Molecular phylogeography and genetic diversity of Angiostrongylus cantonensis and A. malaysiensis (Nematoda: Angiostrongylidae) based on 66-kDa protein gene

Eamsobhana, Praphathip; Yong, Hoi Sen; Song, Sze Looi; Gan, Xiao Xian; Prasartvit, Anchana; Tungtrongchitr, Anchalee

doi:10.1016/j.parint.2018.09.006

Cited by 9 publications

(24 citation statements)

References 32 publications

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“…The more frequently found haplotypes (H1, H4, H7, H9, H16, H23, H28, and H41) are distributed in multiple populations in the four continents, and they come from different hosts. This differs from other studies performed with parasites like Corynosoma australe [ 25 ] (cytochrome c oxidase subunit I ( cox 1 ) sequences), Angiostrongylus cantonensis and A. malaysiensis [ 26 ] (partial 66-kDa protein gene sequence), or Plasmodium knowlesi [ 27 ] ( cox-1 and 18S RNA sequences), which showed haplogroups defined in the network and with geographic congruence. However, these studies used DNA fragments shorter than the data used in our present study.…”

Section: Discussioncontrasting

confidence: 96%

“…This pattern is commonly reported for other taxa (e.g. Psittacanthus calyculatus , canine distemper virus , Corynosoma austral , Fasciola hepatica ) [ 24 – 26 , 59 ]. Our results showed low structure and high genetic flow among domestic and wildlife hosts, and among populations worldwide.…”

Section: Discussionsupporting

confidence: 71%

“…1 ). However, haplogroup structure was not geographically congruent nor did it fit previous hypotheses proposed in the past [ 25 , 26 ]. The more frequently found haplotypes (H1, H4, H7, H9, H16, H23, H28, and H41) are distributed in multiple populations in the four continents, and they come from different hosts.…”

Section: Discussioncontrasting

confidence: 54%

“…Mutation rates and speed of selection differ among taxa, and in many cases, their dispersion to new areas is mediated by anthropogenic factors such as the mobility of the hosts towards different geographic regions, as has been shown by several studies (i.e. Vibrio vulnificus [ 21 ], canine distemper virus [ 24 ], Corynosoma australe [ 25 ], Angiostrongylus cantonensis , and A. malaysiensis [ 26 ]) including those performed with some apicomplexans ( Plasmodium knowlesi [ 27 ] and Toxoplasma gondi [ 28 ]).…”

Section: Introductionmentioning

confidence: 99%

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Phylogeography and population differentiation in Hepatozoon canis (Apicomplexa: Hepatozoidae) reveal expansion and gene flow in world populations

et al. 2021

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Background Hepatozoon canis is a protozoan transmitted to dogs and other wild carnivores by the ingestion of ticks containing mature oocysts and is considered the principal cause of canine hepatozoonosis in the world. Here, we examined ribosomal RNA 18S gene sequence variation to determine the genetic differences and phylogeographic diversity of H. canis from various geographical areas around the world. Methods We used 550 publicly available sequences of H. canis from 46 countries to assess haplotype relationships, geographical structure, genetic diversity indices, and relationships among populations. We performed neutrality tests and pairwise comparisons of fixation index (FST) values between groups and pairwise comparisons of FST values between populations. To determine whether populations are structured, analyses of molecular variance (AMOVAs) and spatial analysis of molecular variance (SAMOVA) were performed. Results The dataset of H. canis yielded 76 haplotypes. Differentiation among populations indicated that there is no phylogeographical structure (GST = 0.302 ± 0.0475). Moreover, when samples were grouped by continents a significant FST was obtained, meaning that populations were genetically differentiated. The AMOVA showed that 57.4% of the genetic variation was explained by differences within populations when all locations were treated as a single group and revealed that there is no population structure when populations are grouped into two, three, and four groups (FCT, p > 0.05), suggesting that dispersal between populations is high. SAMOVA revealed significant FCT values for groups K = 5. The Tajima’s D and Fu’s Fs show that populations have undergone recent expansion, and the mismatch distribution analysis showed population expansion (multimodal distribution). Conclusions The current molecular data confirmed that H. canis does not show phylogeographic or population structure. The haplotypes exhibit low genetic differentiation, suggesting a recent expansion due to gene flow among populations. These results provide pivotal information required for future detailed population genetic analysis or to establish control strategies of this parasite. Graphical abstract

