306 Immunohistochemical evidence of vanilloid receptor 1 (VR1) in normal human bladder urothelium

Lazzeri, Massimo; Turini, Damiano; Beneforti, P.; Spinelli, M.; Malaguti, S.; Vannucchi, Giulia; Zardo, Claudio; Faussone, M.S. Pellegrini

doi:10.1016/s1569-9056(04)90305-3

Cited by 4 publications

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“…Moreover, in situ hybridisation showing the presence of TRPV1 mRNA in the parietal cells and neurons confirmed that these cells express this receptor. In addition, Western blot analysis confirmed the presence of the TRPV1 in the human gastric mucosa since we found a band at about 97 kDa similar to that reported in the rat stomach and in the human urinary bladder (Yiangou 2001;Lazzeri et al 2004). However, at variance with other organs and/or animal species, in the human stomach, we found additional bands at a very low molecular weight that had not been previously described.…”

Section: Discussionsupporting

confidence: 88%

“…2d). This particular sub-cellular location has not been previously reported for any other cell type apart for human urothelium (Lazzeri et al 2004). A variety of sub-cellular TRPV1 locations has been described (Jahnel et al 2001;Gunthorpe et al 2002).…”

Section: Discussionsupporting

confidence: 52%

“…Calibration bar: a and d= 10 lm; b and c= 15 lm Kato et al (2003) in a gastric epithelial cell (RGM-1) line. Contradictory results on which cell type does express TRPV1 have been reported in a variety of organs of different mammals (Biro et al 1998;Nozawa et al 2001;Sanchez et al 2001;Lazzeri et al 2004). These discrepancies could be related to the different affinities of the antibodies used, some of which were selfmade and others, although commercially available, were purchased by different providers or rose in different animal species.…”

Section: Discussionmentioning

confidence: 83%

“…Epithelial location of TRPV1 has been previously shown in humans in the urothelium of both ureter and bladder (Lazzeri et al 2004). As well as for the stomach, a protective function of the TRPV1 might be envisaged for the lower urinary tract.…”

Section: Discussionmentioning

confidence: 90%

“…Immunohistochemical studies showed that TRPV1 is also expressed in several non-neuronal cell types of laboratory mammals (Nozawa et al 2001;Gunthorpe et al 2002) and, in humans, in the in situ urothelial cells of both ureter and urinary bladder (Lazzeri et al 2004). In the rat stomach, this receptor has been found in the mucous-secreting epithelial cells (Nozawa et al 2001) and in a cell line of gastric epithelium and, for, this reason it has been considered involved in gastroprotection at the epithelial level.…”

Section: Introductionmentioning

confidence: 99%

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Distribution of the vanilloid (capsaicin) receptor type 1 in the human stomach

Faussone–Pellegrini

Taddei

Bizzoco

et al. 2005

Histochem Cell Biol

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Vanilloid receptor type 1 (TRPV1) is expressed in a capsaicin-sensitive and peptide-containing sub-population of primary sensory nerves that in the rat stomach seems involved in regulation of chlorhydropeptic secretion and gastroprotection. Our aim was to identify which cell types express TRPV1 in the human stomach in order to gain a better insight in the role of this receptor in the regulation of HCl secretion. Immunohistochemistry, by using three different commercially available anti-capsaicin antibodies, in situ hybridisation and Western blot analysis were performed on fragments surgically obtained from the gastric body on the large curvature. TRPV1 labelling was found in the parietal cells at the level of intra-cytoplasmatic granules matching mitochondrial features and distribution. Immunolabelled neurons and nerve fibres were also seen, the latter numerous in the submucosa and mucosa and often ending close to the parietal cells. TRPV1 presence was confirmed by Western blot analysis and in situ hybridisation. TRPV1 presence in nerve structures and parietal cells suggests the possibility of a combined effect of both neuronal and epithelial TRPV1 on chlorhydropeptic secretion. The presumed TRPV1 mitochondrial location inside parietal cells is in favour of the existence of a local pathway of auto-regulation of HCl secretion. Therefore, TRPV1 might modulate chlorhydropeptic secretion in the human stomach through more complex pathways than previously thought.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Distribution of the vanilloid (capsaicin) receptor type 1 in the human stomach

Faussone–Pellegrini

Taddei

Bizzoco

et al. 2005

Histochem Cell Biol

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The Distribution of Sensory Fibers Immunoreactive for the TRPV1 (Capsaicin) Receptor in the Human Prostate

et al. 2005

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Recent Advances in Intravesical Drug/Gene Delivery

et al. 2006

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Targeting of the drugs administered systemically relies on the higher affinity of ligands for specific receptors to obtain selectivity in drug response. However, achieving the same goal inside the bladder is much easier with an intelligent pharmaceutical approach that restricts drug effects by exploiting the pelvic anatomical architecture of the human body. This regional therapy involves placement of drugs directly into the bladder through a urethral catheter. It is obvious that drug administration by this route holds advantage in chemotherapy of superficial bladder cancer and it has now become the most widely used treatment modality for this ailment. In recent years, the intravesical route has also been exploited either as an adjunct to an oral regimen or as a second-line treatment for neurogenic bladder 1, 2 . Instillation of DNA via this route using different vectors has been able to restrict the transgene expression in organs other than bladder. The present review article will discuss the shortcomings of the current options available for intravesical drug delivery (IDD) and lay a perspective for future developments in this field.

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306 Immunohistochemical evidence of vanilloid receptor 1 (VR1) in normal human bladder urothelium

Cited by 4 publications

References 0 publications

Distribution of the vanilloid (capsaicin) receptor type 1 in the human stomach

Distribution of the vanilloid (capsaicin) receptor type 1 in the human stomach

The Distribution of Sensory Fibers Immunoreactive for the TRPV1 (Capsaicin) Receptor in the Human Prostate

Recent Advances in Intravesical Drug/Gene Delivery

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