Transcriptional response of Mycobacterium sp. strain A1-PYR to multiple polycyclic aromatic hydrocarbon contaminations

Yuan, Ke; Xie, Xiuqin; Wang, Xiaowei; Lin, Li; Yang, Lihua; Luan, Tiangang; Chen, Baowei

doi:10.1016/j.envpol.2018.09.001

Cited by 24 publications

(9 citation statements)

References 64 publications

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“…The increase of extracellular protein in the first 3 days in this study (Figure 4) was positively correlated with the degradation efficiency in Figure 4a, which demonstrated that the bacterial cells had a stable cell activity and degradation capacity under BaP stress (Bacosa et al, 2018). The increase in extracellular proteins implied that S3), which demonstrated that these enzymes might involve in the process of BaP degradation (Kim et al, 2006;Yuan et al, 2018).…”

Section: Discussionsupporting

confidence: 56%

The isolation of benzo[a]pyrene‐degrading strain and its cometabolic bioremediation with salicylic acid of long‐term PAH‐polluted soil

Xie,

Zhang,

Liu

et al. 2023

Land Degrad Dev

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Polycyclic aromatic hydrocarbons (PAHs) are typical contaminants in soil, and the high‐molecular‐weight (HMW) PAHs are especially recalcitrant to remove. Microbial degradation as a promising approach has been used in PAH‐polluted soil remediation; however, the removal of HMW PAHs still needs to be further explored. In order to isolate more HMW‐PAH‐degrading strains and improve the removal of HMW PAHs in long‐term contaminated soil. Benzo[a]pyrene (BaP), as a representative HMW PAH was selected to explore the removal of HMW PAHs from soil. A BaP‐degrading strain named Achromobacter xylosoxidans B‐2 was isolated, and its degradation ability was explored in mineral salt medium (MSM) and PAH‐contaminated soil. Cosubstrates were added to enhance the removal of PAHs in soil. The results showed that A. xylosoxidans B‐2 could remove 87% of BaP at a concentration of 1 mg/L within 15 days in MSM. The average removal efficiency of HMW PAHs in soil was 19.92% after 30‐day bioremediation by A. xylosoxidans B‐2. Salicylic acid as cosubstrate increased the removal efficiency by 11.32%–14.66% for five‐ring PAHs and 9.72%–13.02% for six‐ring PAHs, respectively. The addition of cosubstrate not only improved the soil properties but also stimulated the soil microbial activity to enhance the removal of HMW PAHs. This study may provide new insights into bioremediation for HMW‐PAH‐contaminated soil.

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Section: Discussionsupporting

confidence: 56%

The isolation of benzo[a]pyrene‐degrading strain and its cometabolic bioremediation with salicylic acid of long‐term PAH‐polluted soil

Xie,

Zhang,

Liu

et al. 2023

Land Degrad Dev

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“…Genome-wide expression pro ling by DNA microarray analyses or next-generation sequencing techniques has been used to study many aromatic compound-degrading bacteria, including Bacillus subtilis NCIB 3610 (hydroxylated PCBs, methoxylated PCBs, and PCBs) [4], Bradyrhizobium japonicum USDA110 (4-hydroxybenzoate: 4-HBA, protocatechuate, vanillate, and vanillin) [5], Comamonas testosteroni WDL7 (3-chloroaniline) [6], Cupriavidus pinatubonensis JMP134 (2,4-dichlorophenoxyacetic acid: 2,4-D) [7], Mycobacterium sp. A1-PYR (phenanthrene and pyrene) [8], Novosphingobium sp. LH128 (phenanthrene) [9], Paraburkholderia xenovorans LB400 (benzoate: BA, biphenyl, PCBs, and phenylacetate) [10][11][12][13], Pseudomonas putida (3-chlorobenzoate: 3-CB and carbazole) [14][15][16], Rhodococcus aetherivorans I24 (biphenyl and PCBs) [17], Rhodococcus jostii RHA1 (BA, biphenyl, ethylbenzene, phthalate, and terephthalate) [18][19][20], Sinorhizobium meliloti 1021 (indole-3-acetic acid) [21], and Sphingobium chlorophenolicum L-1 (carbonyl cyanide m-chlorophenyl hydrazone, paraquat, pentachlorophenol, and toluene) [22].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome differences between Cupriavidus necator NH9 grown with 3-chlorobenzoate and that grown with benzoate

