A full-automated magnetic particle-based chemiluminescence immunoassay for rapid detection of cortisol in milk

Fu, Hui-Jun; Liu, Yuan; Shen, Yu-Dong; Liu, Yixin; Liu, Bo; Zhang, Shiwei; Xie, Zhuo-Xun; Lei, Hongtao; Sun, Yuan; Xu, Zhen-Lin

doi:10.1016/j.aca.2018.06.015

Cited by 22 publications

(9 citation statements)

References 31 publications

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“…Es por ello que la respuesta al estrés puede evaluarse analizando la cantidad de cortisol presente en el organismo, a través de distintas fracciones orgánicas tales como la sangre, la saliva o la leche. Actualmente, existen distintas tecnologías con potencial para medir dicha hormona de forma continua y automática (Fu et al, 2018). Es probable que el desarrollo de estas tecnologías permita, en un futuro próximo, calcular las concentraciones de cortisol en muestras de leche de forma sistematizada, lo que permitirá detectar niveles de estrés incrementados en vacas lecheras en tiempo real.…”

Section: Aumento De La Temperatura Atmosféricaunclassified

Impacto del cambio climático sobre el bienestar animal en los sistemas ganaderos

Blanco‐Penedo¹,

Cantalapiedra²,

Llonch³

2020

ITEA

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El cambio climático global afecta cada vez más a la producción agrícola y ganadera con un efecto negativo abrumador en la salud y el bienestar del ganado. El sector ganadero tiene la urgencia de ser más eficiente y sostenible y cumplir con estándares más altos de bienestar animal. Los efectos negativos del cambio climático sobre la salud y el bienestar de los animales son la consecuencia de los cambios combinados de la temperatura atmosférica, precipitaciones, y la frecuencia y magnitud de los fenómenos meteorológicos extremos que pueden ser tanto directos como indirectos. Las prácticas agroganaderas deben considerar la necesidad de adaptación continua (resiliencia) a un entorno en constante cambio que ofrezca soluciones para amortiguar los eventos extremos climáticos, la disponibilidad cambiante de nutrientes, la disponibilidad estacional de forraje, la epidemiología de las enfermedades, y otras tensiones que se sumarán a un entorno de condiciones heterogéneas. Esta revisión pretende documentar el conocimiento científico más actualizado sobre el impacto del cambio climático en la salud y el bienestar animal, las estrategias de adaptación de los sistemas ganaderos que pueden implementarse para reducir este impacto, así como identificar las oportunidades para investigar nuevas estrategias de adaptación.

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Section: Aumento De La Temperatura Atmosféricaunclassified

Impacto del cambio climático sobre el bienestar animal en los sistemas ganaderos

Blanco‐Penedo¹,

Cantalapiedra²,

Llonch³

2020

ITEA

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“…Conventional immunoassays based on colorimetric or luminescence readout are most often based on laboratory operations as well as professional instruments, , which usually provide them with a high diagnostic performance but also with shortcomings concerning on-site sample analysis. Naked-eye or smartphone-based analyses are usually less precise but may be sufficient for certain applications and come with the great advantage of simplicity, low cost, and rapidity for on-site monitoring.…”

Section: Introductionmentioning

confidence: 99%

Enzyme-Induced Silver Deposition on Gold Nanorods for Naked-Eye and Smartphone Detection of Acrylamide in Food

Luo

Wang

et al. 2022

ACS Appl. Nano Mater.

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Acrylamide is a probable human carcinogen with a wide occurrence in commonly consumed food. Because of the lack of specific antibodies against acrylamide, immunoassays usually measure acrylamide derivates, which require long derivatization times at elevated temperatures. One exception is xanthylacrylamide (XAA), which can be derived from acrylamide at room temperature within 30 min. Here, we present the development of a monoclonal antibody against XAA and a competitive enzyme-linked immunosorbent assay (ELISA) based on the multicolor detection of gold nanorods (AuNRs). The biotinylated monoclonal antibodies bound streptavidin alkaline phosphatase, which induced XAA concentration-dependent silver deposition on the AuNRs. The resulting blue shift of the AuNR surface plasmon resonance peak translated into a strong gray-to-orange color change that could be conveniently distinguished by the naked eye or smartphone color detection. The assay showed a broad linear range (0.3–17.2 ng/mL), a low limit of detection (0.06 ng/mL), and a 3.6-fold sensitivity improvement compared to those of conventional tetramethylbenzidine-based ELISA. The analysis of several different food products showed excellent acrylamide concentration recoveries (84–102%) and confirmed its applicability as an effective tool for routine monitoring of acrylamide in food. Rapid and simple color analysis demonstrated a strong potential for implementation into direct consumer product testing.

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“…The immunosensor also has a short shelf-life; long-term storage may deteriorate the performance of detection . Apart from antibody-based assays, cortisol-selective aptamers have been demonstrated for cortisol detection accompanied by additional labeling for electrochemical or optical sensing readouts. ,, Nucleic acid aptamers are relatively stable at room temperature and are accessible for various ionic buffers, making them suitable for the applications in point-of-care diagnostics. − …”

Section: Introductionmentioning

confidence: 99%

“…11 Apart from antibody-based assays, cortisol-selective aptamers have been demonstrated for cortisol detection accompanied by additional labeling for electrochemical or optical sensing readouts. 2,12,13 Nucleic acid aptamers are relatively stable at room temperature and are accessible for various ionic buffers, making them suitable for the applications in point-of-care diagnostics. 14−20 A variety of biosensors have been reported for sensitive salivary cortisol detection, such as surface plasmon resonancebased sensors, 21,22 electrochemical sensors, 23,24 and colorimetric sensors based on molecularly imprinted polymer.…”

Section: ■ Introductionmentioning

confidence: 99%

Label-Free Sensitive Detection of Steroid Hormone Cortisol Based on Target-Induced Fluorescence Quenching of Quantum Dots

Liu

Tanyi

et al. 2020

Langmuir

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We discovered that several types of steroid hormones quench the fluorescence of quantum dots (QDs) at close proximity. Inspired by the finding, we developed a new type of biosensor for the sensitive detection of cortisol via direct fluorescence quenching of functionalized QD probes directly induced by the capture of target cortisol without additional reporter reagents. The detection selectivity was provided by cortisol-selective aptamers or anticortisol antibodies conjugated on the QD surfaces. With the magnetic nanoparticle labeling, the new sensing method enabled rapid cortisol sensing at physiologically relevant concentrations and yielded the detection limit of ∼1 nM for aptamer-based sensors and ∼100 pM for antibody-based sensors. We also evaluated the new detection method using saliva samples with an optimized sample preparation process under the assistance of magnetic manipulation. The result showed a satisfying recovery rate for spiked saliva tests. The facile sensing technology offers an appealing approach for the detection of steroid hormones in point-of-care settings.

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A full-automated magnetic particle-based chemiluminescence immunoassay for rapid detection of cortisol in milk

Cited by 22 publications

References 31 publications

Impacto del cambio climático sobre el bienestar animal en los sistemas ganaderos

Impacto del cambio climático sobre el bienestar animal en los sistemas ganaderos

Enzyme-Induced Silver Deposition on Gold Nanorods for Naked-Eye and Smartphone Detection of Acrylamide in Food

Label-Free Sensitive Detection of Steroid Hormone Cortisol Based on Target-Induced Fluorescence Quenching of Quantum Dots

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