[31] High-performance liquid chromatography-coulometric electrochemical detection of ubiquinol 10, ubiquinone 10, carotenoids, and tocopherols in neonatal plasma

Finckh, Barbara; Kontush, Anatol; Commentz, J C; Hübner, Christoph; Burdelski, M.; Kohlschütter, Alfried

doi:10.1016/s0076-6879(99)99034-1

Cited by 47 publications

(28 citation statements)

References 17 publications

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“…We suggest tography (HPLC) has been reported (14)(15)(16)(17)(18)(19)(20)(21)). An electrothat this method will be useful since the ratio of ubichemical detector (ECD) 2 is preferred for detection of quinol to ubiquinone has been suggested as a good ubiquinol due to its high sensitivity (14)(15)(16)(17)(18)(19)(20)(21)(22).…”

mentioning

confidence: 84%

“…᭧ 1997 Academic Press none is insensitive to ECD in its oxidized form and is detected by its uv absorption at 275 nm (14)(15)(16)(17)(18). However, on-line reduction of ubiquinone to ubiquinol using Ubiquinol has received renewed attention since it a reduction column (19,20) or by a coulometric method has been recognized to possess a good antioxidant ac- (21,22) has been recently reported which allows for the tivity (1)(2)(3)(4)(5)(6)(7)(8)(9). In this capacity, it can inhibit lipid peroxi-sensitive detection of ubiquinone using ECD.…”

mentioning

confidence: 99%

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Simultaneous Detection of Ubiquinol and Ubiquinone in Human Plasma as a Marker of Oxidative Stress

Yamashita

Yamamoto

1997

Analytical Biochemistry

202

129

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mentioning

confidence: 84%

mentioning

confidence: 99%

Simultaneous Detection of Ubiquinol and Ubiquinone in Human Plasma as a Marker of Oxidative Stress

Yamashita

Yamamoto

1997

Analytical Biochemistry

202

129

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“…The concentration of ascorbate was measured by reversed-phase HPLC with colorimetric detection (31). The concentration of ␣-tocopherol in plasma samples was measured by HPLC with amperometric detection (18).…”

Section: Methodsmentioning

confidence: 99%

Effect of antioxidant supplementation on insulin sensitivity in response to endurance exercise training

Yfanti

Nielsen

Åkerström

et al. 2011

American Journal of Physiology-Endocrinology and Metabolism

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production of reactive oxygen and nitrogen species (RONS) is associated with some of the beneficial adaptations to regular physical exercise, it is not established whether RONS play a role in the improved insulin-stimulated glucose uptake in skeletal muscle obtained by endurance training. To assess the effect of antioxidant supplementation during endurance training on insulin-stimulated glucose uptake, 21 young healthy (age 29 Ϯ 1 y, BMI 25 Ϯ 3 kg/m 2 ) men were randomly assigned to either an antioxidant [AO; 500 mg vitamin C and 400 IU vitamin E (␣-tocopherol) daily] or a placebo (PL) group that both underwent a supervised intense endurance-training program 5 times/wk for 12 wk. A 3-h euglycemic-hyperinsulinemic clamp, a maximal oxygen consumption (V O2max) and maximal power output (Pmax) test, and body composition measurements (fat mass, fat-free mass) were performed before and after the training. Muscle biopsies were obtained for determination of the concentration and activity of proteins regulating glucose metabolism. Although plasma levels of vitamin C (P Ͻ 0.05) and ␣-tocopherol (P Ͻ 0.05) increased markedly in the AO group, insulin-stimulated glucose uptake increased similarly in both the AO (17.2%, P Ͻ 0.05) and the PL (18.9%, P Ͻ 0.05) group in response to training. V O2max and Pmax also increased similarly in both groups (time effect, P Ͻ 0.0001 for both) as well as protein content of GLUT4, hexokinase II, and total Akt (time effect, P Յ 0.05 for all). Our results indicate that administration of antioxidants during strenuous endurance training has no effect on the training-induced increase in insulin sensitivity in healthy individuals.

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“…The standard solutions were stored sealed under argon at Ϫ20°C. The concentrations were calculated every month using known extinction coefficients for the ubiquinones, ubiquinols, and tocopherols (23).…”

Section: Figmentioning

confidence: 99%

Simultaneous Detection of Ubiquinol-10, Ubiquinone-10, and Tocopherols in Human Plasma Microsamples and Macrosamples as a Marker of Oxidative Damage in Neonates and Infants

Menke

Niklowitz

Adam³

et al. 2000

Analytical Biochemistry

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[31] High-performance liquid chromatography-coulometric electrochemical detection of ubiquinol 10, ubiquinone 10, carotenoids, and tocopherols in neonatal plasma

Cited by 47 publications

References 17 publications

Simultaneous Detection of Ubiquinol and Ubiquinone in Human Plasma as a Marker of Oxidative Stress

Simultaneous Detection of Ubiquinol and Ubiquinone in Human Plasma as a Marker of Oxidative Stress

Effect of antioxidant supplementation on insulin sensitivity in response to endurance exercise training

Simultaneous Detection of Ubiquinol-10, Ubiquinone-10, and Tocopherols in Human Plasma Microsamples and Macrosamples as a Marker of Oxidative Damage in Neonates and Infants

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