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Section: Discussioncontrasting

confidence: 96%

Section: Discussionsupporting

confidence: 71%

Section: Discussioncontrasting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

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Phylogeography and population differentiation in Hepatozoon canis (Apicomplexa: Hepatozoidae) reveal expansion and gene flow in world populations

et al. 2021

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“…Several genes or nucleotide regions from A . cantonensis have been used in genetic studies so far: 66-kDa protein [21], ribosomal transcribed spacer (ITS) regions [22,23], small subunit (SSU) ribosomal RNA (18S rRNA) [22,24,25], cytochrome c oxidase subunit I (COI) [9,26], and cytochrome b ( cytb ) [27,28]. In comparison, only a few studies have tried to characterize A .…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of Angiostrongylus larvae and their intermediate host, Achatina fulica, in Thailand

et al. 2019

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Angiostrongyliasis is a parasitic disease caused by nematodes of the genus Angiostrongylus. Distribution of this worm corresponds to the dispersal of its main intermediate host, the giant African land snail Achatina fulica. Genetic characterization can help identify parasitic pathogens and control the spreading of disease. The present study describes infection of A. fulica by Angiostrongylus, and provides a genetic outlook based on sequencing of specific regions. We collected 343 land snails from 22 provinces across six regions of Thailand between May 2017 and July 2018. Artificial digestion and Baermann’s technique were employed to isolate Angiostrongylus larvae. The worm and its intermediate host were identified by sequencing with specific nucleotide regions. Phylogenetic tree was constructed to evaluate the relationship with other isolates. A. fulica from Chaiyaphum province was infected with A. cantonensis, whereas snails collected from Phrae and Chiang Rai provinces were infected with A. malaysiensis. The maximum likelihood tree based on 74 A. fulica COI sequences revealed monophyletic groups and identified two haplotypes: AF1 and AF2. Only AF1, which is distributed in all regions of Thailand, harbored the larvae of A. cantonensis and A. malaysiensis. Two mitochondrial genes (COI and cytb) and two nuclear regions (ITS2 and SSU rRNA) were sequenced in 41 Angiostrongylus specimens. The COI gene indicated that A. cantonensis was closely related to the AC10 haplotype; whereas the cytb gene revealed two new haplotypes: AC19 and AC20. SSU rRNA was useful for the identification of A. cantonensis; whereas ITS2 was a good genetic marker for differentiating between A. cantonensis and A. malaysiensis. This study provides genetic information about the parasite Angiostrongylus and its snail intermediate host. The data in this work may be useful for further study on the identification of Angiostrongylus spp., the genetic relationship between intermediate host and parasite, and control of parasites.

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Identification and genetic characterization of Angiostrongylus cantonensis isolated from the human eye

et al. 2023

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Molecular phylogeography and genetic diversity of Angiostrongylus cantonensis and A. malaysiensis (Nematoda: Angiostrongylidae) based on 66-kDa protein gene

Cited by 9 publications

References 32 publications

Phylogeography and population differentiation in Hepatozoon canis (Apicomplexa: Hepatozoidae) reveal expansion and gene flow in world populations

Phylogeography and population differentiation in Hepatozoon canis (Apicomplexa: Hepatozoidae) reveal expansion and gene flow in world populations

Genetic characterization of Angiostrongylus larvae and their intermediate host, Achatina fulica, in Thailand

Identification and genetic characterization of Angiostrongylus cantonensis isolated from the human eye

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