32. Evaluation of a rapid chromatographic strip test for the pen-side detection of foot-and-mouth disease virus antigen

Reid, Scott M.; Ferris, N.P.; Hutchings, G.; Kowalska, Z; Danks, C.; Preston, Simon; Barker, Ian K.

doi:10.1016/s0034-5288(02)90034-2

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The differences in the strength of the reaction for individual isolates were due to differences in the affinities of the antibodies used in the two assays (polyclonal antibody versus. mAbs; Ferris et al, 2010 The World Reference Laboratory for Foot-and-Mouth Disease (WRLFMD) reported that 70%-80% of the positive specimens were diagnosed using DAS ELISAs from tissue suspensions of clinical material, the remaining 20%-30% of field samples were diagnosed positive after virus amplification in cell culture (Reid, Bruening, Ferris, Hutchings, & Akerblom, 2000). Virus amplification may require several days for completion and delay the implementation of correct control procedures.…”

Section: Serological Comparisons Of Fmdv Sat 2 Viruses From Kenyamentioning

confidence: 99%

Generation of monoclonal antibodies against foot‐and‐mouth disease virus SAT 2 and the development of a lateral flow strip test for virus detection

Yang

Mudabuka

Quizon

et al. 2018

Transbound Emerg Dis

View full text Add to dashboard Cite

Foot‐and‐mouth disease (FMD) remains a major economic concern for the livestock productivity in many developing countries and a continued threat to countries that are disease free because of its potential devastating impact on agricultural, food chain and tourism sectors. FMD virus (FMDV) is recognized as having seven serotypes: O, A, C, Asia 1, South African Territories (SAT) 1, 2, 3 and multiple subtypes within each serotype. FMD outbreaks due to SAT 2 have been reported in many African countries. The development of a rapid and easily performed test for FMD detection is critical for controlling FMD outbreaks and containing its spread. The present project developed a lateral flow immunochromatographic (LFI) strip test for the rapid detection of FMDV SAT 2. A panel of monoclonal antibodies (mAbs) against FMDV serotype SAT 2 was produced and characterized. One mAb (#10) was selected as the capture mAb because it reacted to all 23 SAT 2 isolates archived at the National Center for Foreign Animal Disease. The LFI strip test was developed using biotin‐conjugated mAb #10, and the colloid gold‐conjugated FMDV serotype‐independent mAb as the detection mAb. A generic Rapid Assay Device (gRAD) with one test line and a control line was used for the test. The LFI strip test detected all 23 tested SAT 2 isolates and recent outbreak strains. The results indicated that the diagnostic specificity and sensitivity of the LFI strip test were greater than the double antibody sandwich (DAS) DAS ELISA. The ability of the LFI strip test to produce rapid diagnostic results will be useful for early on‐site diagnosis during FMD outbreaks.

show abstract

Section: Serological Comparisons Of Fmdv Sat 2 Viruses From Kenyamentioning

confidence: 99%