Equine immunoglobulin F(ab′)2 fragments protect mice from Rift Valley fever virus infection

Zhao, Yongkun; Zheng, Xiaodong; He, Shihua; Li, Yuetao; Wang, Wei; Gai, Weiwei; Wong, Gary; Wang, Hualei; Yan, Feihu; Xue, Feng; Feng, Na; Wang, Tiecheng; Gao, Yuwei; Wang, Cuiling; Qiu, Xiangguo; Xia, Xianzhu

doi:10.1016/j.intimp.2018.09.002

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…Cats in the other groups that died were those with higher levels of the virus in their group. The higher the virus content, the earlier the death Equine immunoglobulin F(ab′) 2 fragments, being bivalent, are easy to purify in large quantities, more able to penetrate deeper into the tissue than the IgG antibody [6] and have been reported to be able to prevent avian influenza A virus (H5N1) [27], feline calicivirus [8] and Rift Valley fever virus [28]. In dealing with feline calicivirus, 0 mg/kg, 1 mg/kg, 2 mg/kg and 5 mg/kg of anti-FCV pF(ab′) 2 were administered to cats the day before FCV challenge, and the survival rate was 20%, 80%, 100% and 100% at the 14 days after FCV challenge, respectively [8].…”

Section: Discussionmentioning

confidence: 99%

Prophylactic Efficacy of Equine Immunoglobulin F(ab′)2 Fragments Against Feline Parvovirus

Liu

Zhang

Bai

et al. 2021

Appl Biochem Biotechnol

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Feline parvovirus (FPV), a type of parvovirus prevalent worldwide, can cause foetal death and acute enteritis in adult cats with severe leukopenia, and yet there are no effective drugs to prevent or treat FPV. Here, the immune effects of two FPV vaccines on horses were compared. IgG was extracted from FPV-immunized horse sera. Equine F(ab′) 2 fragments were obtained from pepsin-digested IgG and then purified by protein-G column chromatography. The results showed that the inactivated FPV oil vaccine was more effective than the inactivated FPV propolis vaccine in helping healthy horses to produce hyper-immune serum. Four methods were tested, among which the optimized octanoic acid-ammonium sulphate precipitation method was proved to be the best process for extracting IgG. The optimal condition for preparing F(ab′) 2 by pepsin digestion was 30°C for 3.5 h, and the content, purity and recovery of F(ab′) 2 were 8.64 mg/mL, 90.36% and 93.24%, respectively. Our equine immunoglobulin F(ab′) 2 fragments effectively neutralized activity in vitro against FPV, alleviated the clinical symptoms of FPVinfected cats, reduced the viral loads in the intestine and had prophylactic effects in FPV-infected cats. These results indicate that the F(ab′) 2 fragment prepared from inactivated FPV-immunized horses may be used as a prophylactic agent for diseases caused by FPV.

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Section: Discussionmentioning

confidence: 99%

Prophylactic Efficacy of Equine Immunoglobulin F(ab′)2 Fragments Against Feline Parvovirus

Liu

Zhang

Bai

et al. 2021

Appl Biochem Biotechnol

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“…Female, 6-8 weeks-old BALB/c mice with a body weight of 15-16 g were purchased from Changchun Yisi Experimental Animal Co., Ltd., China. The mice were maintained as our previous study (10). All animal feeding was in compliance with animal welfare regulations.…”

Section: Experimental Animalsmentioning

confidence: 99%

Immunogenicity Assessment of Rift Valley Fever Virus Virus-Like Particles in BALB/c Mice

Han

Zhao

et al. 2020

Front. Vet. Sci.

