315.6: Assessment of Biliary Regeneration During Long Term (&gt;5 Days) Ex-vivo Normothermic Machine Perfusion

Ly, Mark; Lau, Ngee-Soon; Dennis, Claude; Huang, Joanna L; Toomath, Shamus; Cabanes‐Creus, Marti; McKenzie, Catriona; Kench, James G.; Majumdar, Avik; Gorrell, Mark D.; McCaughan, Geoffrey W.; Crawford, Michael H.; Pulitanò, Carlo

doi:10.1097/01.tp.0000886596.62496.8c

Cited by 3 publications

(3 citation statements)

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“…11,12 When long-term (>24 h) NMP becomes clinically available, the risk of microbial contamination may increase. 13 Lau et al 14 recently reported a high rate of bacterial contamination (50%) during long-term NMP in an experimental, preclinical setting. Although this percentage appears relatively high, and this may have resulted from contamination during ex situ splitting of the liver and from insufficient sterility precautions in a preclinical research setting, it indicates that more research is needed to assess the efficacy and optimal dosing of antimicrobial prophylaxis during long-term NMP.…”

Section: Discussionmentioning

confidence: 99%

The Risk of Microbial Transmission in Recipients of Donor Livers That Underwent Hypothermic or Normothermic Machine Perfusion

Endo,

Lascaris,

Brüggenwirth

et al. 2024

Transplantation Direct

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Background. Ex situ machine perfusion is increasingly used to preserve and assess donor livers before transplantation. Compared with traditional static cold storage (SCS), machine perfusion exposes livers to an additional risk of microbial contamination. However, information on the risk of microbial transmission during machine perfusion is lacking. Methods. All livers that underwent either hypothermic oxygenated machine perfusion (HOPE) or normothermic machine perfusion (NMP) in our center between September 2021 and September 2023, and during which samples were taken from SCS fluid and/or machine perfusion solution for microbiological examination, were included in this retrospective, observational clinical study. Microbial transmission was examined from SCS fluid to machine perfusion solution fluid and, subsequently, to recipients of these livers. Results. A total of 90 cases of liver machine perfusion were included: 59 HOPE and 31 NMP. SCS preservation fluid cultures before HOPE or NMP were positive for at least 1 microorganism in 52% of the cases. After HOPE, there were no cases of positive machine perfusion fluid or evidence of microbial transmission to the recipients. After NMP, in 1 (3%) patient Escherichia coli was grown from abdominal drain fluid, the same bacterial strain that was also grown from the SCS preservation fluid before NMP. This E coli was resistant to the antibiotics that are routinely added to the NMP perfusion fluid. Conclusions. The risk of microbial transmission after machine perfusion is very low but not absent. We recommend routine sampling of machine perfusion fluid at the end of the procedure for microbiological analysis.

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Section: Discussionmentioning

confidence: 99%

The Risk of Microbial Transmission in Recipients of Donor Livers That Underwent Hypothermic or Normothermic Machine Perfusion

Endo,

Lascaris,

Brüggenwirth

et al. 2024

Transplantation Direct

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“…31 Several groups presented their customized MP device model. Ly et al 2 developed a model for small animals (rats), allowing proper liver preservation for up to 5 d. The system was composed of an organ reservoir, a heat exchanger, a hollow fiber oxygenator, a peristaltic pump and a dialysis filter, and hormonal and nutritional support were continuously infused. Lascaris et al, 18 Clarke et al, 19 and Huang et al 9 demonstrated the fundamental role of a dialysis system associated with NMP in maintaining a physiological perfusate, avoiding electrolytes disturbances, and ultimately keeping livers alive for several days.…”

Section: Ischemia-reperfusion Injury and Machine Perfusionmentioning

confidence: 99%

What Is Hot and New in Basic and Translational Science in Liver Transplantation in 2023? Report of the Basic and Translational Research Committee of the International Liver Transplantation Society

Bonaccorsi-Riani,

Ghinolfi,

Czigany

et al. 2024

Transplantation

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The 2023 Joint Annual Congress of the International Liver Transplantation Society, European Liver and Intestine Transplant Association, and Liver Intensive Care Group of Europe were held in Rotterdam, the Netherlands, from May 3 to 6, 2023. This year, all speakers were invited to attend the Congress in person for the first time since the COVID-19 pandemic. The congress was attended by 1159 registered delegates from 54 countries representing 5 continents, with the 10 countries comprising the bulk of the delegates. Of the 647 abstracts initially submitted, 542 were eventually presented at the meeting, coming from 38 countries (mainly North America, Europe, and Asia) and 85% of them (462 abstracts) came from only 10 countries. Fifty-three (9.8%) abstracts, originated from 17 countries, were submitted under the Basic/Translational Scientific Research category, a similar percentage as in 2022. Abstracts presented at the meeting were classified as (1) ischemia and reperfusion injury, (2) machine perfusion, (3) bioengineering and liver regeneration, (4) transplant oncology, (5) novel biomarkers in liver transplantation, (6) liver immunology (rejection and tolerance), and (7) artificial intelligence and machine learning. Finally, we evaluated the number of abstracts commented in the Basic and Translational Research Committee-International Liver Transplantation Society annual reports over the past 5 y that resulted in publications in peer-reviewed journals to measure their scientific impact in the field of liver transplantation.

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“…As a result, the grafts were able to meet the viability criteria for as long as 13 d. 6 The same group showed that, after 72 h NMP, biliary reepithelialization could occur, and biliary viability criteria could be met even by grafts that did not meet them at the beginning of NMP. 7 As long as NMP can be prolonged, more opportunities for ex situ organ treatment could be achieved. Despite these promising prospects, a more thorough evaluation of the mechanisms of NMP is needed to better understand these findings and fully exploit the potential for NMP preservation, treatment, and reconditioning.…”

Section: Normothermic Machine Perfusionmentioning

confidence: 99%

What Is Hot and New in Basic and Translational Science in Liver Transplantation in 2022? Report of the Basic and Translational Research Committee of the International Liver Transplantation Society

et al. 2022

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315.6: Assessment of Biliary Regeneration During Long Term (>5 Days) Ex-vivo Normothermic Machine Perfusion

Cited by 3 publications

References 0 publications

The Risk of Microbial Transmission in Recipients of Donor Livers That Underwent Hypothermic or Normothermic Machine Perfusion

The Risk of Microbial Transmission in Recipients of Donor Livers That Underwent Hypothermic or Normothermic Machine Perfusion

What Is Hot and New in Basic and Translational Science in Liver Transplantation in 2023? Report of the Basic and Translational Research Committee of the International Liver Transplantation Society

What Is Hot and New in Basic and Translational Science in Liver Transplantation in 2022? Report of the Basic and Translational Research Committee of the International Liver Transplantation Society

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