31P-NMR spectroscopy of human liver and bile

Khristianovich, D. S.; Reshetnyak, Vasiliy Ivanovich; Loginov, A S; Yushmanov, Victor E.; Tumanyan, M. A.; Sibel'dina, L A

doi:10.1007/bf00802173

Cited by 8 publications

(7 citation statements)

References 3 publications

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“…Despite the advantages of in vitro 31 P NMR spectroscopy, there is surprisingly little available literature on 31 P NMR spectroscopy of human bile, compared with 1 H NMR spectroscopy. In a Russian study, Khristianovich and colleagues (65) showed that concentrations of PC and Pi were higher in gall bladder bile compared with bile from the liver, as would be expected, the main role of the gall bladder being to store (approximately 50 ml), acidify and concentrate bile. This is achieved by diffusion of water following net absorption of Na + , Cl − , Ca 2+ and HCO 3 − .…”

Section: In Vitro Nmr Studies Of Bilementioning

confidence: 67%

In vivo and in vitro nuclear magnetic resonance spectroscopy as a tool for investigating hepatobiliary disease: a review of ¹H and ³¹P MRS applications

Khan

Cox

Hamilton

et al. 2005

Liver International

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Nuclear magnetic resonance (NMR) spectroscopy is a non-invasive technique, which allows the study of cellular biochemistry and metabolism. It is a diverse research tool, widely used by biochemists to investigate pathophysiological processes in vitro and, more recently, by physicians to determine disease abnormalities in vivo. This article reviews the basics of the NMR phenomenon and summarises previous research on the hepatobiliary system using both laboratory-based and clinical methodologies. The role of proton and phosphorus-31 ((31)P) NMR spectroscopy in the study of malignant and non-malignant liver disease and studies of bile composition are discussed. In vivo techniques (magnetic resonance spectroscopy, MRS) can be performed as an adjunct to standard MR examination of the liver. Although still primarily a research tool, the in vivo technique provides non-invasive biochemical information on disease severity and holds promise in its use to gauge response to treatment regimens.

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Section: In Vitro Nmr Studies Of Bilementioning

confidence: 67%

In vivo and in vitro nuclear magnetic resonance spectroscopy as a tool for investigating hepatobiliary disease: a review of ¹H and ³¹P MRS applications

Khan

Cox

Hamilton

et al. 2005

Liver International

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“…Similarly, the biochemical analysis of the endoscopically sampled bile of PSC patients has shown decreased PtdC and bile salt concentrations, especially if the bile was sampled directly from the gallbladder 8,32 . The relevance of the bile sampling size was also proved by Khristianovich and colleagues, who found generally increased phosphocholine and P i concentrations in gallbladder bile compared to the bile from the liver 33 . This study also showed decreased phosphocholine and P i levels in the bile of patients with PBC compared to controls.…”

Section: Discussionmentioning

confidence: 92%

Concentration of Gallbladder Phosphatidylcholine in Cholangiopathies: A Phosphorus‐31 Magnetic Resonance Spectroscopy Pilot Study

Pfleger

Halilbasic

Gajdošík

et al. 2021

Magnetic Resonance Imaging

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Background: Biliary phosphatidylcholine (PtdC) concentration plays a role in the pathogenesis of bile duct diseases. In vivo phosphorus-31 magnetic resonance spectroscopy ( 31 P-MRS) at 7 T offers the possibility to assess this concentration noninvasively with high spectral resolution and signal intensity. Purpose: Comparison of PtdC levels of cholangiopathic patient groups to a control group using a measured T 1 relaxation time of PtdC in healthy subjects. Study Type: Case control. Subjects: Two patient groups with primary sclerosing cholangitis (PSC, 2f/3 m; age: 43 AE 7 years) and primary biliary cholangitis (PBC, 4f/2 m; age: 57 AE 6 years), and a healthy control group (CON, 2f/3 m; age: 38 AE 7 years). Ten healthy subjects for the assessment of the T 1 relaxation time of PtdC. Field Strength/Sequence: A 3D phase-encoded pulse-acquire 31 P-MRSI sequence for PtdC quantification and a 1D image-selected in vivo 31 P spectroscopy for T 1 estimation at 7 T, and a T2-weighted half-Fourier single-shot turbo spin echo MRI sequence for volumetry at 3 T. Assessment: Calculation of gallbladder volumes and PtdC concentration in groups using hepatic gamma-adenosine triphosphate signal as an internal reference and correction for insufficient relaxation of PtdC with a T 1 value assessed in healthy subjects. Statistical Tests: Group comparison of PtdC content and gallbladder volumes of the PSC/PBC and CON group using Student's t-tests with a significance level of 5%. Results: PtdC T 1 value of 357 AE 85 msec in the gallbladder. Significant lower PtdC content for the PSC group, and for the female subgroup of the PBC group compared to the CON group (PSC/CON: 5.74 AE 0.73 mM vs. 9.64 AE 0.97 mM, PBC(f)/ CON: 5.77 AE 1.44 mM vs. 9.64 AE 0.97 mM). Significant higher gallbladder volumes of the patient groups compared to the CON group (PSC/CON: 66.3 AE 15.8 mL vs. 20.9 AE 2.2 mL, PBC/CON: 49.8 AE 18.2 mL vs. 20.9 AE 2.2 mL).Data Conclusion: This study demonstrated the application of a 31 P-MRSI protocol for the quantification of PtdC in the human gallbladder at 7 T. Observed differences in PtdC concentration suggest that this metabolite could serve as a biomarker for specific hepatobiliary disorders. Level of Evidence: 2 Technical Efficacy Stage: 3

