A scalable and reproducible manufacturing process for Phlebotomus papatasi salivary protein PpSP15, a vaccine candidate for leishmaniasis

Liu, Zhuyun; Kundu, Rakhi; Damena, Surafel; Biter, Amadeo B.; Nyon, Mun Peak; Chen, Wen‐Hsiang; Zhan, Bin; Strych, Ulrich; Hotez, Peter J.; Bottazzi, María Elena

doi:10.1016/j.pep.2020.105750

Cited by 4 publications

(2 citation statements)

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“…According to WHO's report in 2017, more than 95% of new cases of CL occurred in Afghanistan, Algeria, Brazil, Colombia, Iran, Iraq, and Syria. [ 6 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Application of BASNEF model in students training regarding cutaneous leishmaniasis prevention behaviors: a school-based quasi experimental study

2021

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Background Cutaneous leishmaniasis (CL) is endemic in 98 countries, and 350 million people are at risk of the disease worldwide. In endemic areas, conducting educational interventions is necessary to change preventive behaviors of CL. This study aimed to investigate the effect of an educational intervention based on the BASNEF model on CL preventive behavior in students. Methods The present quasi-experimental study examined 80 students living in endemic areas of leishmaniasis in Isfahan province, Iran based on the BASNEF model. The required data were collected twice before and two months after the educational intervention based on a questionnaire whose validity and reliability had been already proven in other studies. The intervention was performed in three educational sessions for the students in the intervention group and 1 educational session for teachers and parents. Data were analyzed by SPSS (VER26) using the chi-square test, independent t-test, analysis of covariance (ANCOVA), and Paired t-test. Results After intervention, the mean scores of Knowledge (P < 0.001), attitude (P = 0.02), subjective norms (P = 0.04), behavioral intention (P < 0.001), and behavior (P = 0.02) indicated significant differences between the intervention and control groups, but an increase in mean scores of enabling factors was not significant (P = 0. 93). Conclusions Providing students with the educational intervention based on the BASNEF model improve their ability to the extent that they transmit these educations to their family members, which would be effective in preventing and controlling CL in leishmaniasis-prone areas. Trial registration Name: Iranian Registry of Clinical Trials. Registration number: IRCT20201024049131N1. Registration date: 2020–11-20. Registration timing: prospective.

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“…According to WHO's report in 2017, more than 95% of new cases of CL occurred in Afghanistan, Algeria, Brazil, Colombia, Iran, Iraq, and Syria. [ 6 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Application of BASNEF model in students training regarding cutaneous leishmaniasis prevention behaviors: a school-based quasi experimental study

2021

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“…The research results of Zhu and colleagues showed that the expression product of P. pastoris had better rapid performance and immune effects than the expression product of E. coli [ 38 ]. To date, P. pastoris has been successfully used to produce AMPs (such as TH2-3), enzyme preparations (such as xylanase) and vaccines (such as PpSP15) [ 39 – 41 ]. Therefore, this study chose P. pastoris as the production factory for LG.…”

Section: Resultsmentioning

confidence: 99%

Design and heterologous expression of a novel dimeric LL37 variant in Pichia pastoris

et al. 2021

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Background The antimicrobial peptide LL37 is produced by white blood cells (mainly neutrophils) and various epithelial cells, and has the outstanding advantages of participating in immune regulation, causing chemotaxis of immune cells and promoting wound healing. However, the central domain of LL37 needs to be improved in terms of antimicrobial activity. Results In this study, the amino acid substitution method was used to improve the antimicrobial activity of the LL37 active center, and a dimeric design with a better selection index was selected. A flexible linker was selected and combined with the 6 × His-SUMO tag and LG was successfully expressed using Pichia pastoris as a host. Recombinant LG displayed strong antimicrobial activity by destroying the cell membrane of bacteria but had low hemolytic activity. In addition, compared with monomeric peptide FR, rLG had improved ability to tolerate salt ions. Conclusion This research provides new ideas for the production of modified AMPs in microbial systems and their application in industrial production.

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Anti-Parasite Agents and Vaccines

Ridi¹

2022

Encyclopedia of Infection and Immunity

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A scalable and reproducible manufacturing process for Phlebotomus papatasi salivary protein PpSP15, a vaccine candidate for leishmaniasis

Cited by 4 publications

References 32 publications

Application of BASNEF model in students training regarding cutaneous leishmaniasis prevention behaviors: a school-based quasi experimental study

Application of BASNEF model in students training regarding cutaneous leishmaniasis prevention behaviors: a school-based quasi experimental study

Design and heterologous expression of a novel dimeric LL37 variant in Pichia pastoris

Anti-Parasite Agents and Vaccines

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