −80 °C.…”

Section: Methodsmentioning

confidence: 99%

Bombyx mori U‐shaped regulates the melanization cascade and immune response via binding with the Lozenge protein

et al. 2021

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Zinc finger protein, an important transcription factor, regulates gene expression associated with various physiological and pathological processes. U‐shaped, belong to the Friend of GATA (FOG) transcription factor, plays a crucial role in hematopoiesis by interacting with the GATA transcription factor as a co‐factor. However, little is known about its functions in insects. In the present study, a U‐shaped cDNA was identified and characterized from the silkworm Bombyx mori and its potential roles in innate immunity investigated. The predicted silkworm U‐shaped amino acid sequence contained a classical nuclear localization signal (NLS) motif “GESSPKRRRR” at position 450–459, and arginine residues at position 456 and 478 are the critical sites of the NLS. U‐shaped mRNA was detected in all tested tissues of the B. mori; however, the highest levels were found in the hemocytes. U‐shaped mRNA expression levels were upregulated in the hemocyte after the Escherichia coli and Staphylococcus aureus challenge. Furthermore, U‐shaped knockdown significantly reduced the melanization process and suppressed the expression of melanization‐associated genes, including PPO1, PPO2, PPAE and BAEE. In addition, U‐shaped interacts with Lozenge protein to regulate the innate immune response of the insect. Our results revealed that U‐shaped binds directly to Lozenge protein to modulate the melanization process and innate immune responses in silkworm.

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Bmintegrin β1: A broadly expressed molecule modulates the innate immune response of Bombyx mori

Cited by 17 publications

References 54 publications

Integrins in the Immunity of Insects: A Review

Integrins in the Immunity of Insects: A Review

Transcriptome Sequencing Highlights the Regulatory Role of DNA Methylation in Immune-Related Genes’ Expression of Chinese Oak Silkworm, Antheraea pernyi

Bombyx mori U‐shaped regulates the melanization cascade and immune response via binding with the Lozenge protein

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