Moriuchi

Dohra

Kanesaki

et al. 2021

Bioscience, Biotechnology, and Biochemistry

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RNA-seq analysis of Cupriavidus necator NH9, a 3-chlorobenzoate degradative bacterium, cultured with 3-chlorobenzaote and benzoate, revealed strong induction of genes encoding enzymes in degradation pathways of the respective compound, including the genes to convert 3-chlorobenzaote and benzoate to chlorocatechol and catechol, respectively, and the genes of chlorocatechol ortho-cleavage pathway for conversion to central metabolites. The genes encoding transporters, components of the stress response, flagellar proteins, and chemotaxis proteins showed altered expression patterns between 3-chlorobenzoate and benzoate. Gene Ontology enrichment analysis revealed that chemotaxis related terms were significantly upregulated by benzoate compared with 3-chlorobenzoate. Consistent with this, in semi-solid agar plate assays, NH9 cells showed stronger chemotaxis to benzoate than to 3-chlorobenzoate. These results, combined with the absence of genes related to uptake/chemotaxis for 3-chlorobenzoate located closely to the degradation genes of 3-chlorobenzoate, suggested that NH9 has not fully adapted to the utilization of chlorinated benzoate, unlike benzoate, in nature.

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“…This study used multiple approaches to perform the analysis of five isolates of M. austroafricanum acquired from a collection of environmental mycobacteria obtained from aquatic sources from a zoo in São Paulo, Brazil. The main focus was the evaluation of their capacity of PAH degradation, as previously observed for other isolates from this mycobacterial species [ 12 , 18 , 19 , 20 , 38 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

“…Then, the cultures were centrifuged at 4900× g for 10 min, and the precipitates were washed twice with sterile Milli-Q water. After this step, bacterial suspensions with an optical density of 0.2 (OD 660 nm ) were prepared in Bushnell–Haas (BH) medium containing 10 mg/L of pyrene with or without 0.5% peptone [ 37 , 38 ]. As abiotic controls for this assay, incubations were performed with: (1) BH medium and pyrene and (2) BH medium, pyrene, and 0.5% peptone.…”

Section: Methodsmentioning

confidence: 99%

Multi-Approach Characterization of Novel Pyrene-Degrading Mycolicibacterium austroafricanum Isolates Lacking nid Genes

Silva,

Romagnoli,

Santiago

et al. 2023

Microorganisms

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Polycyclic aromatic hydrocarbons (PAHs) are chemical compounds that are widespread in the environment, arising from the incomplete combustion of organic material, as well as from human activities involving petrol exploitation, petrochemical industrial waste, gas stations, and environmental disasters. PAHs of high molecular weight, such as pyrene, have carcinogenic and mutagenic effects and are considered pollutants. The microbial degradation of PAHs occurs through the action of multiple dioxygenase genes (nid), which are localized in genomic island denominate region A, and cytochrome P450 monooxygenases genes (cyp) dispersed in the bacterial genome. This study evaluated pyrene degradation by five isolates of Mycolicibacterium austroafricanum using 2,6-dichlorophenol indophenol (DCPIP assay), gas chromatography/mass spectrometry (CG/MS), and genomic analyses. Two isolates (MYC038 and MYC040) exhibited pyrene degradation indexes of 96% and 88%, respectively, over a seven-day incubation period. Interestingly, the genomic analyses showed that the isolates do not have nid genes, which are involved in PAH biodegradation, despite their ability to degrade pyrene, suggesting that degradation may occur due to the presence of cyp150 genes, or even genes that have not yet been described. To the best of our knowledge, this is the first report of isolates without nid genes demonstrating the ability to degrade pyrene.

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Transcriptional response of Mycobacterium sp. strain A1-PYR to multiple polycyclic aromatic hydrocarbon contaminations

Cited by 24 publications

References 64 publications

The isolation of benzo[a]pyrene‐degrading strain and its cometabolic bioremediation with salicylic acid of long‐term PAH‐polluted soil

The isolation of benzo[a]pyrene‐degrading strain and its cometabolic bioremediation with salicylic acid of long‐term PAH‐polluted soil

Transcriptome differences between Cupriavidus necator NH9 grown with 3-chlorobenzoate and that grown with benzoate

Multi-Approach Characterization of Novel Pyrene-Degrading Mycolicibacterium austroafricanum Isolates Lacking nid Genes

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