32. Sub-Kelvin resistance, impact resistance, and neutron dose of the tardigrade: Ramazzottius varieoranatus

Kletetschka, Günther; Horikawa, Daiki D.; Mikula, V. A.; Parsons, A. M.; Bodnarik, J.; Chervenak, James A.

doi:10.1016/j.cryobiol.2010.10.036

Cited by 1 publication

(1 citation statement)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The tardigrades' ability to repair the double-strand break damage allows for survival not only in the deep cryo-environment but also under several orders of magnitude a larger radiation exposure dose than the dose that is lethal to humans. 29 , 30 , 67 In order to fix the DNA damage from double-strand breaks, the sugars must be either already present near the chromatin fiber, preventing the damaged strands from migrating out from their positions before breaks, thereby allowing the DNA repair mechanism to fix the double-strand brake damage.…”

Section: Discussionmentioning

confidence: 99%

Dissolved Gases and Ice Fracturing During the Freezing of a Multicellular Organism: Lessons from Tardigrades

Kletetschka

Hruba

2015

BioResearch Open Access

View full text Add to dashboard Cite

Three issues are critical for successful cryopreservation of multicellular material: gases dissolved in liquid, thermal conductivity of the tissue, and localization of microstructures. Here we show that heat distribution is controlled by the gas amount dissolved in liquids and that when changing the liquid into solid, the dissolved gases either form bubbles due to the absence of space in the lattice of solids and/or are migrated toward the concentrated salt and sugar solution at the cost of amount of heat required to be removed to complete a solid-state transition. These factors affect the heat distribution in the organs to be cryopreserved. We show that the gas concentration issue controls fracturing of ice when freezing. There are volumetric changes not only when changing the liquid into solid (volume increases) but also reduction of the volume when reaching lower temperatures (volume decreases). We discuss these issues parallel with observations of the cryosurvivability of multicellular organisms, tardigrades, and discuss their analogy for cryopreservation of large organs.

show abstract

Section: Discussionmentioning

confidence: 99%