One hundred and ninety-one isolates were obtained from 321 cases consisting of tinea capitis 7 (2.2%), tinea corporis 86 (26.8%), tinea cruris 63 (19.6%), tinea pedis 140 (43.6%), tinea manuum 4 (1.2%) and tinea unguium 21 (6.5%). The isolates were 152 (79.6%) of Trichophyton rubrum, 16 (8.4%) of T. mentagrophytes, 3 (1.6%) of T. violaceum, 3 (1.6%) of Trichophyton spp., 14 (7.3%) of Microsporum canis and 3 (1.6%) of M. gypseum. Out of 152 T. rubrum isolates, 104 were granular or powdery, and urease-positive. The dermatophyte flora in Amami-Oshima was intermediate between those in Kyushu and Okinawa.