Can cadaverous pollution from environmental lead misguide to false positive results in the histochemical determination of Gunshot Residues? In-depth study using ultra-sensitive ICP-MS analysis on cadaveric skin samples

Boracchi, Michele; Andreola, Salvatore; Collini, Federica; Gentile, Guendalina; Lucchini, Giorgio; Maciocco, Francesca; Sacchi, Gian Attilio; Zoja, Riccardo

doi:10.1016/j.forsciint.2018.08.041

Cited by 5 publications

(5 citation statements)

References 9 publications

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“…Sampled tissues were then digested by using 65% HNO 3 at 120 • C. The mineralized samples were transferred into polypropylene test tubes. Samples were diluted 1:40 in MILLI-Q water and the concentration of metals elements was measured by Inductively Coupled Plasma-Mass Spectrometry ICP-MS (BRUKER Aurora-M90 ICP-MS) as previously described by Vigani et al (2017) and Barocchi et al (2018). Root Cu (II) Reductase and Fe (III) Chelate Reductase Activities Cu (II) and Fe (III) chelate reductases activities were determined on the root surface by use of 7 days-old intact roots seedlings grown in sterile plates exposed to either GMF or NNMF.…”

Section: Metals Content Determinationmentioning

confidence: 99%

The Geomagnetic Field Is a Contributing Factor for an Efficient Iron Uptake in Arabidopsis thaliana

2020

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The Earth's magnetic field, defined as the geomagnetic field (GMF), is an unavoidable environmental factor for all living organisms. Variation in the GMF intensity was found to affect the content of some nutrients and their associated channels and transporters in Arabidopsis thaliana. In this work, we observed that reduction of the GMF to near null magnetic field (NNMF) affects the accumulation of metals in plant tissues, mainly iron (Fe) and zinc (Zn) content, while the content of others metals such as copper (Cu) and manganese (Mn) is not affected. Accordingly, Fe uptake genes were induced in the roots of NNMF-exposed plants and the root Fe reductase activity was affected by transferring GMF-exposed plant to NNMF condition. Under Fe deficiency, NNMF-exposed plants displayed a limitation in the activation of Fe-deficiency induced genes. Such an effect was associated with the strong accumulation of Zn and Cu observed under NNMF conditions. Overall, our results provide evidence on the important role of the GMF on the iron uptake efficiency of plants.

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Section: Metals Content Determinationmentioning

confidence: 99%

The Geomagnetic Field Is a Contributing Factor for an Efficient Iron Uptake in Arabidopsis thaliana

2020

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“…Groups A and B and negative control presented values close to zero or very low being lower than the body values of the equipment. The positive control group presented high levels of Pb (2.618 and 82.97 μg/L), Ba (0.049 and 1.253 μg/L), and Sb 0.018 and 2.169 μg/L (34).…”

Section: Resultsmentioning

confidence: 99%

Detection of Pb, Ba, and Sb in Cadaveric Maggots and Pupae by ICP‐MS*

Costa¹,

Santos

Corrêa

et al. 2020

Journal of Forensic Sciences

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The concentrations of lead (Pb), barium (Ba), and antimony (Sb), characteristic of GSR, were determined in soil sediments and immature (larvae) of cadaveric flies of the family Calliphoridae, by inductively coupled plasma mass spectrometry (ICP-MS). This research refers to a case study from two real crime scenes in which the corpses were in an advanced state of decomposition. In case 1, the victim had holes similar to gunshot wounds, and in case 2, there was no evidence of perforations in the corpse. Soil sediment collection was performed at three different points of the terrain, at a minimum distance of 10 m from the corpse, for cases 1 and 2. In relation to the collection of immatures, larvae were collected in regions of the mouth, nose, and orifices similar to the entry of firearms projectile into the body, for case 1, and collection of larvae and pupae, located on the body and underneath it, for case 2. It was possible to detect and quantify the three elements of interest (Pb, Ba, and Sb) by ICP-MS in both sediment and cadaveric larvae. Concentrations of 4.44, 8.74, and 0.08 μg/g were obtained for Pb, Ba, and Sb, respectively, in the soil for case 1. For the case 2, the concentrations in Pb, Ba, and Sb were from 16.34 to 26.02 μg/g; from 32.64 to 57.97 μg/g and from 0.042 to 0.30 μg/g, respectively. In the larvae, Pb, Ba, and Sb were quantified in cases 1 and 2 with a concentration of 6.28 and 1.78 μg/g for Pb, 1.49 and 2.94 μg/g for Ba, 0.50 μg/g and

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“…We (11)(12)(13)(14)(15)(16)(17). In addition, the quality of the sample, the method of sampling and the expertise level of the sample collector also influence real-time RT-PCR test results (18).…”

Section: Discussionmentioning

confidence: 99%

“…Nineteen (4.67%) patients had positive RT-PCR test results 5 to 14 days later. Current available evidence suggests that several reasons contributed to the phenomenon of recovered patients with redetectable positive RT-PCR test results, including (a) genetic material contamination; (b) the use of different commercial kits; (c) sampling error involving throat swabs; (d) virus remaining in discharged patients; and (e) possible intermittent detoxification in some discharged patients ( 11 – 17 ). In addition, the quality of the sample, the method of sampling and the expertise level of the sample collector also influence real-time RT-PCR test results ( 18 ).…”

Section: Discussionmentioning

confidence: 99%

A Prediction Model for High Risk of Positive RT-PCR Test Results in COVID-19 Patients Discharged From Wuhan Leishenshan Hospital, China

Qian

Zeng

Pan

et al. 2021

Front. Public Health

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Several recent studies have reported that a few patients had positive SARS-CoV-2 RNA tests after hospital discharge. The high-risk factors associated with these patients remain to be identified. A total of 463 patients with COVID-19 discharged from Leishenshan Hospital in Wuhan, China, between February 8 and March 8, 2020 were initially enrolled, and 351 patients with at least 2 weeks of follow-up were finally included. Seventeen of the 351 discharged patients had positive tests for SARS-CoV-2 RNA. Based on clinical characteristics and mathematical modeling, patients with shorter hospital stays and less oxygen desaturation were at higher risk of SARS-CoV-2 RNA reoccurrence after discharge. Notably, traditional Chinese medicine treatment offered extensive benefits to reduce risk. Particular attention should be paid to those patients with high risk, and traditional Chinese medicine should be advocated.

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Can cadaverous pollution from environmental lead misguide to false positive results in the histochemical determination of Gunshot Residues? In-depth study using ultra-sensitive ICP-MS analysis on cadaveric skin samples

Cited by 5 publications

References 9 publications

The Geomagnetic Field Is a Contributing Factor for an Efficient Iron Uptake in Arabidopsis thaliana

The Geomagnetic Field Is a Contributing Factor for an Efficient Iron Uptake in Arabidopsis thaliana

Detection of Pb, Ba, and Sb in Cadaveric Maggots and Pupae by ICP‐MS*

A Prediction Model for High Risk of Positive RT-PCR Test Results in COVID-19 Patients Discharged From Wuhan Leishenshan Hospital, China

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