[31] Direct current electrical measurement in epithelia: Steady-state and transient analysis

Rehm, Warren S.; Schwartz, Manuel; Carrasquer, Gaspar

doi:10.1016/s0076-6879(89)71034-x

Cited by 2 publications

(7 citation statements)

References 18 publications

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“…In mounted mucosae, very strong pump inhibition has been seen to increase transmucosal resistance, but PPIs are not known to inhibit any ion channels (23) and the increased resistance is thought to result from decreased gland luminal diameter (not modeled here) at very low secretory rates (43,44). Extended daily omeprazole dosing in adult rabbits gradually increased permeability of gastric secretory membranes to H ϩ (3), but only the simpler case of acute inhibition with no change in H ϩ permeability is modeled here.…”

Section: Resultsmentioning

confidence: 99%

“…is used for membrane voltages, with resistances of other cell types and tight junctions considered high enough to be ignored (43,50). An Ohm's-law equation is used for ion fluxes: Eq/s ϭ G ion ·(V m ϪE ion )/F.…”

Section: Construction Of the Parietal Cell Modelmentioning

confidence: 99%

“…With experimental short-circuiting (SC) of a gastric mucosa, there is a slow initial change in applied current, lasting many seconds, rather than milliseconds, called the "long timeconstant transient" (22,43). Modeled SC in the resting state rapidly hyperpolarizes apical V m (Fig.…”

Section: T Less Mucosal-negative Than Open-circuit V T Increases Clmentioning

confidence: 99%

“…The very beginning of SC is the only point at which V t is driven to zero solely by the applied current. With the use of ⌬V t and ⌬I from the model to calculate resting R t , 35 (43).…”

Section: T Less Mucosal-negative Than Open-circuit V T Increases Clmentioning

confidence: 99%

“…conductance of the acid-secreting mucosa (43) and that tight junctions are very "tight" (50), as they can sustain a millionfold ⌬[H ϩ ] in the stimulated state. Kidder and Rehm (22) used only Ϫ10 A with frog mucosa to cause the initial ⌬V t of 2 mV, which required Ϫ41 A in our model.…”

Section: T Less Mucosal-negative Than Open-circuit V T Increases Clmentioning

confidence: 99%

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Computer modeling of gastric parietal cell: significance of canalicular space, gland lumen, and variable canalicular [K⁺]

Crothers

Forte

Machen

2016

American Journal of Physiology-Gastrointestinal and Liver Physi

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Section: Resultsmentioning

confidence: 99%

Section: Construction Of the Parietal Cell Modelmentioning

confidence: 99%

Section: T Less Mucosal-negative Than Open-circuit V T Increases Clmentioning

confidence: 99%

“…The very beginning of SC is the only point at which V t is driven to zero solely by the applied current. With the use of ⌬V t and ⌬I from the model to calculate resting R t , 35 (43).…”

Section: T Less Mucosal-negative Than Open-circuit V T Increases Clmentioning

confidence: 99%

Section: T Less Mucosal-negative Than Open-circuit V T Increases Clmentioning

confidence: 99%

See 3 more Smart Citations

Computer modeling of gastric parietal cell: significance of canalicular space, gland lumen, and variable canalicular [K⁺]

Crothers

Forte

Machen

2016

American Journal of Physiology-Gastrointestinal and Liver Physi

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Claudin-18 Loss Alters Transcellular Chloride Flux but not Tight Junction Ion Selectivity in Gastric Epithelial Cells

Caron

Scott

Sinha

et al. 2021

Cellular and Molecular Gastroenterology and Hepatology

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Claudin-18 loss during Helicobacter pylori infection promotes neoplastic progression. This phenotype was not due to barrier dysfunction, because paracellular permeability was not affected by claudin-18 deletion but may be secondary to transcellular anion transporter expression/function in the absence of claudin-18. BACKGROUND & AIMS: Tight junctions form a barrier to the paracellular passage of luminal antigens. Although most tight junction proteins reside within the apical tight junction complex, claudin-18 localizes mainly to the basolateral membrane where its contribution to paracellular ion transport is undefined. Claudin-18 loss in mice results in gastric neoplasia development and tumorigenesis that may or may not be due to tight junction dysfunction. The aim here was to investigate paracellular permeability defects in stomach mucosa from claudin-18 knockout (Cldn18-KO) mice. METHODS: Stomach tissue from wild-type, heterozygous, or Cldn18-KO mice were stripped of the external muscle layer and mounted in Ussing chambers. Transepithelial resistance, dextran 4 kDa flux, and potential difference (PD) were calculated from the chambered tissues after identifying differences in tissue histopathology that were used to normalize these measurements. Marker expression for claudins and ion transporters were investigated by transcriptomic and immunostaining analysis. RESULTS: No paracellular permeability defects were evident in stomach mucosa from Cldn18-KO mice. RNAseq identified changes in 4 claudins from Cldn18-KO mice, particularly the upregulation of claudin-2. Although claudin-2 localized to tight junctions in cells at the base of gastric glands, its presence did not contribute overall to mucosal permeability. Stomach tissue from Cldn18-KO mice also had no PD versus a lumen-negative PD in tissues from wild-type mice. This difference resulted from changes in transcellular Clpermeability with the downregulation of Clloading and Clsecreting anion transporters. CONCLUSIONS: Our findings suggest that Cldn18-KO has no effect on tight junction permeability in the stomach from adult mice but rather affects anion permeability. The phenotype in these mice may thus be secondary to transcellular anion transporter expression/function in the absence of claudin-18.

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[31] Direct current electrical measurement in epithelia: Steady-state and transient analysis

Cited by 2 publications

References 18 publications

Computer modeling of gastric parietal cell: significance of canalicular space, gland lumen, and variable canalicular [K⁺]

Computer modeling of gastric parietal cell: significance of canalicular space, gland lumen, and variable canalicular [K⁺]

Claudin-18 Loss Alters Transcellular Chloride Flux but not Tight Junction Ion Selectivity in Gastric Epithelial Cells

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[31] Direct current electrical measurement in epithelia: Steady-state and transient analysis

Cited by 2 publications

References 18 publications

Computer modeling of gastric parietal cell: significance of canalicular space, gland lumen, and variable canalicular [K+]

Computer modeling of gastric parietal cell: significance of canalicular space, gland lumen, and variable canalicular [K+]

Claudin-18 Loss Alters Transcellular Chloride Flux but not Tight Junction Ion Selectivity in Gastric Epithelial Cells

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Computer modeling of gastric parietal cell: significance of canalicular space, gland lumen, and variable canalicular [K⁺]

Computer modeling of gastric parietal cell: significance of canalicular space, gland lumen, and variable canalicular [K⁺]