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“…A selective reduction in biliary phospholipids has been suggested to be responsible for cholesterol gallstones in certain populations [72] . Initial 31 P MRS studies on bile from patients with primary biliary cirrhosis have shown reduced levels of PTC and Pi when compared to bile from healthy volunteers [64] , but the significance of these findings still remains to be determined in more extensive studies.…”

Section: Mr Spectroscopy Of Bile In Diseasementioning

confidence: 98%

“…31 P NMR spectroscopy has been used to quantify phospholipids in red cell membranes and in model bile salts [63] . Such 31 P MRS studies have shown higher concentrations of PTC and Pi in gallbladder bile compared to canalicular bile [64] .…”

Section: Bile Physiology By Mrsmentioning

confidence: 99%

“…Biliary composition of these lipids also varies in cholestatic diseases of the liver and malignancy of the biliary tree. Current applications of MRS in biliary disease include assessment of bile content in cholesterol gallstones, rejection in liver transplantation, primary biliary cirrhosis and biliary tract malignancy, as well in biliary excretion of xenobiotics [64,[66][67][68][69] . MRS has been used to assess the distribution of biliary lipids between vesicles and micelles, which is believed to have a role in gallstone disease, and there is evidence that cholesterol from vesicular aggregates may be responsible for the deposition of cholesterol stones in the gallbladder [70] .…”

Section: Mr Spectroscopy Of Bile In Diseasementioning

confidence: 99%

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Current and future applications ofin vitromagnetic resonance spectroscopy in hepatobiliary disease

Cox

Sharif²,

Cobbold³

et al. 2006

WJG

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Nuclear magnetic resonance spectroscopy allows the study of cellular biochemistry and metabolism, both in the whole body in vivo and at higher magnetic field strengths in vitro . Since the technique is non-invasive and non-selective, magnetic resonance spectroscopy methodologies have been widely applied in biochemistry and medicine. In vitro magnetic resonance spectroscopy studies of cells, body fluids and tissues have been used in medical biochemistry to investigate pathophysiological processes and more recently, the technique has been used by physicians to determine disease abnormalities in vivo . This highlighted topic illustrates the potential of in vitro magnetic resonance spectroscopy in studying the hepatobiliary system. The role of in vitro proton and phosphorus magnetic resonance spectroscopy in the study of malignant and non-malignant liver disease and bile composition studies are discussed, particularly with reference to correlative in vivo whole-body magnetic resonance spectroscopy applications. In summary, magnetic resonance spectroscopy techniques can provide non-invasive biochemical information on disease severity and pointers to underlying pathophysiological processes. Magnetic resonance spectroscopy holds potential promise as a screening tool for disease biomarkers, as well as assessing therapeutic response.

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31P-NMR spectroscopy of human liver and bile

Cited by 8 publications

References 3 publications

In vivo and in vitro nuclear magnetic resonance spectroscopy as a tool for investigating hepatobiliary disease: a review of ¹H and ³¹P MRS applications

In vivo and in vitro nuclear magnetic resonance spectroscopy as a tool for investigating hepatobiliary disease: a review of ¹H and ³¹P MRS applications

Concentration of Gallbladder Phosphatidylcholine in Cholangiopathies: A Phosphorus‐31 Magnetic Resonance Spectroscopy Pilot Study

Current and future applications ofin vitromagnetic resonance spectroscopy in hepatobiliary disease

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31P-NMR spectroscopy of human liver and bile

Cited by 8 publications

References 3 publications

In vivo and in vitro nuclear magnetic resonance spectroscopy as a tool for investigating hepatobiliary disease: a review of 1H and 31P MRS applications

In vivo and in vitro nuclear magnetic resonance spectroscopy as a tool for investigating hepatobiliary disease: a review of 1H and 31P MRS applications

Concentration of Gallbladder Phosphatidylcholine in Cholangiopathies: A Phosphorus‐31 Magnetic Resonance Spectroscopy Pilot Study

Current and future applications ofin vitromagnetic resonance spectroscopy in hepatobiliary disease

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In vivo and in vitro nuclear magnetic resonance spectroscopy as a tool for investigating hepatobiliary disease: a review of ¹H and ³¹P MRS applications

In vivo and in vitro nuclear magnetic resonance spectroscopy as a tool for investigating hepatobiliary disease: a review of ¹H and ³¹P MRS applications