[31] Immunoassays using acridinium esters

Weeks, Ian; Sturgess, M.; Rc, Brown; Js, Woodhead

doi:10.1016/0076-6879(86)33080-5

Cited by 73 publications

(34 citation statements)

References 18 publications

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“…Tagging antibodies with fluorescent molecules has also been shown to be a sensitive method of detection (Weeks et al, 1986). A most useful assay, using fluorescence-labelled antibodies, which may well be adopted by plant pathologists, is the time-resolved fluoroimmunoassay (TR-FIA) (Halonen et al, 1986).…”

Section: Serological Methodsmentioning

confidence: 99%

New Approaches to the Detection of Microbial Plant Pathogens

Chu

Waterhouse

Martín

et al. 1989

Biotechnology and Genetic Engineering Reviews

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Section: Serological Methodsmentioning

confidence: 99%

New Approaches to the Detection of Microbial Plant Pathogens

Chu

Waterhouse

Martín

et al. 1989

Biotechnology and Genetic Engineering Reviews

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“…Acridinium salts and related compounds, owing to their highly chemiluminescence (CL) quantum yield and rapid CL reaction, proved to be useful CL labels with the advantages of high stability, specificity and sensitivity, which exceeded those of radioisotopes [1][2][3]. They were extremely useful labels that have been used extensively in immunoassays [1][2][3][4][5][6][7][8] and nucleic acid assays [9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

“…They were extremely useful labels that have been used extensively in immunoassays [1][2][3][4][5][6][7][8] and nucleic acid assays [9-13].…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis chemiluminescent detection system equipped with a two‐step postcolumn flow interface for detection of some enkephalin‐related peptides labeled with acridinium ester

et al. 2008

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Despite its low equipment cost and simple design, as one of the sensitive detectors for CE, the chemiluminescence (CL) detector was less developed compared to the detectors of MS and LIF. The main reasons were the limitation of CL reagents, the repeatability problems and the relatively low sensitivity compared to LIF. In this paper, a highly sensitive CE-CL detection system was developed for detection of some enkephalin-related peptides labeled with acridinium ester. A new detection interface was designed for CE with CL detection of acridinium ester and its labeled analytes. The interface included two sections: one was used to acidify the capillary outflow so that the corresponding acridinium pseudo-base form can be changed into acridinium ester form by adding excess acid to the system; the other was designed to provide a suitable solution to produce the CL from acridinium ester. The effect factors, such as pH, the concentration of reaction reagents and the flow rates of the reagents, were investigated. The results showed that acridinium ester had similar CL properties in this interface when pH values of CE BGE were changed from 2.0 to 10.8. The interface was used to detect acridinium ester and three acridinium ester-labeled enkephalin-related peptides, the corresponding LODs were found to be in the attomole range. This CL detection system proved to be of high sensitivity, good repeatability, and relatively low cost.

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“…Expressed simply, the chemical entities used to generate the chemiluminescent signal can be divided into two categories: those that result in a relatively short-lived (seconds) but intense signal (flash type) and those which produce a longer-lived signal (minutes or hours), generally of a lesser intensity (glow type). Of the first category (flash type) two classes of compound have had the greatest application, namely acridinium esters (AEs) 2 and luminol (e.g., aminobutylethylisoluminol). Here chemiluminescence is initiated by the addition of oxidant, usually hydrogen peroxide, at high alkaline pH either with (luminol-type compounds) or without (AEs) a catalyst.…”

mentioning

confidence: 99%