Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)

Castro, Talita; Sabalza, Maite; Barber, Cheryl; Abrams, William R.; Costa, Antônio Charlys da; Milagres, Flavio Augusto de Pádua; Braz‐Silva, Paulo Henrique; Malamud, Daniel; Gallottini, Marina

doi:10.1080/20002297.2018.1510712

Cited by 25 publications

(30 citation statements)

References 24 publications

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“…Using 131 clinical samples from suspected patients collected during the acute phase of the disease, including 68 saliva and 63 urine specimens, an RT-LAMP assay was able to detect the ZIKV in 53.4% of the total number of samples. Interestingly, this study showed that ZIKV detection using saliva had a faster time to positivity compared to urine samples [61].…”

Section: Lamp Assay Evaluation Using Clinical Samplesmentioning

confidence: 67%

“…To provide perspective on the analytical sensitivity of different LAMP assays for ZIKV, we assembled a table summarizing this measure for all studies using ZIKV-spiked or RNA-spiked samples (Table 1) [27,28,[37][38][39][40][41][42]44,53,[57][58][59][60][61][62][63][64]. The top-performing ZIKV RT-LAMP method could detect ZIKV RNA standard at as few as 0.02 PFU/test, using RNA prepared by in vitro transcription.…”

Section: Lamp Sensitivitymentioning

confidence: 99%

“…The suitability of LAMP assays for ZIKV detection in clinical samples has been evaluated in several studies using approximately 800 selected samples from patients with suspected arboviruses infection in Brazil, Nicaragua, and the USA [28,41,42,59,61]. For comparison, clinical samples were also assayed using RT-qPCR.…”

Section: Lamp Assay Evaluation Using Clinical Samplesmentioning

confidence: 99%

“…Other previously published papers also evaluated the efficacy of the RT-LAMP for diagnosis of the ZIKV in clinical samples and compared these results with RT-qPCR [41,42,61]. Using 131 clinical samples from suspected patients collected during the acute phase of the disease, including 68 saliva and 63 urine specimens, an RT-LAMP assay was able to detect the ZIKV in 53.4% of the total number of samples.…”

Section: Lamp Assay Evaluation Using Clinical Samplesmentioning

confidence: 99%

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Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Silva

Pardee

Pena

2019

Viruses

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The recent outbreak of Zika virus (ZIKV) in the Americas and its devastating developmental and neurological manifestations has prompted the development of field-based diagnostics that are rapid, reliable, handheld, specific, sensitive, and inexpensive. The gold standard molecular method for lab-based diagnosis of ZIKV, from either patient samples or insect vectors, is reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The method, however, is costly and requires lab-based equipment and expertise, which severely limits its use as a point-of-care (POC) tool in resource-poor settings. Moreover, given the lack of antivirals or approved vaccines for ZIKV infection, a POC diagnostic test is urgently needed for the early detection of new outbreaks and to adequately manage patients. Loop-mediated isothermal amplification (LAMP) is a compelling alternative to RT-qPCR for ZIKV and other arboviruses. This low-cost molecular system can be freeze-dried for distribution and exhibits high specificity, sensitivity, and efficiency. A growing body of evidence suggests that LAMP assays can provide greater accessibility to much-needed diagnostics for ZIKV infections, especially in developing countries where the ZIKV is now endemic. This review summarizes the different LAMP methods that have been developed for the virus and summarizes their features, advantages, and limitations.Viruses 2020, 12, 19 2 of 20 raising concerns about the neurological tropism of the virus [12]. In May 2015, the first case of ZIKV infection was reported in Brazil [13] and the virus rapidly spread throughout the country and much of Latin America, causing the largest recorded epidemic of the virus to date [14]. The Brazilian epidemic raised great international concern because of severe birth defects, including microcephaly, in neonates born to mothers infected by ZIKV during pregnancy [3,15,16].ZIKV is transmitted mainly through the bite of infected mosquitoes from the genus Aedes, although other vectors may also be involved in the transmission [17][18][19][20][21]. Additionally, other routes of ZIKV transmission have been identified, including blood transfusions, transplacental, perinatal, and sexual intercourse [22][23][24]. ZIKV infection usually causes a self-limiting and a mild illness, where the majority of cases are asymptomatic and, when present, symptoms include fever, headache, rash, conjunctivitis, and arthralgia [25]. In regions where there is a circulation of other arboviruses, such as DENV and chikungunya (CHIKV), the clinical diagnosis of ZIKV infection becomes extremely difficult because of common symptoms. Therefore, laboratory-based molecular diagnosis is of fundamental importance to correctly identify the etiologic agent [3,26].Given the lack of approved vaccines and antivirals against ZIKV, a rapid and reliable point-of-care (POC) diagnostic test for detection of ZIKV is urgently required for control and prevention measures and to increase the diagnostic capacity of ZIKV-affected, mainly in low-resource areas [27,28]. Z...