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Rift Valley fever (RVF) is an acute, febrile zoonotic disease that is caused by the RVF virus (RVFV) and is spread by arthropod vectors. Virus-like particle (VLP) vaccines, which have the advantages of strong immunogenicity and safety, play an important role in the prevention of this disease. VLPs for RVFV were successfully prepared by our research group using a baculovirus-insect cell expression system. To study the immunogenicity of these RVFV VLPs, a correct 3rd or 4th generation recombinant baculovirus, rBac-N-G, was identified and used to infect Sf9 cells, which were cultured in suspension at a large scale. Subsequently, cell debris was removed by centrifugation, and the VLPs were concentrated by ultracentrifugation and purified using a sucrose gradient, after which they were used to immunize BALB/c mice by intramuscular injection. The results showed that the RVFV VLPs prepared by our research group could effectively induce mice to produce RVFV neutralizing antibodies and that the prepared VLPs could stimulate mouse spleen cells to produce high levels of the cytokines IL-4 and IFN-γ. Moreover, the proportion of lymphocytes producing IL-4 and IFN-γ in the spleen of mice immunized with RVFV VLPs was significantly increased. Therefore, the RVFV VLPs prepared in this study had strong immunogenicity and could effectively activate humoral and cellular immunity in mice. This study lays a solid foundation for the development of RVFV VLP vaccine candidates and promotes the healthy development of animal husbandry and human public health.

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“…Animal-derived immunoglobulin such as equine sourced anti-SARS-CoV F(ab´)2 was reported to be effective in cell culture and murine and the hamster in vivo in vivo models [10 -11]. Moreover equine sourced F(ab´)2 was reported to protect mice from Rift Valley fever virus infection [12]. Dixit et al [13] concluded that equine polyclonal antibody therapies could also be adapted for widespread and severe neglected tropical diseases, for example, the viral hemorrhagic fevers like Crimean-Congo Haemorrhagic Fever, Dengue, Ebola, and Marburg; Bat-transmitted viruses such as Nipah and Hendra, as well as Lassa virus, West Nile Virus and SARS.…”

Section: Introductionmentioning

confidence: 99%

History repeats itself: horse originated hyperimmune sera production against SARS CoV-2

Pakdemirli

Caliskan²,

Hacıoğlu³

et al. 2021

Turk J Med Sci

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Background/aim: SARS-CoV-2 disease was announced as a pandemic by The World Health Organization in on early 2020. It is still threatening the world population. Here, we aimed to produce hyperimmune sera that contain immunoglobulin G and F(ab')2 fragments sourced from horse antibodies as an urgent response to the pandemic.Materials and methods: SARS-CoV-2 was produced and inactivated with three different methods [formaldehyde (FA), formaldehyde, and binary ethylene amine (FA+BEI), and heat treatment]. After invitro in vitro inactivation control, immunogens were mixed with Freund's adjuvant, thereafter horses (n: 2 for FA, 4 for FA + BEI, 2 for Heat inactivation) and New Zealand rabbits (n: 6 for FA, 6 for FA + BEI, 6 for Heat inactivation) were immunized four times. Neutralizing antibody levels of the sera were measured at the 4 th , 6 th , and 8 th weeks. When the antibodies were detected at the peak level, plasma was collected from horses and hyperimmune sera procured after the purification process.Results: Horses and rabbits produced highly neutralizing antibodies against the SARS-CoV-2 in FA and FA + BEI inactivation groups, foreign proteins were removed effectively after purification. Conclusion:This study presents a profitable practice to develop horse-specific antisera against SARS-CoV-2 for emergency and low-cost response. In further studies, new purification methods can be used to increase the efficiency of the final product.

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Equine immunoglobulin F(ab′)2 fragments protect mice from Rift Valley fever virus infection

Cited by 4 publications

References 31 publications

Prophylactic Efficacy of Equine Immunoglobulin F(ab′)2 Fragments Against Feline Parvovirus

Prophylactic Efficacy of Equine Immunoglobulin F(ab′)2 Fragments Against Feline Parvovirus

Immunogenicity Assessment of Rift Valley Fever Virus Virus-Like Particles in BALB/c Mice

History repeats itself: horse originated hyperimmune sera production against SARS CoV-2

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