MMP-11 as a biomarker for metastatic breast cancer by immunohistochemical-assisted imaging mass spectrometry

Vega, Raquel González de; Clases, David; Sánchez, Marı́a Luisa Fernández; Eiró, Noemí; González, Luis O.; Vizoso, F; Doble, Philip; Sanz‐Medel, Alfredo

doi:10.1007/s00216-018-1365-3

Cited by 41 publications

(27 citation statements)

References 21 publications

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“… 102 Research of González de Vega et al confirmed that the expression of MMP-11 was elevated in metastatic BC than non-metastatic tumor samples. 103 …”

Section: Mmp-11 In Carcinogenesis Of Cancersmentioning

confidence: 99%

<p>Matrilysins and Stromelysins in Pathogenesis and Diagnostics of Cancers</p>

Piskór¹,

Przylipiak²,

Dąbrowska³

et al. 2020

CMAR

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Matrix metalloproteinases (MMPs) are endopeptidases which are widely studied in terms of their role in the physiological and pathological processes in the organism. In this article, we consider usefulness of matrilysins and stromelysins in pathogenesis and diagnostic of the most common malignancies in the world, e.g., lung, breast, prostate, and colorectal cancers. In all of the mentioned cancers, matrilysins and stromelysins have a pivotal role in their development and also may have diagnostic utility. Influence to the cancerous process is connected with specific dependencies between these enzymes and components of the extracellular matrix (ECM), non-matrix components like cell surface components. All the information provided below allows to take a closer look at matrilysins and stromelysins and their functions in the cancer development.

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“… 102 Research of González de Vega et al confirmed that the expression of MMP-11 was elevated in metastatic BC than non-metastatic tumor samples. 103 …”

Section: Mmp-11 In Carcinogenesis Of Cancersmentioning

confidence: 99%

<p>Matrilysins and Stromelysins in Pathogenesis and Diagnostics of Cancers</p>

Piskór¹,

Przylipiak²,

Dąbrowska³

et al. 2020

CMAR

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“…MMP11 serves as an endopeptidase‐degrading extracellular matrix (Gómez‐Macías et al , 2018). MMP11 overexpression in CAFs and cancer cells has previously been described to correlate with an aggressive cancer profile and promotion of metastasis (González de Vega et al , 2019; Peruzzi et al , 2009). Further, an upregulation of glycoprotein CD38 was detected in the sorted immune cells from EAC tumors ( P = 8.6 × 10 −7 , Fisher's exact test).…”

Section: Resultsmentioning

confidence: 94%

Cell type‐specific transcriptomics of esophageal adenocarcinoma as a scalable alternative for single cell transcriptomics

et al. 2020

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As a proof of concept, we profiled tumor samples of nine patients and explored expression differences in the three cell populations between tumor and normal tissue. Interestingly, we found that angiogenesis-related genes were upregulated in fibroblasts isolated from tumors compared with normal tissue. Overall, we suggest our protocol as a complementary and more scalable approach compared with single-cell RNA sequencing to investigate associations between clinical parameters and transcriptomic alterations of specific cell populations in esophageal adenocarcinoma.

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“…Historically, antibodies are the most commonly used group of biorecognition elements. Therefore, antibodies labelled by an elemental tag-chelate binding rare earth elements [3,4] enabled to detect specific proteins (e.g., p53 [5], ferroportin [6], MMP-11 [7], etc.) in levels of pg [8] by LA-ICP-MS. Also, currently available commercial CyTOF instruments rely on the use of antibodies [9,10].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection

et al. 2021

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Immunochemical methods are used not only in clinical practice for the diagnosis of a wide range of diseases but also in basic and advanced research. Based on the unique reaction between the antibody and its respective antigens, it serves to specifically recognize target molecules in biological complex samples. Current methods of labelling antibodies with elemental labels followed by detection by inductively coupled plasma mass spectrometry (ICP-MS) allow detection of multiple antigens in parallel in a single analysis. Using the laser ablation (LA) modality (LA-ICP-MS), it is also possible to monitor the spatial distribution of biogenic elements. Moreover, the employment of metal nanoparticle-labeled antibodies expands the applicability also to molecular imaging by LA-ICP-MS. In this work, conjugates of model monoclonal antibody (DO-1, recognizing p53 protein) with various metal nanoparticles-based labels were created and utilized in dot-blot analysis in order to compare their benefits and disadvantages. Based on experiments with the p53 protein standard, commercial kits of gold nanoparticles proved to be the most suitable for the preparation of conjugates. The LA-ICP-MS demonstrated very good repeatability, wide linear dynamic range (0.1–14 ng), and limit of detection was calculated as a 1.3 pg of p53 protein.

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MMP-11 as a biomarker for metastatic breast cancer by immunohistochemical-assisted imaging mass spectrometry

Cited by 41 publications

References 21 publications

<p>Matrilysins and Stromelysins in Pathogenesis and Diagnostics of Cancers</p>

<p>Matrilysins and Stromelysins in Pathogenesis and Diagnostics of Cancers</p>

Cell type‐specific transcriptomics of esophageal adenocarcinoma as a scalable alternative for single cell transcriptomics

Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection

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