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Section: Lamp Assay Evaluation Using Clinical Samplesmentioning

confidence: 67%

Section: Lamp Sensitivitymentioning

confidence: 99%

Section: Lamp Assay Evaluation Using Clinical Samplesmentioning

confidence: 99%

Section: Lamp Assay Evaluation Using Clinical Samplesmentioning

confidence: 99%

See 2 more Smart Citations

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Silva

Pardee

Pena

2019

Viruses

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“…[7,10]. Thus, LAMP could be a highly beneficial and convenient application to detect Zika virus due to its high specificity and stability, and it has been reported that LAMP shined a light on Zika virus [11][12][13][14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Sensitive and rapid detection of Zika virus by loop-mediated isothermal amplification

Zhao

Feng

2018

Virus Genes

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Zika virus (ZIKV) is a mosquito-borne flavivirus, which is a pathogen affecting humans in Africa, Asia, and America. It is necessary to detect ZIKV with a rapid and sensitive molecular method to guide timely treatment. In this study, a loopmediated isothermal amplification (LAMP) assay was described, which is an attractive option as a fast, sensitive, and specific method for ZIKV detection using the NS5 protein coding region and the envelope protein (EP) coding region as target sequences. Two different techniques, a calcein/Mn 2+ complex chromogenic method and real-time turbidity monitoring, were employed. The specificity and sensitivity of the LAMP assay were determined. The assay's detection limit was 0.5 × 10 −9 pmol/µl DNA for NS5 protein coding region and 1.12 × 10 −11 pmol/µl DNA for E coding region, respectively, which is a 100-fold increase in sensitivity compared with real-time reverse transcription-polymerase chain reaction (RT-PCR) and conventional PCR. All 12 non-ZIKA respiratory pathogens tested were negative for LAMP detection, indicating the high specificity of the primers for ZIKV. In conclusion, a visual detection LAMP assay was developed, which could be a useful tool for primary quarantine purposes and clinical screening, especially in situations where resources are poor and in point-of-care tests.

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Biomarker based biosensors: An opportunity for diagnosis of COVID‐19

Khaksarinejad

Arabpour

Rezakhani

et al. 2022

Reviews in Medical Virology

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Early diagnosis and treatment of diseases are crucial research areas of human health. For early diagnosis, one method that has proven efficient is the detection of biomarkers which can provide real‐time and accurate biological information. Most biomarker detection is currently carried out at localised dedicated laboratories using large and automated analysers, increasing waiting time and costs. Smaller, faster, and cheaper devices could potentially replace these time‐consuming laboratory analyses and make analytical results available as point‐of‐care diagnostics. Innovative biosensor‐based strategies could allow biomarkers to be tested reliably in a decentralised setting. Early diagnosis of COVID‐19 patients has a key role in order to use quarantine and treatment strategies in a timely manner. Raised levels of several biomarkers in COVID‐19 patients are associated with respiratory infections or dysfunction of various organs. Through clinical studies of COVID‐19 patient biomarkers such as ferritin, Interleukins, albumin and …are found to reveals significant differences in their excretion ranges from healthy patients and patients with SARS‐CoV‐2, in addition to the development of biomarkers based biosensor such as stated biomarkers can be used and to investigate more specific biomarkers further proteomic analysis can be performed. This review presents several biomarker alterations in COVID‐19 patients such as salivary, circulatory, coagulation, cardiovascular, renal, liver, C‐reactive protein (CRP), immunological and inflammatory biomarkers. Also, biomarker sensors based on electrochemical, optical, and lateral flow characteristics which have potential applications for SARS‐COV‐2 in the recent COVID‐19 pandemic, will be discussed.

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Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)

Cited by 25 publications

References 24 publications

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Sensitive and rapid detection of Zika virus by loop-mediated isothermal amplification

Biomarker based biosensors: An opportunity for diagnosis of COVID‐19

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